FT TFL1 bimolecular luciferase complementation assays
CORRECTION Ho, W.W.H., and Weigel, D. (2014). Structural Features Determining Flower-Promoting Activity of Arabidopsis FLOWERING LOCUS T. Plant Cell 26: 552-564. Published February 2014. DOI: https://doi.org/10.1105/tpc.113.115220. During the assembly of Supplemental Figure 7 and Figure 8, six lucif...
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ftzenodo:oai:zenodo.org:4956793 2023-06-06T11:58:21+02:00 FT TFL1 bimolecular luciferase complementation assays Weigel, Detlef Ho, William 2020-04-07 https://zenodo.org/record/4956793 https://doi.org/10.5061/dryad.h9w0vt4fh unknown doi:10.1105/tpc.113.115220 https://zenodo.org/communities/dryad https://zenodo.org/record/4956793 https://doi.org/10.5061/dryad.h9w0vt4fh oai:zenodo.org:4956793 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode Arabidopsis thaliana flowering protein-protein-interaction FT TFL1 TCP GRF 14-3-3 protein info:eu-repo/semantics/other dataset 2020 ftzenodo https://doi.org/10.5061/dryad.h9w0vt4fh10.1105/tpc.113.115220 2023-04-13T21:20:22Z CORRECTION Ho, W.W.H., and Weigel, D. (2014). Structural Features Determining Flower-Promoting Activity of Arabidopsis FLOWERING LOCUS T. Plant Cell 26: 552-564. Published February 2014. DOI: https://doi.org/10.1105/tpc.113.115220. During the assembly of Supplemental Figure 7 and Figure 8, six luciferase images of individual leaves were inadvertently duplicated. These images served to illustrate the strength of interactions between FT and TFL1 proteins and their mutant variants with GRF and TCP proteins, as measured qualitatively in split luciferase assays and in support of independent, quantitative yeast-two-hybrid experiments. This Dryad data set includes the original photographs of bimolecular luciferase complementation assays after Agrobacterium tumefaciens mediated transient transformation of N. benthamiana leaves. The associated key links the constructs tested for bimolecular luciferase complementation with the photographs. Funding provided by: Croucher FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001692Award Number: Funding provided by: Max-Planck-GesellschaftCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100004189Award Number: Bimolecular Luciferase Complementation Assays Nicotiana benthamiana was transiently transformed as described (Voinnet et al., 2003), and samples were analyzed 4 days after inoculation (Gou et al., 2011). Before measurement, leaves only infiltrated with luciferin (negative control) and leaves infiltrated with a 35S:LUC construct and luciferin (positive control) were used for calibrating the Orca 2-BT cooled CCD camera (Hamamatsu Photonics, Hamamatsu, Japan). Gou, J.Y., Felippes, F.F., Liu, C.J., Weigel, D., and Wang, J.W. (2011). Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor. Plant Cell 23, 1512-1522. Voinnet, O., Rivas, S., Mestre, P., and Baulcombe, D. (2003). An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato ... Dataset Orca Zenodo |
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Arabidopsis thaliana flowering protein-protein-interaction FT TFL1 TCP GRF 14-3-3 protein |
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Arabidopsis thaliana flowering protein-protein-interaction FT TFL1 TCP GRF 14-3-3 protein Weigel, Detlef Ho, William FT TFL1 bimolecular luciferase complementation assays |
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Arabidopsis thaliana flowering protein-protein-interaction FT TFL1 TCP GRF 14-3-3 protein |
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CORRECTION Ho, W.W.H., and Weigel, D. (2014). Structural Features Determining Flower-Promoting Activity of Arabidopsis FLOWERING LOCUS T. Plant Cell 26: 552-564. Published February 2014. DOI: https://doi.org/10.1105/tpc.113.115220. During the assembly of Supplemental Figure 7 and Figure 8, six luciferase images of individual leaves were inadvertently duplicated. These images served to illustrate the strength of interactions between FT and TFL1 proteins and their mutant variants with GRF and TCP proteins, as measured qualitatively in split luciferase assays and in support of independent, quantitative yeast-two-hybrid experiments. This Dryad data set includes the original photographs of bimolecular luciferase complementation assays after Agrobacterium tumefaciens mediated transient transformation of N. benthamiana leaves. The associated key links the constructs tested for bimolecular luciferase complementation with the photographs. Funding provided by: Croucher FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001692Award Number: Funding provided by: Max-Planck-GesellschaftCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100004189Award Number: Bimolecular Luciferase Complementation Assays Nicotiana benthamiana was transiently transformed as described (Voinnet et al., 2003), and samples were analyzed 4 days after inoculation (Gou et al., 2011). Before measurement, leaves only infiltrated with luciferin (negative control) and leaves infiltrated with a 35S:LUC construct and luciferin (positive control) were used for calibrating the Orca 2-BT cooled CCD camera (Hamamatsu Photonics, Hamamatsu, Japan). Gou, J.Y., Felippes, F.F., Liu, C.J., Weigel, D., and Wang, J.W. (2011). Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor. Plant Cell 23, 1512-1522. Voinnet, O., Rivas, S., Mestre, P., and Baulcombe, D. (2003). An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato ... |
format |
Dataset |
author |
Weigel, Detlef Ho, William |
author_facet |
Weigel, Detlef Ho, William |
author_sort |
Weigel, Detlef |
title |
FT TFL1 bimolecular luciferase complementation assays |
title_short |
FT TFL1 bimolecular luciferase complementation assays |
title_full |
FT TFL1 bimolecular luciferase complementation assays |
title_fullStr |
FT TFL1 bimolecular luciferase complementation assays |
title_full_unstemmed |
FT TFL1 bimolecular luciferase complementation assays |
title_sort |
ft tfl1 bimolecular luciferase complementation assays |
publishDate |
2020 |
url |
https://zenodo.org/record/4956793 https://doi.org/10.5061/dryad.h9w0vt4fh |
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Orca |
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Orca |
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doi:10.1105/tpc.113.115220 https://zenodo.org/communities/dryad https://zenodo.org/record/4956793 https://doi.org/10.5061/dryad.h9w0vt4fh oai:zenodo.org:4956793 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.h9w0vt4fh10.1105/tpc.113.115220 |
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1767966960162701312 |