Raw data for predicting sample success for large-scale ancient DNA studies on marine mammals

• Main Authors: Keighley, Xénia, Tange Olsen, Morten
• Format: Other/Unknown Material
• Language: unknown
• Published: Zenodo 2021
• Subjects: North Atlantic; walrus*
• Online Access: https://doi.org/10.5061/dryad.tht76hdz0

In recent years, non-human ancient DNA studies have begun to focus on larger sample sizes and whole genomes, offering the potential to reveal exciting and hitherto unknown answers to ongoing biological and archaeological questions. However, one major limitation to the feasibility of such studies is the substantial financial and time investments still required during sample screening, due to uncertainty regarding successful sample selection. This study investigates the effect of a wide range of sample properties including latitude, sample age, skeletal element, collagen preservation, and context on endogenous content and DNA damage profiles for 317 ancient and historic pinniped samples collected from across the North Atlantic. Using generalised linear and mixed-effect models, we found that a range of factors affected DNA preservation within each of the species under consideration. The most important findings were that endogenous content varied significantly according to context, the type of skeletal element, the collagen content and collection year. There also appears to be an effect of the sample's geographic origin, with samples from the Arctic generally showing higher endogenous content and lower damage rates. Both latitude and sample age were found to have significant relationships with damage levels, but only for walrus samples. Sex, ontogenetic age and extraction material preparation were not found to have any significant relationship with DNA preservation. Overall, the skeletal element and sample context were found to be the most influential factors and should therefore be considered when selecting samples for large-scale ancient genome studies. Samples listed are all those included in the study, not only those that were successfully sequenced. Values for certain samples are not available if the sample was collected but did not provide a usable extraction. In such cases fields are filled with 'NOT SCREENED'. As this represents a large number of samples of different species, collected by different ...