The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges
As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of t...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Zenodo
2016
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2016.01781 |
id |
ftzenodo:oai:zenodo.org:196262 |
---|---|
record_format |
openpolar |
spelling |
ftzenodo:oai:zenodo.org:196262 2024-09-09T19:23:21+00:00 The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges Hestetun, Jon T. Dahle, Håkon Jørgensen, Steffen L. Olsen, Bernt R. Rapp, Hans T. 2016-11-09 https://doi.org/10.3389/fmicb.2016.01781 unknown Zenodo https://doi.org/10.3389/fmicb.2016.01781 https://zenodo.org/communities/sponges https://zenodo.org/communities/eu oai:zenodo.org:196262 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Frontiers in Microbiology, 7, 14, (2016-11-09) Porifera Cladorhizidae Barbados Arctic Mid-Ocean Ridge hydrothermal vent methane seep isotope Cladorhiza methanophila European Union Horizon 2020 Grant Agreement No 679849 Deep-sea Sponge Grounds Ecosystems of the North Atlantic an integrated approach towards their preservation and sustainable exploitation SponGES info:eu-repo/semantics/article 2016 ftzenodo https://doi.org/10.3389/fmicb.2016.01781 2024-07-27T07:29:09Z As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism, and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae, and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ 13 C and δ 15 N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5 to 10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ 13 C and δ 15 N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, ... Article in Journal/Newspaper Arctic Jan Mayen North Atlantic Zenodo Arctic Jan Mayen Frontiers in Microbiology 7 |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
Porifera Cladorhizidae Barbados Arctic Mid-Ocean Ridge hydrothermal vent methane seep isotope Cladorhiza methanophila European Union Horizon 2020 Grant Agreement No 679849 Deep-sea Sponge Grounds Ecosystems of the North Atlantic an integrated approach towards their preservation and sustainable exploitation SponGES |
spellingShingle |
Porifera Cladorhizidae Barbados Arctic Mid-Ocean Ridge hydrothermal vent methane seep isotope Cladorhiza methanophila European Union Horizon 2020 Grant Agreement No 679849 Deep-sea Sponge Grounds Ecosystems of the North Atlantic an integrated approach towards their preservation and sustainable exploitation SponGES Hestetun, Jon T. Dahle, Håkon Jørgensen, Steffen L. Olsen, Bernt R. Rapp, Hans T. The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
topic_facet |
Porifera Cladorhizidae Barbados Arctic Mid-Ocean Ridge hydrothermal vent methane seep isotope Cladorhiza methanophila European Union Horizon 2020 Grant Agreement No 679849 Deep-sea Sponge Grounds Ecosystems of the North Atlantic an integrated approach towards their preservation and sustainable exploitation SponGES |
description |
As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism, and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae, and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ 13 C and δ 15 N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5 to 10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ 13 C and δ 15 N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, ... |
format |
Article in Journal/Newspaper |
author |
Hestetun, Jon T. Dahle, Håkon Jørgensen, Steffen L. Olsen, Bernt R. Rapp, Hans T. |
author_facet |
Hestetun, Jon T. Dahle, Håkon Jørgensen, Steffen L. Olsen, Bernt R. Rapp, Hans T. |
author_sort |
Hestetun, Jon T. |
title |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_short |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_full |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_fullStr |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_full_unstemmed |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_sort |
microbiome and occurrence of methanotrophy in carnivorous sponges |
publisher |
Zenodo |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmicb.2016.01781 |
geographic |
Arctic Jan Mayen |
geographic_facet |
Arctic Jan Mayen |
genre |
Arctic Jan Mayen North Atlantic |
genre_facet |
Arctic Jan Mayen North Atlantic |
op_source |
Frontiers in Microbiology, 7, 14, (2016-11-09) |
op_relation |
https://doi.org/10.3389/fmicb.2016.01781 https://zenodo.org/communities/sponges https://zenodo.org/communities/eu oai:zenodo.org:196262 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.3389/fmicb.2016.01781 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
_version_ |
1809763767087529984 |