Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus)
The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is requi...
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ftzenodo:oai:zenodo.org:4989011 2023-06-06T11:56:18+02:00 Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique 2018-04-11 https://zenodo.org/record/4989011 https://doi.org/10.5061/dryad.d4368 unknown doi:10.1002/ece3.2943 https://zenodo.org/communities/dryad https://zenodo.org/record/4989011 https://doi.org/10.5061/dryad.d4368 oai:zenodo.org:4989011 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode info:eu-repo/semantics/other dataset 2018 ftzenodo https://doi.org/10.5061/dryad.d436810.1002/ece3.2943 2023-04-13T21:27:50Z The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with non-invasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totalling 126 alleles and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares. Additional File_M1 Dataset Lepus timidus mountain hare Zenodo |
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unknown |
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The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with non-invasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totalling 126 alleles and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares. Additional File_M1 |
format |
Dataset |
author |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique |
spellingShingle |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
author_facet |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique |
author_sort |
Beugin, Marie-Pauline |
title |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_short |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_full |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_fullStr |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_full_unstemmed |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_sort |
data from: a single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (lepus europaeus) and the mountain hare (lepus timidus) |
publishDate |
2018 |
url |
https://zenodo.org/record/4989011 https://doi.org/10.5061/dryad.d4368 |
genre |
Lepus timidus mountain hare |
genre_facet |
Lepus timidus mountain hare |
op_relation |
doi:10.1002/ece3.2943 https://zenodo.org/communities/dryad https://zenodo.org/record/4989011 https://doi.org/10.5061/dryad.d4368 oai:zenodo.org:4989011 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.d436810.1002/ece3.2943 |
_version_ |
1767963724001312768 |