Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology
Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be ma...
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ftzenodo:oai:zenodo.org:2665775 2023-05-15T13:53:13+02:00 Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin 2014-08-18 https://zenodo.org/record/2665775 https://doi.org/10.5281/zenodo.2665775 eng eng doi:10.5281/zenodo.2665774 https://zenodo.org/communities/hsr https://zenodo.org/record/2665775 https://doi.org/10.5281/zenodo.2665775 oai:zenodo.org:2665775 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode International Journal of Chemical and Molecular Engineering 8.0(8) info:eu-repo/semantics/article publication-article 2014 ftzenodo https://doi.org/10.5281/zenodo.266577510.5281/zenodo.2665774 2023-03-10T22:44:06Z Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be made by producing of more lipophilic derivatives. In this study, a preliminary investigation of lipase-catalyzed trans-esterification reaction of ethyl ferulate and olive oil was investigated. The reaction was catalyzed by immobilized lipase from Candida antarctica (Novozym 435), to produce ferulate ester, a sunscreen agent. A statistical approach of Response surface methodology (RSM) was used to evaluate the interactive effects of reaction temperature (40-80°C), reaction time (4-12 hours), and amount of enzyme (0.1-0.5 g). The optimum conditions derived via RSM were reaction temperature 60°C, reaction time 2.34 hours, and amount of enzyme 0.3 g. The actual experimental yield was 59.6% ferulate ester under optimum condition, which compared well to the maximum predicted value of 58.0%. Article in Journal/Newspaper Antarc* Antarctica Zenodo |
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English |
description |
Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be made by producing of more lipophilic derivatives. In this study, a preliminary investigation of lipase-catalyzed trans-esterification reaction of ethyl ferulate and olive oil was investigated. The reaction was catalyzed by immobilized lipase from Candida antarctica (Novozym 435), to produce ferulate ester, a sunscreen agent. A statistical approach of Response surface methodology (RSM) was used to evaluate the interactive effects of reaction temperature (40-80°C), reaction time (4-12 hours), and amount of enzyme (0.1-0.5 g). The optimum conditions derived via RSM were reaction temperature 60°C, reaction time 2.34 hours, and amount of enzyme 0.3 g. The actual experimental yield was 59.6% ferulate ester under optimum condition, which compared well to the maximum predicted value of 58.0%. |
format |
Article in Journal/Newspaper |
author |
S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin |
spellingShingle |
S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
author_facet |
S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin |
author_sort |
S. Mat Radzi |
title |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_short |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_full |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_fullStr |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_full_unstemmed |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_sort |
enzymatic synthesis of olive-based ferulate esters: optimization by response surface methodology |
publishDate |
2014 |
url |
https://zenodo.org/record/2665775 https://doi.org/10.5281/zenodo.2665775 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
International Journal of Chemical and Molecular Engineering 8.0(8) |
op_relation |
doi:10.5281/zenodo.2665774 https://zenodo.org/communities/hsr https://zenodo.org/record/2665775 https://doi.org/10.5281/zenodo.2665775 oai:zenodo.org:2665775 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.266577510.5281/zenodo.2665774 |
_version_ |
1766258194575785984 |