Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis
Background: The periodontal pathogen Porphyromonas gingivalis is hypothesized to be important in rheumatoid arthritis (RA) aetiology by inducing production of anti-citrullinated protein antibodies (ACPA). We have shown that ACPA precede RA onset by years, and that anti- P. gingivalis antibody levels...
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ftzenodo:oai:zenodo.org:160072 2024-09-09T19:59:46+00:00 Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin 2016-09-07 https://doi.org/10.1186/s13075-016-1100-4 unknown Zenodo https://zenodo.org/communities/fp7-bmc https://zenodo.org/communities/eu https://doi.org/10.1186/s13075-016-1100-4 oai:zenodo.org:160072 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Arthritis Research & Therapy, 18(1), 201, (2016-09-07) Porphyromonas gingivalis Antibodies Arginine gingipainB Citrullinated peptide Rheumatoid arthritis Predating onset Anti-citrullinated protein/peptide antibodies info:eu-repo/semantics/article 2016 ftzenodo https://doi.org/10.1186/s13075-016-1100-4 2024-07-25T15:46:44Z Background: The periodontal pathogen Porphyromonas gingivalis is hypothesized to be important in rheumatoid arthritis (RA) aetiology by inducing production of anti-citrullinated protein antibodies (ACPA). We have shown that ACPA precede RA onset by years, and that anti- P. gingivalis antibody levels are elevated in RA patients. The aim of this study was to investigate whether anti- P. gingivalis antibodies pre-date symptom onset and ACPA production. Methods: A case–control study (251 cases, 198 controls) was performed within the Biobank of Northern Sweden. Cases had donated blood samples ( n = 422) before the onset of RA symptoms by 5.2 (6.2) years (median (interquartile range)). Blood was also collected from 192 RA patients following diagnosis. Antibodies against P. gingivalis virulence factor arginine gingipainB (RgpB), and a citrullinated peptide (CPP3) derived from the P. gingivalis peptidylarginine deiminase enzyme, were analysed by ELISA. Results: Anti-RgpB IgG levels were significantly increased in pre-symptomatic individuals (mean ± SEM; 152.7 ± 14.8 AU/ml) and in RA patients (114.4 ± 16.9 AU/ml), compared with controls ( p < 0.001). Anti-CPP3 antibodies were detected in 5 % of pre-symptomatic individuals and in 8 % of RA patients, with elevated levels in both subsets (4.33 ± 0.59 and 9.29 ± 1.81 AU/ml, respectively) compared with controls ( p < 0.001). Anti-CPP3 antibodies followed the ACPA response, with increasing concentrations over time, whilst anti-RgpB antibodies were elevated and stable in the pre-symptomatic individuals with a trend towards lower levels after RA diagnosis. Conclusions: Anti- P. gingivalis antibody concentrations were significantly increased in RA patients compared with controls, and were detectable years before onset of symptoms of RA, supporting an aetiological role for P. gingivalis in the development of RA. Article in Journal/Newspaper Northern Sweden Zenodo Arthritis Research & Therapy 18 1 |
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Porphyromonas gingivalis Antibodies Arginine gingipainB Citrullinated peptide Rheumatoid arthritis Predating onset Anti-citrullinated protein/peptide antibodies |
spellingShingle |
Porphyromonas gingivalis Antibodies Arginine gingipainB Citrullinated peptide Rheumatoid arthritis Predating onset Anti-citrullinated protein/peptide antibodies Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
topic_facet |
Porphyromonas gingivalis Antibodies Arginine gingipainB Citrullinated peptide Rheumatoid arthritis Predating onset Anti-citrullinated protein/peptide antibodies |
description |
Background: The periodontal pathogen Porphyromonas gingivalis is hypothesized to be important in rheumatoid arthritis (RA) aetiology by inducing production of anti-citrullinated protein antibodies (ACPA). We have shown that ACPA precede RA onset by years, and that anti- P. gingivalis antibody levels are elevated in RA patients. The aim of this study was to investigate whether anti- P. gingivalis antibodies pre-date symptom onset and ACPA production. Methods: A case–control study (251 cases, 198 controls) was performed within the Biobank of Northern Sweden. Cases had donated blood samples ( n = 422) before the onset of RA symptoms by 5.2 (6.2) years (median (interquartile range)). Blood was also collected from 192 RA patients following diagnosis. Antibodies against P. gingivalis virulence factor arginine gingipainB (RgpB), and a citrullinated peptide (CPP3) derived from the P. gingivalis peptidylarginine deiminase enzyme, were analysed by ELISA. Results: Anti-RgpB IgG levels were significantly increased in pre-symptomatic individuals (mean ± SEM; 152.7 ± 14.8 AU/ml) and in RA patients (114.4 ± 16.9 AU/ml), compared with controls ( p < 0.001). Anti-CPP3 antibodies were detected in 5 % of pre-symptomatic individuals and in 8 % of RA patients, with elevated levels in both subsets (4.33 ± 0.59 and 9.29 ± 1.81 AU/ml, respectively) compared with controls ( p < 0.001). Anti-CPP3 antibodies followed the ACPA response, with increasing concentrations over time, whilst anti-RgpB antibodies were elevated and stable in the pre-symptomatic individuals with a trend towards lower levels after RA diagnosis. Conclusions: Anti- P. gingivalis antibody concentrations were significantly increased in RA patients compared with controls, and were detectable years before onset of symptoms of RA, supporting an aetiological role for P. gingivalis in the development of RA. |
format |
Article in Journal/Newspaper |
author |
Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin |
author_facet |
Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin |
author_sort |
Johansson, Linda |
title |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_short |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_full |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_fullStr |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_full_unstemmed |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_sort |
concentration of antibodies against porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
publisher |
Zenodo |
publishDate |
2016 |
url |
https://doi.org/10.1186/s13075-016-1100-4 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Arthritis Research & Therapy, 18(1), 201, (2016-09-07) |
op_relation |
https://zenodo.org/communities/fp7-bmc https://zenodo.org/communities/eu https://doi.org/10.1186/s13075-016-1100-4 oai:zenodo.org:160072 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.1186/s13075-016-1100-4 |
container_title |
Arthritis Research & Therapy |
container_volume |
18 |
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1 |
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1809930839217143808 |