Amplicon sequence variants by sample table from Antarctic methane seeps

• Main Author: Thurber, Andrew
• Format: Dataset
• Language: unknown
• Published: 2020
• Subjects: Antarctic; Ross Sea; McMurdo Sound; McMurdo Station; Turtle Rocks; Antarc*; Antarctica
• Online Access: https://zenodo.org/record/3962768; https://doi.org/10.5061/dryad.0zpc866vh

Antarctica is estimated to contain as much as a quarter of earth's marine methane, however we have not discovered an active Antarctic methane seep limiting our understanding of the methane cycle. In 2011, an expansive (70m x 1m) microbial mat formed at 10m water depth in the Ross Sea, Antarctica and we carried out 16S rRNA gene analysis on samples collected one year and five years after the methane seep formed. The data set attached is the resulting Amplicon Sequence Variant table by sample that we used to track the community composition change during this time and in comparison to other sampling in the McMurdo sound region. Funding provided by: National Science FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000001Award Number: 1642570Funding provided by: National Science FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000001Award Number: 1103428 To identify the microbial community of the Cinder Cones Seep (CCS; 77° 47.998' S 166° 40.241' E), sediment cores were collected using SCUBA. Sampling points were randomly distributed within patches of white, putative sulfur-oxidizing filamentous bacteria in 2012 (n=3) and 2016 (n=12). Cores were also collected within 2m of the seep (called Reference; n=4) and at Control locations that were downslope, away from seep influence at Cinder Cones and 2 additional sites within McMurdo sound ('Jetty': 77° 51.101' S 166° 39.933' E and 'Turtle Rocks': 77° 44.615' S 166° 46.297' E; n= 3 at all control sites which were sampled at 20m water depth). A shallow linear microbial mat was also sampled, however with a single core so it is not included in quantitative statistical analysis. Cores were transported at in situ temp (-1.8oC) to McMurdo Station and sliced vertically (intervals given in Figure 4) and the sediment was frozen at -80oC for microbial characterization. A subset of cores had sequential 3cm deep subcores taken vertically for methane analysis and preserved as described below. DNA was extracted from between 0.25 and 0.5 grams ...