Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly und...
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ftzenodo:oai:zenodo.org:4996572 2023-06-06T11:52:55+02:00 Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. 2018-10-25 https://zenodo.org/record/4996572 https://doi.org/10.5061/dryad.kn45n49 unknown doi:10.1098/rspb.2018.1676 https://zenodo.org/communities/dryad https://zenodo.org/record/4996572 https://doi.org/10.5061/dryad.kn45n49 oai:zenodo.org:4996572 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas info:eu-repo/semantics/other dataset 2018 ftzenodo https://doi.org/10.5061/dryad.kn45n4910.1098/rspb.2018.1676 2023-04-13T21:27:13Z Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. Electronic data for kinetics studyThe electronic raw data for kinetics experimentElectronic data caloxinElectronic data ORMElectronic data orm.xlsxElectronic data ouabainElectronic data vanadateElectronic data verapamilDPM kinetics studyDPM kinetics.xlsxDPM caloxinDPM ORMDPM ouabainDPM vanadateDPM verapamil Dataset Crassostrea gigas Pacific oyster Zenodo Pacific |
institution |
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collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas |
spellingShingle |
Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
topic_facet |
Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas |
description |
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. Electronic data for kinetics studyThe electronic raw data for kinetics experimentElectronic data caloxinElectronic data ORMElectronic data orm.xlsxElectronic data ouabainElectronic data vanadateElectronic data verapamilDPM kinetics studyDPM kinetics.xlsxDPM caloxinDPM ORMDPM ouabainDPM vanadateDPM verapamil |
format |
Dataset |
author |
Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. |
author_facet |
Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. |
author_sort |
Sillanpää, J. Kirsikka |
title |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_short |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_sort |
data from: calcium transfer across the outer mantle epithelium in the pacific oyster, crassostrea gigas |
publishDate |
2018 |
url |
https://zenodo.org/record/4996572 https://doi.org/10.5061/dryad.kn45n49 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.1098/rspb.2018.1676 https://zenodo.org/communities/dryad https://zenodo.org/record/4996572 https://doi.org/10.5061/dryad.kn45n49 oai:zenodo.org:4996572 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.kn45n4910.1098/rspb.2018.1676 |
_version_ |
1767958962753241088 |