In situ determination of Si, N, and P utilization by the demosponge Tethya citrina: A benthic-chamber approach

• Published in: PLOS ONE
• Main Authors: López-Acosta, María, Leynaert, Aude, Chavaud, Laurent, Amice, Erwan, Bihannic, Isabelle, Le Bec, Thierry, Maldonado, Manuel
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2020
• Subjects: NItrates; Nitrites; Phosphates; Oxygen; Sponges; Sea water; Habitats; Bacteria; European Union (EU); dissolved silicon; DSi; Conpsuntion rates; inorganic nutrients; Horizon 2020; Grant Agreement No 679849; Deep-sea Sponge Grounds Ecosystems of the North Atlantic: an integrated approach towards their preservation and sustainable exploitation; North Atlantic
• Online Access: https://doi.org/10.1371/journal.pone.0218787

ABSTRACT Sponges consume dissolved silicon (DSi) to build their skeletons. Few studies have attempted to quantify DSi utilization by these organisms and all available determinations come from laboratory measurements. Here we measured DSi consumption rates of the sponge Tethya citrina in its natural habitat, conducting 24h incubations in benthic chambers. Sponges consumed DSi at an average rate of 0.046±0.018μmol h -1 mL -1 when DSi avail- ability in its habitat was 8.3±1.8μM. Such DSi consumption rates significantly matched the values predicted by a kinetic model elsewhere developed previously for this species through laboratory incubations. These results support the use of laboratory incubations as a suitable approach to learn about DSi consumption. During the field incubations, utilization of other dissolved inorganic nutrients by this low-microbial-abundance (LMA) sponge was also mea- sured. The sponges were net sources of ammonium (-0.043±0.031μmol h-1mL -1 ), nitrate (-0.063±0.031μmol h -1 mL -1 ), nitrite (-0.007±0.003μmol h -1 mL -1 ), and phosphate (-0.004±0.005μmol h -1 mL -1 ), in agreement with the general pattern in other LMA species. The detected effluxes were among the lowest reported for sponges, which agreed with the low respiration rates characterizing this species (0.35±0.11μmol-O 2 h -1 mL -1 ). Despite rela- tively low flux, the dense population of T.citrina modifies the availability of dissolved inor- ganic nutrients in the demersal water of its habitat, contributing up to 14% of nitrate and nitrite stocks. Through these effects, the bottom layer contacting the benthic communities where siliceous LMA sponges abound can be partially depleted in DSi, but can benefit from inputs of N and P dissolved inorganic nutrients that are critical to primary producers. ACKNOWLEDGMENTS Authors thank Gérard Thouzeau and Eric Duvielbourg for technical assistance during fieldwork preparation and Valérie Coquille for help with N and P analysis. Jacques Clavier is thanked for his comments that help to design ...