Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbor porpoise (Phocoena phocoena), on...
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ftzenodo:oai:zenodo.org:4936969 2024-09-15T18:30:25+00:00 Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda 2018-07-09 https://doi.org/10.5061/dryad.77h8fd8 unknown Zenodo https://doi.org/10.1098/rsos.180537 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.77h8fd8 oai:zenodo.org:4936969 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode Next Generation Sequencing porpoise Phocoena phocoena Stock cetacean info:eu-repo/semantics/other 2018 ftzenodo https://doi.org/10.5061/dryad.77h8fd810.1098/rsos.180537 2024-07-25T20:37:54Z Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbor porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries bycatch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. Phocoena_CR_sequences_Dryad_040418 Control region sequences resolved from Alaska harbor porpoise. Other/Unknown Material Phocoena phocoena Alaska Zenodo |
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collection |
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Next Generation Sequencing porpoise Phocoena phocoena Stock cetacean |
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Next Generation Sequencing porpoise Phocoena phocoena Stock cetacean Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
topic_facet |
Next Generation Sequencing porpoise Phocoena phocoena Stock cetacean |
description |
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbor porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries bycatch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. Phocoena_CR_sequences_Dryad_040418 Control region sequences resolved from Alaska harbor porpoise. |
format |
Other/Unknown Material |
author |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda |
author_facet |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda |
author_sort |
Parsons, Kim M. |
title |
Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_short |
Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full |
Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_fullStr |
Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full_unstemmed |
Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_sort |
data from: water, water everywhere: environmental dna can unlock population structure in elusive marine species |
publisher |
Zenodo |
publishDate |
2018 |
url |
https://doi.org/10.5061/dryad.77h8fd8 |
genre |
Phocoena phocoena Alaska |
genre_facet |
Phocoena phocoena Alaska |
op_relation |
https://doi.org/10.1098/rsos.180537 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.77h8fd8 oai:zenodo.org:4936969 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.77h8fd810.1098/rsos.180537 |
_version_ |
1810471886567505920 |