Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms
Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 hours post fertilization, and it has been demonstrat...
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ftzenodo:oai:zenodo.org:4991055 2023-05-15T15:57:54+02:00 Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms De Wit, Pierre Durland, Evan Ventura, Alexander Langdon, Chris J. 2019-01-31 https://zenodo.org/record/4991055 https://doi.org/10.5061/dryad.8m5v4 unknown doi:10.1186/s12864-018-4519-y https://zenodo.org/communities/dryad https://zenodo.org/record/4991055 https://doi.org/10.5061/dryad.8m5v4 oai:zenodo.org:4991055 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode aragonite larvae calcification Crassostrea gigas info:eu-repo/semantics/other dataset 2019 ftzenodo https://doi.org/10.5061/dryad.8m5v410.1186/s12864-018-4519-y 2023-03-10T19:05:56Z Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 hours post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to pCO2-acidified seawater to identify genes correlated with initial shell deposition. By fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process. This work provides a foundation for further studies on how genetic variation in these identified genes could affect fitness of oyster populations subjected to future environmental changes, such as ocean acidification. Experiment 1 (NET3) Raw read counts (with duplicates)Experiment 1 (NET3) Raw read counts (with duplicates), mapped to the oyster genome v9 coding regions.NET3_Counts_with_dups.txtExperiment 2 (NET1) Raw read counts (with duplicates)Experiment 2 (NET1) Raw read counts (with duplicates), mapped against the oyster genome v9 coding regions.NET1_Counts_with_dups.txtExperiment 1 (NET3) Raw read counts (duplicates removed)Experiment 1 (NET3) Raw read counts (duplicates removed), mapped against the oyster genome v9 coding regionsNET3_Counts_dedup.txtExperiment 2 (NET1) Raw read counts (duplicates removed)Experiment 2 (NET1) Raw read counts (duplicates removed), mapped against the oyster ... Dataset Crassostrea gigas Ocean acidification Zenodo Pacific |
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unknown |
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aragonite larvae calcification Crassostrea gigas |
spellingShingle |
aragonite larvae calcification Crassostrea gigas De Wit, Pierre Durland, Evan Ventura, Alexander Langdon, Chris J. Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
topic_facet |
aragonite larvae calcification Crassostrea gigas |
description |
Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 hours post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to pCO2-acidified seawater to identify genes correlated with initial shell deposition. By fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process. This work provides a foundation for further studies on how genetic variation in these identified genes could affect fitness of oyster populations subjected to future environmental changes, such as ocean acidification. Experiment 1 (NET3) Raw read counts (with duplicates)Experiment 1 (NET3) Raw read counts (with duplicates), mapped to the oyster genome v9 coding regions.NET3_Counts_with_dups.txtExperiment 2 (NET1) Raw read counts (with duplicates)Experiment 2 (NET1) Raw read counts (with duplicates), mapped against the oyster genome v9 coding regions.NET1_Counts_with_dups.txtExperiment 1 (NET3) Raw read counts (duplicates removed)Experiment 1 (NET3) Raw read counts (duplicates removed), mapped against the oyster genome v9 coding regionsNET3_Counts_dedup.txtExperiment 2 (NET1) Raw read counts (duplicates removed)Experiment 2 (NET1) Raw read counts (duplicates removed), mapped against the oyster ... |
format |
Dataset |
author |
De Wit, Pierre Durland, Evan Ventura, Alexander Langdon, Chris J. |
author_facet |
De Wit, Pierre Durland, Evan Ventura, Alexander Langdon, Chris J. |
author_sort |
De Wit, Pierre |
title |
Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_short |
Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_full |
Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_fullStr |
Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_full_unstemmed |
Data from: Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_sort |
data from: gene expression correlated with delay in shell formation in larval pacific oysters (crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
publishDate |
2019 |
url |
https://zenodo.org/record/4991055 https://doi.org/10.5061/dryad.8m5v4 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification |
genre_facet |
Crassostrea gigas Ocean acidification |
op_relation |
doi:10.1186/s12864-018-4519-y https://zenodo.org/communities/dryad https://zenodo.org/record/4991055 https://doi.org/10.5061/dryad.8m5v4 oai:zenodo.org:4991055 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.8m5v410.1186/s12864-018-4519-y |
_version_ |
1766393613916307456 |