Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations
Background: Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in thawing permafrost, little is known abou...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2021
|
Subjects: | |
Online Access: | https://zenodo.org/record/4407477 https://doi.org/10.25338/B8D33C |
id |
ftzenodo:oai:zenodo.org:4407477 |
---|---|
record_format |
openpolar |
spelling |
ftzenodo:oai:zenodo.org:4407477 2023-05-15T17:58:12+02:00 Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations ter Horst, Anneliek Santos-Medellín, Christian Sorensen, Jackson Zinke, Laura Wilson, Rachel Johnston, Eric Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven Hanson, Paul Chanton, Jeffrey Schadt, Christopher Kostka, Joel Emerson, Joanne 2021-12-01 https://zenodo.org/record/4407477 https://doi.org/10.25338/B8D33C unknown doi:10.1101/2020.12.15.422944 doi:10.1186/s40168-021-01156-0 https://zenodo.org/communities/dryad https://zenodo.org/record/4407477 https://doi.org/10.25338/B8D33C oai:zenodo.org:4407477 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode viral ecology viromics soil viruses Peat soil microbial ecology info:eu-repo/semantics/other dataset 2021 ftzenodo https://doi.org/10.25338/B8D33C10.1101/2020.12.15.42294410.1186/s40168-021-01156-0 2023-03-11T00:22:09Z Background: Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in thawing permafrost, little is known about viruses in other peatlands. More generally, soil viral biogeography and its potential drivers are poorly understood at both local and global scales. Here, 87 metagenomes and five viral size-fraction metagenomes (viromes) from a boreal peatland in Northern Minnesota (from the SPRUCE whole-ecosystem warming experiment and surrounding bog) were analyzed for dsDNA viral community ecological patterns, and the recovered viral populations (vOTUs) were compared to a database of 261,799 vOTUs from diverse ecosystems. Results: Within the SPRUCE experiment, viral community composition was significantly correlated with peat depth, soil moisture, and carbon chemistry, including CH4 and CO2 concentrations, but not with temperature during the first two years of warming treatments. Peat vOTUs with aquatic-like signatures (shared predicted protein content with marine and/or freshwater vOTUs) were significantly enriched in surface, more waterlogged depths. Predicted host ranges for SPRUCE vOTUs were relatively narrow, generally involving a single bacterial host or two hosts from the same genus. Of the 4,326 vOTUs recovered from SPRUCE peat, 164 were previously detected in other soils, mostly peatlands. None of the previously identified 202,372 marine and freshwater vOTUs were detected in SPRUCE peat, but 1.9% of 78,203 genus-level viral clusters (VCs) were shared between soil and aquatic environments. On a per-sample basis, vOTU recovery was 32-fold higher from viromes compared to total metagenomes. Conclusions: Results suggest strong viral species boundaries between terrestrial and aquatic ecosystems and to some extent between peat and other soils, with differences less pronounced at the genus level. The significant enrichment of ... Dataset permafrost Zenodo |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
viral ecology viromics soil viruses Peat soil microbial ecology |
spellingShingle |
viral ecology viromics soil viruses Peat soil microbial ecology ter Horst, Anneliek Santos-Medellín, Christian Sorensen, Jackson Zinke, Laura Wilson, Rachel Johnston, Eric Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven Hanson, Paul Chanton, Jeffrey Schadt, Christopher Kostka, Joel Emerson, Joanne Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
topic_facet |
viral ecology viromics soil viruses Peat soil microbial ecology |
description |
Background: Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in thawing permafrost, little is known about viruses in other peatlands. More generally, soil viral biogeography and its potential drivers are poorly understood at both local and global scales. Here, 87 metagenomes and five viral size-fraction metagenomes (viromes) from a boreal peatland in Northern Minnesota (from the SPRUCE whole-ecosystem warming experiment and surrounding bog) were analyzed for dsDNA viral community ecological patterns, and the recovered viral populations (vOTUs) were compared to a database of 261,799 vOTUs from diverse ecosystems. Results: Within the SPRUCE experiment, viral community composition was significantly correlated with peat depth, soil moisture, and carbon chemistry, including CH4 and CO2 concentrations, but not with temperature during the first two years of warming treatments. Peat vOTUs with aquatic-like signatures (shared predicted protein content with marine and/or freshwater vOTUs) were significantly enriched in surface, more waterlogged depths. Predicted host ranges for SPRUCE vOTUs were relatively narrow, generally involving a single bacterial host or two hosts from the same genus. Of the 4,326 vOTUs recovered from SPRUCE peat, 164 were previously detected in other soils, mostly peatlands. None of the previously identified 202,372 marine and freshwater vOTUs were detected in SPRUCE peat, but 1.9% of 78,203 genus-level viral clusters (VCs) were shared between soil and aquatic environments. On a per-sample basis, vOTU recovery was 32-fold higher from viromes compared to total metagenomes. Conclusions: Results suggest strong viral species boundaries between terrestrial and aquatic ecosystems and to some extent between peat and other soils, with differences less pronounced at the genus level. The significant enrichment of ... |
format |
Dataset |
author |
ter Horst, Anneliek Santos-Medellín, Christian Sorensen, Jackson Zinke, Laura Wilson, Rachel Johnston, Eric Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven Hanson, Paul Chanton, Jeffrey Schadt, Christopher Kostka, Joel Emerson, Joanne |
author_facet |
ter Horst, Anneliek Santos-Medellín, Christian Sorensen, Jackson Zinke, Laura Wilson, Rachel Johnston, Eric Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven Hanson, Paul Chanton, Jeffrey Schadt, Christopher Kostka, Joel Emerson, Joanne |
author_sort |
ter Horst, Anneliek |
title |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_short |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_full |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_fullStr |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_full_unstemmed |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_sort |
minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
publishDate |
2021 |
url |
https://zenodo.org/record/4407477 https://doi.org/10.25338/B8D33C |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
doi:10.1101/2020.12.15.422944 doi:10.1186/s40168-021-01156-0 https://zenodo.org/communities/dryad https://zenodo.org/record/4407477 https://doi.org/10.25338/B8D33C oai:zenodo.org:4407477 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.25338/B8D33C10.1101/2020.12.15.42294410.1186/s40168-021-01156-0 |
_version_ |
1766166765401800704 |