Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus
Many arthropods undergo a seasonal dormancy termed "diapause" to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others int...
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ftzenodo:oai:zenodo.org:4893627 2024-09-15T18:00:38+00:00 Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus Lenz, Petra H. Roncalli, Vittoria Cieslak, Matthew C. Tarrant, Ann M. Castelfranco, Ann M. Hartline, Daniel K. 2021-04-07 https://doi.org/10.5061/dryad.12jm63xw7 unknown Zenodo https://doi.org/10.1186/s12983-014-0091-8 https://doi.org/10.1093/icb/icw060 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.12jm63xw7 oai:zenodo.org:4893627 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode Calanus finmarchicus North Atlantic copepod life history info:eu-repo/semantics/other 2021 ftzenodo https://doi.org/10.5061/dryad.12jm63xw710.1186/s12983-014-0091-810.1093/icb/icw060 2024-07-26T13:16:35Z Many arthropods undergo a seasonal dormancy termed "diapause" to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others interrupt their development to enter diapause. It is unknown which, why and when individuals enter the diapause program. Transcriptomic data from copepods on known programs were analyzed using dimensionality reduction of gene expression and functional analyses to identify program-specific genes and biological processes. These analyses elucidated physiological differences and established protocols that distinguish between programs. Differences in gene expression were associated with maturation of individuals on the reproductive program, while those on the diapause program showed little change over time. Only two of six filters effectively separated copepods by developmental program. The first one included all genes annotated to RNA metabolism and this was confirmed using differential gene expression analysis. The second filter identified 54 differentially expressed genes that were consistently up-regulated in individuals on the diapause program in comparison with those on the reproductive program. Annotated to oogenesis, RNA metabolism and fatty acid biosynthesis, these genes are both indicators for diapause preparation and good candidates for functional studies. Funding provided by: National Science Foundation Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001 Award Number: OCE-1459235 Funding provided by: National Science Foundation Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001 Award Number: OCE-1756767 Funding provided by: National Science Foundation Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001 Award Number: OPP-1746087 Other/Unknown Material Calanus finmarchicus North Atlantic Copepods Zenodo |
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Calanus finmarchicus North Atlantic copepod life history |
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Calanus finmarchicus North Atlantic copepod life history Lenz, Petra H. Roncalli, Vittoria Cieslak, Matthew C. Tarrant, Ann M. Castelfranco, Ann M. Hartline, Daniel K. Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus |
topic_facet |
Calanus finmarchicus North Atlantic copepod life history |
description |
Many arthropods undergo a seasonal dormancy termed "diapause" to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others interrupt their development to enter diapause. It is unknown which, why and when individuals enter the diapause program. Transcriptomic data from copepods on known programs were analyzed using dimensionality reduction of gene expression and functional analyses to identify program-specific genes and biological processes. These analyses elucidated physiological differences and established protocols that distinguish between programs. Differences in gene expression were associated with maturation of individuals on the reproductive program, while those on the diapause program showed little change over time. Only two of six filters effectively separated copepods by developmental program. The first one included all genes annotated to RNA metabolism and this was confirmed using differential gene expression analysis. The second filter identified 54 differentially expressed genes that were consistently up-regulated in individuals on the diapause program in comparison with those on the reproductive program. Annotated to oogenesis, RNA metabolism and fatty acid biosynthesis, these genes are both indicators for diapause preparation and good candidates for functional studies. Funding provided by: National Science Foundation Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001 Award Number: OCE-1459235 Funding provided by: National Science Foundation Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001 Award Number: OCE-1756767 Funding provided by: National Science Foundation Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001 Award Number: OPP-1746087 |
format |
Other/Unknown Material |
author |
Lenz, Petra H. Roncalli, Vittoria Cieslak, Matthew C. Tarrant, Ann M. Castelfranco, Ann M. Hartline, Daniel K. |
author_facet |
Lenz, Petra H. Roncalli, Vittoria Cieslak, Matthew C. Tarrant, Ann M. Castelfranco, Ann M. Hartline, Daniel K. |
author_sort |
Lenz, Petra H. |
title |
Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus |
title_short |
Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus |
title_full |
Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus |
title_fullStr |
Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus |
title_full_unstemmed |
Diapause vs. reproductive programs: transcriptional phenotypes in Calanus finmarchicus |
title_sort |
diapause vs. reproductive programs: transcriptional phenotypes in calanus finmarchicus |
publisher |
Zenodo |
publishDate |
2021 |
url |
https://doi.org/10.5061/dryad.12jm63xw7 |
genre |
Calanus finmarchicus North Atlantic Copepods |
genre_facet |
Calanus finmarchicus North Atlantic Copepods |
op_relation |
https://doi.org/10.1186/s12983-014-0091-8 https://doi.org/10.1093/icb/icw060 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.12jm63xw7 oai:zenodo.org:4893627 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.12jm63xw710.1186/s12983-014-0091-810.1093/icb/icw060 |
_version_ |
1810437816323145728 |