Data from: Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus

• Main Authors: Chu, Nathaniel D., Kaluziak, Stefan T., Trussell, Geoffrey C., Vollmer, Steven V.
• Format: Other/Unknown Material
• Language: unknown
• Published: Zenodo 2014
• Subjects: Molluscs; Nucella lapillus; Holocene; North Atlantic
• Online Access: https://doi.org/10.5061/dryad.610dd

North Atlantic rocky intertidal species have been shaped by repeated glaciations and strong latitudinal temperature gradients, making them an excellent system to study postglacial phylogeography and thermal tolerance. Population genetics data from northwestern Atlantic species, however, often show patterns inconsistent with the prediction that high dispersal should generate weaker genetic structure among populations. Here we used next-generation sequencing restriction-associated DNA tags (RAD-seq) and a transcriptome assembled from RNA-seq data to analyze the genetic structure of northwestern Atlantic populations of the low-dispersal intertidal snail Nucella lapillus. Although previous studies in this region have detected almost no genetic structure in N. lapillus, our phylogenomic approach identified a well-supported split between northern and southern clades. By comparing RAD-seq data and our transcriptome assembly, we identified thousands of fixed single-nucleotide polymorphisms (SNPs) between these latitudinal clades that map to protein-coding genes, including genes associated with heat-stress tolerance. These fixed SNPs might represent loci under selection for different thermal regimes in the northwestern Atlantic. IUPACAlignmentLessThan4SNPsPhylipFormat This is a Phylip file alignment (not interleaved) of RAD-seq assembled data from pooled DNA samples from Nucella lapillus populations along the Northwestern Atlantic. For more information, please contact Nathaniel Chu ([e-mail]). RAD-tag data set in TSV format for northwestern Atlantic Nucella lapillus populations This is the easier to visualize data output (tsv format) from the STACKs assembly described in the paper. It contains all retained "stacks" including those that contain SNPs and those that have a single consensus sequence. Note that stacks with 4 or more SNPs were not used in the analyses. For more information, please contact [e-mail] NucellaRadTags.tsv RAD-tag depths in TSV format This file contains the depths of ...