Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area...
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ftzenodo:oai:zenodo.org:4989228 2024-09-15T18:10:05+00:00 Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. 2015-11-18 https://doi.org/10.5061/dryad.sg5s0 unknown Zenodo https://doi.org/10.1111/1755-0998.12489 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.sg5s0 oai:zenodo.org:4989228 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode DNA barcode library info:eu-repo/semantics/other 2015 ftzenodo https://doi.org/10.5061/dryad.sg5s010.1111/1755-0998.12489 2024-07-26T02:02:22Z DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. DS-ZACKANIM intrasp divergence and distance to NN raw data Raw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKANIM (dx.doi.org/10.5883/DS-ZACKANIM) and the DNA barcode region CO1 DS-ZACKPLAN ITS2 intrasp divergence and distance to NN raw data Raw data on ... Other/Unknown Material Greenland Zackenberg Zenodo |
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unknown |
topic |
DNA barcode library |
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DNA barcode library Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
topic_facet |
DNA barcode library |
description |
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. DS-ZACKANIM intrasp divergence and distance to NN raw data Raw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKANIM (dx.doi.org/10.5883/DS-ZACKANIM) and the DNA barcode region CO1 DS-ZACKPLAN ITS2 intrasp divergence and distance to NN raw data Raw data on ... |
format |
Other/Unknown Material |
author |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. |
author_facet |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. |
author_sort |
Wirta, H. |
title |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_short |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_fullStr |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full_unstemmed |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_sort |
data from: establishing a community-wide dna barcode library as a new tool for arctic research |
publisher |
Zenodo |
publishDate |
2015 |
url |
https://doi.org/10.5061/dryad.sg5s0 |
genre |
Greenland Zackenberg |
genre_facet |
Greenland Zackenberg |
op_relation |
https://doi.org/10.1111/1755-0998.12489 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.sg5s0 oai:zenodo.org:4989228 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.sg5s010.1111/1755-0998.12489 |
_version_ |
1810447690370121728 |