Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon
A major goal in biology is to understand how evolution shapes variation in individual life histories. Genome-wide association studies have been successful in uncovering genome regions linked with traits underlying life history variation in a range of species. However, lack of functional studies of t...
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ftzenodo:oai:zenodo.org:4002009 2023-05-15T15:32:03+02:00 Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon Verta, Jukka-Pekka Debes, Paul Piavchenko, Nikolai Ruokolainen, Annukka Ovaskainen, Outi Moustakas-Verho, Jacqueline Tillanen, Seija Parre, Noora Aykanat, Tutku Erkinaro, Jaakko Primmer, Craig 2020-08-26 https://zenodo.org/record/4002009 https://doi.org/10.5061/dryad.k6djh9w4w unknown doi:10.1371/journal.pgen.1009055 https://zenodo.org/communities/dryad https://zenodo.org/record/4002009 https://doi.org/10.5061/dryad.k6djh9w4w oai:zenodo.org:4002009 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode Salmo salar digital droplet PCR allele-specific expression gene expression MCMCglmm info:eu-repo/semantics/other dataset 2020 ftzenodo https://doi.org/10.5061/dryad.k6djh9w4w10.1371/journal.pgen.1009055 2023-03-10T15:47:41Z A major goal in biology is to understand how evolution shapes variation in individual life histories. Genome-wide association studies have been successful in uncovering genome regions linked with traits underlying life history variation in a range of species. However, lack of functional studies of the discovered genotype-phenotype associations severely restrains our understanding how alternative life history traits evolved and are mediated at the molecular level. Here, we report a cis-regulatory mechanism whereby expression of alternative isoforms of the transcription co-factor vestigial-like 3 (vgll3) associate with variation in a key life history trait, age at maturity, in Atlantic salmon (Salmo salar). Using a common-garden experiment, we first show that vgll3 genotype associates with puberty timing in one-year-old salmon males. By way of temporal sampling of vgll3 expression in ten tissues across the first year of salmon development, we identify a pubertal transition in vgll3 expression where maturation coincided with a 66% reduction in testicular vgll3 expression. The late maturation allele was not only associated with a tendency to delay puberty, but also with expression of a rare transcript isoform of vgll3 pre-puberty. By comparing absolute vgll3 mRNA copies in heterozygotes we show that the expression difference between the earlyand late maturity alleles is largely cis-regulatory. We propose a model whereby expression of a rare isoform from the late allele shifts the liability of its carriers towards delaying puberty. These results exemplify the potential importance of regulatory differences as a mechanism for the evolution of life history traits. Analyses as per the associated article can be reproduced with the R MarkDown scripts provided within this accession. Funding provided by: European Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000781Award Number: 742312Funding provided by: Academy of FinlandCrossref Funder Registry ID: ... Dataset Atlantic salmon Salmo salar Zenodo |
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Salmo salar digital droplet PCR allele-specific expression gene expression MCMCglmm |
spellingShingle |
Salmo salar digital droplet PCR allele-specific expression gene expression MCMCglmm Verta, Jukka-Pekka Debes, Paul Piavchenko, Nikolai Ruokolainen, Annukka Ovaskainen, Outi Moustakas-Verho, Jacqueline Tillanen, Seija Parre, Noora Aykanat, Tutku Erkinaro, Jaakko Primmer, Craig Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon |
topic_facet |
Salmo salar digital droplet PCR allele-specific expression gene expression MCMCglmm |
description |
A major goal in biology is to understand how evolution shapes variation in individual life histories. Genome-wide association studies have been successful in uncovering genome regions linked with traits underlying life history variation in a range of species. However, lack of functional studies of the discovered genotype-phenotype associations severely restrains our understanding how alternative life history traits evolved and are mediated at the molecular level. Here, we report a cis-regulatory mechanism whereby expression of alternative isoforms of the transcription co-factor vestigial-like 3 (vgll3) associate with variation in a key life history trait, age at maturity, in Atlantic salmon (Salmo salar). Using a common-garden experiment, we first show that vgll3 genotype associates with puberty timing in one-year-old salmon males. By way of temporal sampling of vgll3 expression in ten tissues across the first year of salmon development, we identify a pubertal transition in vgll3 expression where maturation coincided with a 66% reduction in testicular vgll3 expression. The late maturation allele was not only associated with a tendency to delay puberty, but also with expression of a rare transcript isoform of vgll3 pre-puberty. By comparing absolute vgll3 mRNA copies in heterozygotes we show that the expression difference between the earlyand late maturity alleles is largely cis-regulatory. We propose a model whereby expression of a rare isoform from the late allele shifts the liability of its carriers towards delaying puberty. These results exemplify the potential importance of regulatory differences as a mechanism for the evolution of life history traits. Analyses as per the associated article can be reproduced with the R MarkDown scripts provided within this accession. Funding provided by: European Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000781Award Number: 742312Funding provided by: Academy of FinlandCrossref Funder Registry ID: ... |
format |
Dataset |
author |
Verta, Jukka-Pekka Debes, Paul Piavchenko, Nikolai Ruokolainen, Annukka Ovaskainen, Outi Moustakas-Verho, Jacqueline Tillanen, Seija Parre, Noora Aykanat, Tutku Erkinaro, Jaakko Primmer, Craig |
author_facet |
Verta, Jukka-Pekka Debes, Paul Piavchenko, Nikolai Ruokolainen, Annukka Ovaskainen, Outi Moustakas-Verho, Jacqueline Tillanen, Seija Parre, Noora Aykanat, Tutku Erkinaro, Jaakko Primmer, Craig |
author_sort |
Verta, Jukka-Pekka |
title |
Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon |
title_short |
Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon |
title_full |
Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon |
title_fullStr |
Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon |
title_full_unstemmed |
Data from: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon |
title_sort |
data from: cis-regulatory differences in isoform expression associate with life history strategy variation in atlantic salmon |
publishDate |
2020 |
url |
https://zenodo.org/record/4002009 https://doi.org/10.5061/dryad.k6djh9w4w |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.1371/journal.pgen.1009055 https://zenodo.org/communities/dryad https://zenodo.org/record/4002009 https://doi.org/10.5061/dryad.k6djh9w4w oai:zenodo.org:4002009 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.k6djh9w4w10.1371/journal.pgen.1009055 |
_version_ |
1766362560273055744 |