Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast
Turbot (Scophthalmus maximus L., 1758) is a valuable commercial fish species classified as endangered. The conservation and sustainability of the turbot populations require knowledge of the population's genetic structure and constant monitoring of its biodiversity. The present study was perform...
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2021
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Online Access: | https://doi.org/10.3897/natureconservation.43.64195 |
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ftzenodo:oai:zenodo.org:4717198 2024-09-15T18:33:59+00:00 Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast Ivanova, Petya Dzhembekova, Nina Atanassov, Ivan Rusanov, Krasimir Raykov, Violin Zlateva, Ivelina Yankova, Maria Raev, Yordan Nikolov, Galin 2021-04-16 https://doi.org/10.3897/natureconservation.43.64195 unknown Pensoft Publishers https://doi.org/10.3897/natureconservation.43.64195.suppl1 https://zenodo.org/communities/biosyslit https://doi.org/10.3897/natureconservation.43.64195 oai:zenodo.org:4717198 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Nature Conservation, 43, 123-146, (2021-04-16) Animalia Chordata Actinopterygii Pleuronectiformes Scophthalmidae Scophthalmus Scophthalmus maximus Black Sea COIII CR microsatellite genotyping population structure turbot info:eu-repo/semantics/article 2021 ftzenodo https://doi.org/10.3897/natureconservation.43.6419510.3897/natureconservation.43.64195.suppl1 2024-07-26T18:41:46Z Turbot (Scophthalmus maximus L., 1758) is a valuable commercial fish species classified as endangered. The conservation and sustainability of the turbot populations require knowledge of the population's genetic structure and constant monitoring of its biodiversity. The present study was performed to evaluate the population structure of turbot along the Bulgarian Black Sea coast using seven pairs of microsatellites, two mitochondrial DNA (COIII and CR) and 23 morphological (15 morphometric and 8 meristic) markers. A total of 72 specimens at three locations were genotyped and 59 alleles were identified. The observed number of alleles of microsatellites was more than the effective number of alleles. The overall mean values of observed (Ho) and expected heterogeneity (He) were 0.638 and 0.685. A high rate of migration between turbot populations (overall mean of Nm = 17.484), with the maximum value (19.498) between Shabla and Nesebar locations, was observed. This result corresponded to the low level of genetic differentiation amongst these populations (overall mean Fst = 0.014), but there was no correlation between genetic and geographical distance. A high level of genetic diversity in the populations was also observed. The average Garza-Williamson M index value for all populations was low (0.359), suggesting a reduction in genetic variation due to a founder effect or a genetic bottleneck. Concerning mitochondrial DNA, a total number of 17 haplotypes for COIII and 41 haplotypes for CR were identified. The mitochondrial DNA control region showed patterns with high haplotype diversity and very low nucleotide diversity, indicating a significant number of closely-related haplotypes and suggesting that this population may have undergone a recent expansion. Tajima's D test and Fu's FS test suggested recent population growth. Pairwise Fst values were very low. The admixture and lack of genetic structuring found pointed to the populations analysed probably belonging to the same genetic unit. Therefore, a proper understanding ... Article in Journal/Newspaper Scophthalmus maximus Turbot Zenodo Nature Conservation 43 123 146 |
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Open Polar |
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op_collection_id |
ftzenodo |
language |
unknown |
topic |
Animalia Chordata Actinopterygii Pleuronectiformes Scophthalmidae Scophthalmus Scophthalmus maximus Black Sea COIII CR microsatellite genotyping population structure turbot |
spellingShingle |
Animalia Chordata Actinopterygii Pleuronectiformes Scophthalmidae Scophthalmus Scophthalmus maximus Black Sea COIII CR microsatellite genotyping population structure turbot Ivanova, Petya Dzhembekova, Nina Atanassov, Ivan Rusanov, Krasimir Raykov, Violin Zlateva, Ivelina Yankova, Maria Raev, Yordan Nikolov, Galin Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast |
topic_facet |
Animalia Chordata Actinopterygii Pleuronectiformes Scophthalmidae Scophthalmus Scophthalmus maximus Black Sea COIII CR microsatellite genotyping population structure turbot |
description |
Turbot (Scophthalmus maximus L., 1758) is a valuable commercial fish species classified as endangered. The conservation and sustainability of the turbot populations require knowledge of the population's genetic structure and constant monitoring of its biodiversity. The present study was performed to evaluate the population structure of turbot along the Bulgarian Black Sea coast using seven pairs of microsatellites, two mitochondrial DNA (COIII and CR) and 23 morphological (15 morphometric and 8 meristic) markers. A total of 72 specimens at three locations were genotyped and 59 alleles were identified. The observed number of alleles of microsatellites was more than the effective number of alleles. The overall mean values of observed (Ho) and expected heterogeneity (He) were 0.638 and 0.685. A high rate of migration between turbot populations (overall mean of Nm = 17.484), with the maximum value (19.498) between Shabla and Nesebar locations, was observed. This result corresponded to the low level of genetic differentiation amongst these populations (overall mean Fst = 0.014), but there was no correlation between genetic and geographical distance. A high level of genetic diversity in the populations was also observed. The average Garza-Williamson M index value for all populations was low (0.359), suggesting a reduction in genetic variation due to a founder effect or a genetic bottleneck. Concerning mitochondrial DNA, a total number of 17 haplotypes for COIII and 41 haplotypes for CR were identified. The mitochondrial DNA control region showed patterns with high haplotype diversity and very low nucleotide diversity, indicating a significant number of closely-related haplotypes and suggesting that this population may have undergone a recent expansion. Tajima's D test and Fu's FS test suggested recent population growth. Pairwise Fst values were very low. The admixture and lack of genetic structuring found pointed to the populations analysed probably belonging to the same genetic unit. Therefore, a proper understanding ... |
format |
Article in Journal/Newspaper |
author |
Ivanova, Petya Dzhembekova, Nina Atanassov, Ivan Rusanov, Krasimir Raykov, Violin Zlateva, Ivelina Yankova, Maria Raev, Yordan Nikolov, Galin |
author_facet |
Ivanova, Petya Dzhembekova, Nina Atanassov, Ivan Rusanov, Krasimir Raykov, Violin Zlateva, Ivelina Yankova, Maria Raev, Yordan Nikolov, Galin |
author_sort |
Ivanova, Petya |
title |
Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast |
title_short |
Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast |
title_full |
Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast |
title_fullStr |
Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast |
title_full_unstemmed |
Genetic diversity and morphological characterisation of three turbot (Scophthalmus maximus L., 1758) populations along the Bulgarian Black Sea coast |
title_sort |
genetic diversity and morphological characterisation of three turbot (scophthalmus maximus l., 1758) populations along the bulgarian black sea coast |
publisher |
Pensoft Publishers |
publishDate |
2021 |
url |
https://doi.org/10.3897/natureconservation.43.64195 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Nature Conservation, 43, 123-146, (2021-04-16) |
op_relation |
https://doi.org/10.3897/natureconservation.43.64195.suppl1 https://zenodo.org/communities/biosyslit https://doi.org/10.3897/natureconservation.43.64195 oai:zenodo.org:4717198 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.3897/natureconservation.43.6419510.3897/natureconservation.43.64195.suppl1 |
container_title |
Nature Conservation |
container_volume |
43 |
container_start_page |
123 |
op_container_end_page |
146 |
_version_ |
1810475722043555840 |