Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF)
Recently, MALDI-TOF mass spectrometry has been used to reliably identify taxonomically difficult harpacticoid copepods from sediment samples. In agreement with former studies, a negative impact of short storage periods was stated. Other studies reported inferior mass spectra quality from samples fix...
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ftzenodo:oai:zenodo.org:4943866 2024-09-09T20:14:20+00:00 Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) Rossel, Sven Martínez Arbizu, Pedro 2019-04-13 https://doi.org/10.5061/dryad.1md2jq1 unknown Zenodo https://doi.org/10.3389/fmars.2018.00149 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.1md2jq1 oai:zenodo.org:4943866 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode meiofauna Harpacticus flexus Harpacticoida Ectinosomatidae spec MALDI-TOF MS meiobenthos fixation storage Enhydrosoma propinquum Microarthridion fallax Copepoda Proteome Fingerprint Microarthridion littorale info:eu-repo/semantics/other 2019 ftzenodo https://doi.org/10.5061/dryad.1md2jq110.3389/fmars.2018.00149 2024-07-25T08:27:51Z Recently, MALDI-TOF mass spectrometry has been used to reliably identify taxonomically difficult harpacticoid copepods from sediment samples. In agreement with former studies, a negative impact of short storage periods was stated. Other studies reported inferior mass spectra quality from samples fixated in varying ethanol concentrations. Therefore, sediment samples from a mudflat sampling site in the North Sea were stored under different temperature conditions to explore a possible storage effect. Samples were fixated with either 70% or 100% ethanol and specimens were measured using MALDI-TOF mass spectrometry after one, two, three, five, seven and 12 weeks. The changes in number of peaks per species and the ability to identify specimens based on mass spectra were analyzed quality measurements. We show that storage temperature had a major impact on data quality, as for some species a loss of up to 50% of mass peaks and an increase of failed measurements to over 70% was observed. However, the effect of different ethanol concentrations on data quality was negligible. Concluding from these results, storage of metazoan samples in general and, particularly, of sediment samples at low temperatures of around -25°C is recommended to receive high-quality mass spectra for specimen identification. Hellinger transformed MALDI-TOF MS intensity matrix of all studied specimens The dataset contains hellinger transformed MALDI-TOF MS data measured with a Microflex LT/SH System (Bruker Daltonics) with a range from 3,000 to 15,000 m/z from 2,316 harpacticoid copepods from the North Sea (see publication for details). The rows contain the measured species. The first column contains sample names, the second column contains species names, the third column contains measurement weeks, the fourth column contains ethanol concentrations and the fifth column contains storage temperature. The remaining columns contain the 1,220 m/z values as names and the intensities as values. Effects_of_sample_fixation_for_MALDI-TOF.csv Other/Unknown Material Copepods Harpacticus Zenodo |
institution |
Open Polar |
collection |
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op_collection_id |
ftzenodo |
language |
unknown |
topic |
meiofauna Harpacticus flexus Harpacticoida Ectinosomatidae spec MALDI-TOF MS meiobenthos fixation storage Enhydrosoma propinquum Microarthridion fallax Copepoda Proteome Fingerprint Microarthridion littorale |
spellingShingle |
meiofauna Harpacticus flexus Harpacticoida Ectinosomatidae spec MALDI-TOF MS meiobenthos fixation storage Enhydrosoma propinquum Microarthridion fallax Copepoda Proteome Fingerprint Microarthridion littorale Rossel, Sven Martínez Arbizu, Pedro Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) |
topic_facet |
meiofauna Harpacticus flexus Harpacticoida Ectinosomatidae spec MALDI-TOF MS meiobenthos fixation storage Enhydrosoma propinquum Microarthridion fallax Copepoda Proteome Fingerprint Microarthridion littorale |
description |
Recently, MALDI-TOF mass spectrometry has been used to reliably identify taxonomically difficult harpacticoid copepods from sediment samples. In agreement with former studies, a negative impact of short storage periods was stated. Other studies reported inferior mass spectra quality from samples fixated in varying ethanol concentrations. Therefore, sediment samples from a mudflat sampling site in the North Sea were stored under different temperature conditions to explore a possible storage effect. Samples were fixated with either 70% or 100% ethanol and specimens were measured using MALDI-TOF mass spectrometry after one, two, three, five, seven and 12 weeks. The changes in number of peaks per species and the ability to identify specimens based on mass spectra were analyzed quality measurements. We show that storage temperature had a major impact on data quality, as for some species a loss of up to 50% of mass peaks and an increase of failed measurements to over 70% was observed. However, the effect of different ethanol concentrations on data quality was negligible. Concluding from these results, storage of metazoan samples in general and, particularly, of sediment samples at low temperatures of around -25°C is recommended to receive high-quality mass spectra for specimen identification. Hellinger transformed MALDI-TOF MS intensity matrix of all studied specimens The dataset contains hellinger transformed MALDI-TOF MS data measured with a Microflex LT/SH System (Bruker Daltonics) with a range from 3,000 to 15,000 m/z from 2,316 harpacticoid copepods from the North Sea (see publication for details). The rows contain the measured species. The first column contains sample names, the second column contains species names, the third column contains measurement weeks, the fourth column contains ethanol concentrations and the fifth column contains storage temperature. The remaining columns contain the 1,220 m/z values as names and the intensities as values. Effects_of_sample_fixation_for_MALDI-TOF.csv |
format |
Other/Unknown Material |
author |
Rossel, Sven Martínez Arbizu, Pedro |
author_facet |
Rossel, Sven Martínez Arbizu, Pedro |
author_sort |
Rossel, Sven |
title |
Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) |
title_short |
Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) |
title_full |
Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) |
title_fullStr |
Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) |
title_full_unstemmed |
Data from: Effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (MALDI-TOF) |
title_sort |
data from: effects of sample fixation on specimen identification in biodiversity assemblies based on proteomic data (maldi-tof) |
publisher |
Zenodo |
publishDate |
2019 |
url |
https://doi.org/10.5061/dryad.1md2jq1 |
genre |
Copepods Harpacticus |
genre_facet |
Copepods Harpacticus |
op_relation |
https://doi.org/10.3389/fmars.2018.00149 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.1md2jq1 oai:zenodo.org:4943866 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.1md2jq110.3389/fmars.2018.00149 |
_version_ |
1809816017323425792 |