Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats
The Adélie penguin is the most important animal currently used for ecosystem monitoring in the Southern Ocean. The diet of this species is generally studied by visual analysis of stomach contents; or ratios of isotopes of carbon and nitrogen incorporated into the penguin from its food. There are sig...
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ftzenodo:oai:zenodo.org:4937784 2024-09-15T18:31:40+00:00 Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats Jarman, Simon N. McInnes, Julie C. Faux, Cassandra Polanowski, Andrea M. Marthick, James Deagle, Bruce E. Southwell, Colin Emmerson, Louise 2014-10-08 https://doi.org/10.5061/dryad.1rf7d unknown Zenodo https://doi.org/10.1371/journal.pone.0082227 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.1rf7d oai:zenodo.org:4937784 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode CCAMLR Ecosystem Monitoring scat analysis Euphausiidae Pygoscelis adeliae Adélie penguin Ion Torrent Amplicon Sequencing info:eu-repo/semantics/other 2014 ftzenodo https://doi.org/10.5061/dryad.1rf7d10.1371/journal.pone.0082227 2024-07-27T05:26:23Z The Adélie penguin is the most important animal currently used for ecosystem monitoring in the Southern Ocean. The diet of this species is generally studied by visual analysis of stomach contents; or ratios of isotopes of carbon and nitrogen incorporated into the penguin from its food. There are significant limitations to the information that can be gained from these methods. We evaluated population diet assessment by analysis of food DNA in scats as an alternative method for ecosystem monitoring with Adélie penguins as an indicator species. Scats were collected at four locations, three phases of the breeding cycle, and in four different years. A novel molecular diet assay and bioinformatics pipeline based on nuclear small subunit ribosomal RNA gene (SSU rDNA) sequencing was used to identify prey DNA in 389 scats. Analysis of the twelve population sample sets identified spatial and temporal dietary change in Adélie penguin population diet. Prey diversity was found to be greater than previously thought. Krill, fish, copepods and amphipods were the most important food groups, in general agreement with other Adélie penguin dietary studies based on hard part or stable isotope analysis. However, our DNA analysis estimated that a substantial portion of the diet was gelatinous groups such as jellyfish and comb jellies. A range of other prey not previously identified in the diet of this species were also discovered. The diverse prey identified by this DNA-based scat analysis confirms that the generalist feeding of Adélie penguins makes them a useful indicator species for prey community composition in the coastal zone of the Southern Ocean. Scat collection is a simple and non-invasive field sampling method that allows DNA-based estimation of prey community differences at many temporal and spatial scales and provides significant advantages over alternative diet analysis approaches. 1_AdelieDietPopulationResults 2_FASTQfiles 3_SampleKeyFiles 4_SSUdietPipeline Other/Unknown Material Pygoscelis adeliae Southern Ocean Copepods Zenodo |
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Open Polar |
collection |
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ftzenodo |
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unknown |
topic |
CCAMLR Ecosystem Monitoring scat analysis Euphausiidae Pygoscelis adeliae Adélie penguin Ion Torrent Amplicon Sequencing |
spellingShingle |
CCAMLR Ecosystem Monitoring scat analysis Euphausiidae Pygoscelis adeliae Adélie penguin Ion Torrent Amplicon Sequencing Jarman, Simon N. McInnes, Julie C. Faux, Cassandra Polanowski, Andrea M. Marthick, James Deagle, Bruce E. Southwell, Colin Emmerson, Louise Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats |
topic_facet |
CCAMLR Ecosystem Monitoring scat analysis Euphausiidae Pygoscelis adeliae Adélie penguin Ion Torrent Amplicon Sequencing |
description |
The Adélie penguin is the most important animal currently used for ecosystem monitoring in the Southern Ocean. The diet of this species is generally studied by visual analysis of stomach contents; or ratios of isotopes of carbon and nitrogen incorporated into the penguin from its food. There are significant limitations to the information that can be gained from these methods. We evaluated population diet assessment by analysis of food DNA in scats as an alternative method for ecosystem monitoring with Adélie penguins as an indicator species. Scats were collected at four locations, three phases of the breeding cycle, and in four different years. A novel molecular diet assay and bioinformatics pipeline based on nuclear small subunit ribosomal RNA gene (SSU rDNA) sequencing was used to identify prey DNA in 389 scats. Analysis of the twelve population sample sets identified spatial and temporal dietary change in Adélie penguin population diet. Prey diversity was found to be greater than previously thought. Krill, fish, copepods and amphipods were the most important food groups, in general agreement with other Adélie penguin dietary studies based on hard part or stable isotope analysis. However, our DNA analysis estimated that a substantial portion of the diet was gelatinous groups such as jellyfish and comb jellies. A range of other prey not previously identified in the diet of this species were also discovered. The diverse prey identified by this DNA-based scat analysis confirms that the generalist feeding of Adélie penguins makes them a useful indicator species for prey community composition in the coastal zone of the Southern Ocean. Scat collection is a simple and non-invasive field sampling method that allows DNA-based estimation of prey community differences at many temporal and spatial scales and provides significant advantages over alternative diet analysis approaches. 1_AdelieDietPopulationResults 2_FASTQfiles 3_SampleKeyFiles 4_SSUdietPipeline |
format |
Other/Unknown Material |
author |
Jarman, Simon N. McInnes, Julie C. Faux, Cassandra Polanowski, Andrea M. Marthick, James Deagle, Bruce E. Southwell, Colin Emmerson, Louise |
author_facet |
Jarman, Simon N. McInnes, Julie C. Faux, Cassandra Polanowski, Andrea M. Marthick, James Deagle, Bruce E. Southwell, Colin Emmerson, Louise |
author_sort |
Jarman, Simon N. |
title |
Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats |
title_short |
Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats |
title_full |
Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats |
title_fullStr |
Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats |
title_full_unstemmed |
Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats |
title_sort |
data from: adélie penguin population diet monitoring by analysis of food dna in scats |
publisher |
Zenodo |
publishDate |
2014 |
url |
https://doi.org/10.5061/dryad.1rf7d |
genre |
Pygoscelis adeliae Southern Ocean Copepods |
genre_facet |
Pygoscelis adeliae Southern Ocean Copepods |
op_relation |
https://doi.org/10.1371/journal.pone.0082227 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.1rf7d oai:zenodo.org:4937784 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.1rf7d10.1371/journal.pone.0082227 |
_version_ |
1810473424687988736 |