Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix
Abstract — The aim of this study was to synthesize isopropyl myristate, an emollient ester, in a continuous-flow packed-bed reactor using Candida antarctica lipase immobilized on poly(styrene-co-divinylbenzene) matrix prepared by suspension polymerization and magnetized by co-precipitation of Fe 2+...
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ftzenodo:oai:zenodo.org:1474108 2024-09-15T17:45:13+00:00 Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix Mateus V. C. Silva Caio A. P. de Souza Pedro C. de Oliveira Heizir F. de Castro Larissa Freitas 2018-10-31 https://doi.org/10.5281/zenodo.1474108 eng eng Zenodo https://doi.org/10.5281/zenodo.1474107 https://doi.org/10.5281/zenodo.1474108 oai:zenodo.org:1474108 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode International Journal of Engineering Research & Science, 4(10), 12-20, (2018-10-31) esterification isopropyl myristate lipase immobilized magnetic particles packed-bed reactor info:eu-repo/semantics/article 2018 ftzenodo https://doi.org/10.5281/zenodo.147410810.5281/zenodo.1474107 2024-07-25T19:26:56Z Abstract — The aim of this study was to synthesize isopropyl myristate, an emollient ester, in a continuous-flow packed-bed reactor using Candida antarctica lipase immobilized on poly(styrene-co-divinylbenzene) matrix prepared by suspension polymerization and magnetized by co-precipitation of Fe 2+ and Fe 3+ in alkaline medium. To determine the best esterification conditions, we investigated the effects of acid/alcohol molar ratio (1:5, 1:10, and 1:15) on reaction yield in shake flasks. The three tested conditions provided similar results, esterification yields of approximately 80%. An acid/alcohol molar ratio of 1:15 was chosen for further experiments because it allowed for better operability of the bioreactor. Subsequently, we compared the reactor performance in up flow and down flow modes. This experiment showed that greater ease of operation was achieved with down flow operation. We also evaluated the influence of space time (8 and 20 h) on reaction yield and productivity. A space time of 8 h provided better results. An experimental system consisting of two bioreactors and a molecular sieve packed column was used to remove the water formed during esterification and thus increase the yield of isopropyl myristate. There was a significant improvement in performance with the use of the two-stage system, which resulted in almost complete conversion of reagents, an increase of about 150% in biocatalyst half-life, and an isopropyl myristate productivity of 25 g L −1 h −1 , confirming the beneficial effect of adding a water extraction column to the experimental system. Article in Journal/Newspaper Antarc* Antarctica Zenodo |
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op_collection_id |
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language |
English |
topic |
esterification isopropyl myristate lipase immobilized magnetic particles packed-bed reactor |
spellingShingle |
esterification isopropyl myristate lipase immobilized magnetic particles packed-bed reactor Mateus V. C. Silva Caio A. P. de Souza Pedro C. de Oliveira Heizir F. de Castro Larissa Freitas Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
topic_facet |
esterification isopropyl myristate lipase immobilized magnetic particles packed-bed reactor |
description |
Abstract — The aim of this study was to synthesize isopropyl myristate, an emollient ester, in a continuous-flow packed-bed reactor using Candida antarctica lipase immobilized on poly(styrene-co-divinylbenzene) matrix prepared by suspension polymerization and magnetized by co-precipitation of Fe 2+ and Fe 3+ in alkaline medium. To determine the best esterification conditions, we investigated the effects of acid/alcohol molar ratio (1:5, 1:10, and 1:15) on reaction yield in shake flasks. The three tested conditions provided similar results, esterification yields of approximately 80%. An acid/alcohol molar ratio of 1:15 was chosen for further experiments because it allowed for better operability of the bioreactor. Subsequently, we compared the reactor performance in up flow and down flow modes. This experiment showed that greater ease of operation was achieved with down flow operation. We also evaluated the influence of space time (8 and 20 h) on reaction yield and productivity. A space time of 8 h provided better results. An experimental system consisting of two bioreactors and a molecular sieve packed column was used to remove the water formed during esterification and thus increase the yield of isopropyl myristate. There was a significant improvement in performance with the use of the two-stage system, which resulted in almost complete conversion of reagents, an increase of about 150% in biocatalyst half-life, and an isopropyl myristate productivity of 25 g L −1 h −1 , confirming the beneficial effect of adding a water extraction column to the experimental system. |
format |
Article in Journal/Newspaper |
author |
Mateus V. C. Silva Caio A. P. de Souza Pedro C. de Oliveira Heizir F. de Castro Larissa Freitas |
author_facet |
Mateus V. C. Silva Caio A. P. de Souza Pedro C. de Oliveira Heizir F. de Castro Larissa Freitas |
author_sort |
Mateus V. C. Silva |
title |
Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
title_short |
Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
title_full |
Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
title_fullStr |
Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
title_full_unstemmed |
Isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
title_sort |
isopropyl myristate continuous synthesis in a packed-bed reactor using lipase immobilized on magnetic polymer matrix |
publisher |
Zenodo |
publishDate |
2018 |
url |
https://doi.org/10.5281/zenodo.1474108 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
International Journal of Engineering Research & Science, 4(10), 12-20, (2018-10-31) |
op_relation |
https://doi.org/10.5281/zenodo.1474107 https://doi.org/10.5281/zenodo.1474108 oai:zenodo.org:1474108 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.147410810.5281/zenodo.1474107 |
_version_ |
1810492950254190592 |