Data from: The use of archived tags in retrospective genetic analysis of fish
Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populatio...
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ftzenodo:oai:zenodo.org:4949138 2024-09-09T19:29:49+00:00 Data from: The use of archived tags in retrospective genetic analysis of fish Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg 2013-12-05 https://doi.org/10.5061/dryad.sp0ks unknown Zenodo https://doi.org/10.1111/1755-0998.12211 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.sp0ks oai:zenodo.org:4949138 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode historical samples Atlantic cod temporal genetic analysis fish tags info:eu-repo/semantics/other 2013 ftzenodo https://doi.org/10.5061/dryad.sp0ks10.1111/1755-0998.12211 2024-07-26T08:16:54Z Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish. Individual genotypes Allelic profile of otolith and tag extracts for each of the four amplified microsatellite loci : Gmo-C18, PGmo38, Tch11 and Tch14.For each extract two PCR replicates were provided. Dryad.xlsx Other/Unknown Material atlantic cod greenlandic Zenodo |
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historical samples Atlantic cod temporal genetic analysis fish tags |
spellingShingle |
historical samples Atlantic cod temporal genetic analysis fish tags Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg Data from: The use of archived tags in retrospective genetic analysis of fish |
topic_facet |
historical samples Atlantic cod temporal genetic analysis fish tags |
description |
Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish. Individual genotypes Allelic profile of otolith and tag extracts for each of the four amplified microsatellite loci : Gmo-C18, PGmo38, Tch11 and Tch14.For each extract two PCR replicates were provided. Dryad.xlsx |
format |
Other/Unknown Material |
author |
Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg |
author_facet |
Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg |
author_sort |
Bonanomi, Sara |
title |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_short |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_full |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_fullStr |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_full_unstemmed |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_sort |
data from: the use of archived tags in retrospective genetic analysis of fish |
publisher |
Zenodo |
publishDate |
2013 |
url |
https://doi.org/10.5061/dryad.sp0ks |
genre |
atlantic cod greenlandic |
genre_facet |
atlantic cod greenlandic |
op_relation |
https://doi.org/10.1111/1755-0998.12211 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.sp0ks oai:zenodo.org:4949138 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.sp0ks10.1111/1755-0998.12211 |
_version_ |
1809898904317067264 |