Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin
Like many aquatic vertebrates, whales have blue-shifting spectral tuning substitutions in the dim-light visual pigment, rhodopsin, that are thought to increase photosensitivity in underwater environments. We have discovered that known spectral tuning substitutions also have surprising epistatic effe...
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ftzenodo:oai:zenodo.org:4944325 2023-06-06T11:56:07+02:00 Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin Dungan, Sarah Z. Chang, Belinda S. W. 2017-02-06 https://zenodo.org/record/4944325 https://doi.org/10.5061/dryad.5k0s6 unknown doi:10.1098/rspb.2016.2743 https://zenodo.org/communities/dryad https://zenodo.org/record/4944325 https://doi.org/10.5061/dryad.5k0s6 oai:zenodo.org:4944325 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode protein evolution epistasis evolution of protein structure-function Meta II stability Cetacea spectral tuning Orcinus orca info:eu-repo/semantics/other dataset 2017 ftzenodo https://doi.org/10.5061/dryad.5k0s610.1098/rspb.2016.2743 2023-04-13T21:21:25Z Like many aquatic vertebrates, whales have blue-shifting spectral tuning substitutions in the dim-light visual pigment, rhodopsin, that are thought to increase photosensitivity in underwater environments. We have discovered that known spectral tuning substitutions also have surprising epistatic effects on another function of rhodopsin, the kinetic rates associated with light-activated intermediates. By using absorbance spectroscopy and fluorescence-based retinal release assays on heterologously expressed rhodopsin, we assessed both spectral and kinetic differences between cetaceans (killer whale) and terrestrial outgroups (hippo, bovine). Mutation experiments revealed that killer whale rhodopsin is unusually resilient to pleiotropic effects on retinal release from key blue-shifting substitutions (D83N and A292S), largely due to a surprisingly specific epistatic interaction between D83N and the background residue, S299. Ancestral sequence reconstruction indicated that S299 is an ancestral residue that predates the evolution of blue-shifting substitutions at the origins of Cetacea. Based on these results, we hypothesize that intramolecular epistasis helped to conserve rhodopsin's kinetic properties while enabling blue-shifting spectral tuning substitutions as cetaceans adapted to aquatic environments. Trade-offs between different aspects of molecular function are rarely considered in protein evolution, but in cetacean and other vertebrate rhodopsins, may underlie multiple evolutionary scenarios for the selection of specific amino acid substitutions. Data for Dungan & Chang 2017, Proceedings BThe .zip archive contains two files: 1) A fasta alignment of the rhodopsin coding sequences used for ancestral sequence reconstruction and 2) an excel file with fluorescence and absorbance data seriesDunganChang2017_ProcB_Data.zip Dataset Killer Whale Orca Orcinus orca Killer whale Zenodo |
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op_collection_id |
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language |
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topic |
protein evolution epistasis evolution of protein structure-function Meta II stability Cetacea spectral tuning Orcinus orca |
spellingShingle |
protein evolution epistasis evolution of protein structure-function Meta II stability Cetacea spectral tuning Orcinus orca Dungan, Sarah Z. Chang, Belinda S. W. Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
topic_facet |
protein evolution epistasis evolution of protein structure-function Meta II stability Cetacea spectral tuning Orcinus orca |
description |
Like many aquatic vertebrates, whales have blue-shifting spectral tuning substitutions in the dim-light visual pigment, rhodopsin, that are thought to increase photosensitivity in underwater environments. We have discovered that known spectral tuning substitutions also have surprising epistatic effects on another function of rhodopsin, the kinetic rates associated with light-activated intermediates. By using absorbance spectroscopy and fluorescence-based retinal release assays on heterologously expressed rhodopsin, we assessed both spectral and kinetic differences between cetaceans (killer whale) and terrestrial outgroups (hippo, bovine). Mutation experiments revealed that killer whale rhodopsin is unusually resilient to pleiotropic effects on retinal release from key blue-shifting substitutions (D83N and A292S), largely due to a surprisingly specific epistatic interaction between D83N and the background residue, S299. Ancestral sequence reconstruction indicated that S299 is an ancestral residue that predates the evolution of blue-shifting substitutions at the origins of Cetacea. Based on these results, we hypothesize that intramolecular epistasis helped to conserve rhodopsin's kinetic properties while enabling blue-shifting spectral tuning substitutions as cetaceans adapted to aquatic environments. Trade-offs between different aspects of molecular function are rarely considered in protein evolution, but in cetacean and other vertebrate rhodopsins, may underlie multiple evolutionary scenarios for the selection of specific amino acid substitutions. Data for Dungan & Chang 2017, Proceedings BThe .zip archive contains two files: 1) A fasta alignment of the rhodopsin coding sequences used for ancestral sequence reconstruction and 2) an excel file with fluorescence and absorbance data seriesDunganChang2017_ProcB_Data.zip |
format |
Dataset |
author |
Dungan, Sarah Z. Chang, Belinda S. W. |
author_facet |
Dungan, Sarah Z. Chang, Belinda S. W. |
author_sort |
Dungan, Sarah Z. |
title |
Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
title_short |
Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
title_full |
Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
title_fullStr |
Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
title_full_unstemmed |
Data from: Epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
title_sort |
data from: epistatic interactions influence terrestrial-marine functional shifts in cetacean rhodopsin |
publishDate |
2017 |
url |
https://zenodo.org/record/4944325 https://doi.org/10.5061/dryad.5k0s6 |
genre |
Killer Whale Orca Orcinus orca Killer whale |
genre_facet |
Killer Whale Orca Orcinus orca Killer whale |
op_relation |
doi:10.1098/rspb.2016.2743 https://zenodo.org/communities/dryad https://zenodo.org/record/4944325 https://doi.org/10.5061/dryad.5k0s6 oai:zenodo.org:4944325 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.5k0s610.1098/rspb.2016.2743 |
_version_ |
1767963494565543936 |