Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis
This is the accepted manuscript of the paper Javana Iman Yousefi, Pagnotta Mario Augusto, D΄Ovidio Renato. 2019. Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis. Eurasian Chemical Communications, 2(4), 516-535. http://www.echemcom.com/arti...
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ftzenodo:oai:zenodo.org:3824334 2023-05-15T18:13:30+02:00 Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis Javana, Iman Yousefi Pagnotta, Mario Augusto D'Ovidio, Renato 2019-12-20 https://zenodo.org/record/3824334 https://doi.org/10.33945/SAMI/ECC.2020.4.9 eng eng https://zenodo.org/communities/unitus https://zenodo.org/record/3824334 https://doi.org/10.33945/SAMI/ECC.2020.4.9 oai:zenodo.org:3824334 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode Genome SSR wheat tetraploid info:eu-repo/semantics/article publication-article 2019 ftzenodo https://doi.org/10.33945/SAMI/ECC.2020.4.9 2023-03-10T21:50:45Z This is the accepted manuscript of the paper Javana Iman Yousefi, Pagnotta Mario Augusto, D΄Ovidio Renato. 2019. Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis. Eurasian Chemical Communications, 2(4), 516-535. http://www.echemcom.com/article_99343.html Doi:10.33945/SAMI/ECC.2020.4.9 Abstract In this work, we studied the distribution of molecular markers in the chromosomes of tetraploid wheat. This distribution was drawn through the 192 line came from based on a cross between two durum wheat genotypes. The first parental line was a Triticum turgidum ssp. durum (Desf.). The second parental line was a durum wheat genotype derived from a cross between the Triticum turgidum ssp. Durum, (Omrabi 5). One hundred ninety-two F8 recombinant inbred lines (RILs) derived from the above mentioned cross by single-seed descent. A total of 254 markers were analyzed, including 216 microsatellites and 38 SNPs markers. Linkage analysis defined 14 linkage groups. Most markers (57.2%) were found to be located to the A genome, with an average of 12 markers per chromosome. The remaining (42.7%) were located to the B genome. To construct a stabilized (skeleton) map, markers interfering with map stability were removed. Efficient user-friendly methods for mapping plant genomes were highly desirable for the studies marker-assisted selection. SSR (microsatellite) markers are user-friendly and efficient in detecting polymorphism, but they detect few loci. The skeleton map consisted of 100 markers with a total length of 3170.29 cM and an average distance of 31.7 cM between adjacent markers. Majority of the markers showed a statistical significantly Mendelian segregation with 1:1 ratio (α=0.01). The highest percentage of markers was similar with the first parental. This SSR and SNP markers revealed a high proportion of clustering, which may be indicative of gene-rich regions. Some of the SSR, SNP markers were distributed for the first time on the current work. This project provided a ... Article in Journal/Newspaper sami Zenodo Augusto ENVELOPE(-61.613,-61.613,-64.054,-64.054) Eurasian Chemical Communications 2 4 516 535 |
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Genome SSR wheat tetraploid |
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Genome SSR wheat tetraploid Javana, Iman Yousefi Pagnotta, Mario Augusto D'Ovidio, Renato Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis |
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Genome SSR wheat tetraploid |
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This is the accepted manuscript of the paper Javana Iman Yousefi, Pagnotta Mario Augusto, D΄Ovidio Renato. 2019. Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis. Eurasian Chemical Communications, 2(4), 516-535. http://www.echemcom.com/article_99343.html Doi:10.33945/SAMI/ECC.2020.4.9 Abstract In this work, we studied the distribution of molecular markers in the chromosomes of tetraploid wheat. This distribution was drawn through the 192 line came from based on a cross between two durum wheat genotypes. The first parental line was a Triticum turgidum ssp. durum (Desf.). The second parental line was a durum wheat genotype derived from a cross between the Triticum turgidum ssp. Durum, (Omrabi 5). One hundred ninety-two F8 recombinant inbred lines (RILs) derived from the above mentioned cross by single-seed descent. A total of 254 markers were analyzed, including 216 microsatellites and 38 SNPs markers. Linkage analysis defined 14 linkage groups. Most markers (57.2%) were found to be located to the A genome, with an average of 12 markers per chromosome. The remaining (42.7%) were located to the B genome. To construct a stabilized (skeleton) map, markers interfering with map stability were removed. Efficient user-friendly methods for mapping plant genomes were highly desirable for the studies marker-assisted selection. SSR (microsatellite) markers are user-friendly and efficient in detecting polymorphism, but they detect few loci. The skeleton map consisted of 100 markers with a total length of 3170.29 cM and an average distance of 31.7 cM between adjacent markers. Majority of the markers showed a statistical significantly Mendelian segregation with 1:1 ratio (α=0.01). The highest percentage of markers was similar with the first parental. This SSR and SNP markers revealed a high proportion of clustering, which may be indicative of gene-rich regions. Some of the SSR, SNP markers were distributed for the first time on the current work. This project provided a ... |
format |
Article in Journal/Newspaper |
author |
Javana, Iman Yousefi Pagnotta, Mario Augusto D'Ovidio, Renato |
author_facet |
Javana, Iman Yousefi Pagnotta, Mario Augusto D'Ovidio, Renato |
author_sort |
Javana, Iman Yousefi |
title |
Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis |
title_short |
Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis |
title_full |
Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis |
title_fullStr |
Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis |
title_full_unstemmed |
Identifying profiles of SSR and SNP markers in cultivars of tetraploid wheat: physical and chemical analysis |
title_sort |
identifying profiles of ssr and snp markers in cultivars of tetraploid wheat: physical and chemical analysis |
publishDate |
2019 |
url |
https://zenodo.org/record/3824334 https://doi.org/10.33945/SAMI/ECC.2020.4.9 |
long_lat |
ENVELOPE(-61.613,-61.613,-64.054,-64.054) |
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Augusto |
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Augusto |
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sami |
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sami |
op_relation |
https://zenodo.org/communities/unitus https://zenodo.org/record/3824334 https://doi.org/10.33945/SAMI/ECC.2020.4.9 oai:zenodo.org:3824334 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.33945/SAMI/ECC.2020.4.9 |
container_title |
Eurasian Chemical Communications |
container_volume |
2 |
container_issue |
4 |
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516 |
op_container_end_page |
535 |
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1766186051474292736 |