Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis —the paradigm of mesophilic hydrocarbonoclastic bacteria— O. a...
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ftzenodo:oai:zenodo.org:13911 2024-09-15T17:42:52+00:00 Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica Kube, Michael Tatyana N. Chernikova Yamal Al-Ramahi Ana Beloqui Nieves Lopez-Cortez Maria-Eugenia Guazzaroni Hermann J. Heipieper Sven Klages Oleg R. Kotsyurbenko Ines Langer Taras Y. Nechitaylo Heinrich Lünsdorf Marisol Fernandez Silvia Juarez Sergio Ciordia Alexander Singer Olga Kagan Olga Egorova Pierre Alain Peti Peter Stogios Youngchang Kim Anatoli Tchigvintsev Robert Flick Renata Denaro Maria Genovese Juan P. Alba Oleg N. Reva Montserrat Martınez-Gomariz Hai Tran Manuel Ferrer Alexei Savchenko Alexander F. Yakunin Michail M. Yakimov Olga V. Golyshina Richard Reinhardt Peter N. Golyshin 2013-07-23 https://doi.org/10.1038/ncomms3156 unknown Zenodo https://zenodo.org/communities/eu https://doi.org/10.1038/ncomms3156 oai:zenodo.org:13911 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Nature Communications, 4(2013), 2156, (2013-07-23) info:eu-repo/semantics/article 2013 ftzenodo https://doi.org/10.1038/ncomms3156 2024-07-25T12:34:16Z Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis —the paradigm of mesophilic hydrocarbonoclastic bacteria— O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. Article in Journal/Newspaper Antarc* Antarctica Zenodo Nature Communications 4 1 |
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Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis —the paradigm of mesophilic hydrocarbonoclastic bacteria— O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. |
format |
Article in Journal/Newspaper |
author |
Kube, Michael Tatyana N. Chernikova Yamal Al-Ramahi Ana Beloqui Nieves Lopez-Cortez Maria-Eugenia Guazzaroni Hermann J. Heipieper Sven Klages Oleg R. Kotsyurbenko Ines Langer Taras Y. Nechitaylo Heinrich Lünsdorf Marisol Fernandez Silvia Juarez Sergio Ciordia Alexander Singer Olga Kagan Olga Egorova Pierre Alain Peti Peter Stogios Youngchang Kim Anatoli Tchigvintsev Robert Flick Renata Denaro Maria Genovese Juan P. Alba Oleg N. Reva Montserrat Martınez-Gomariz Hai Tran Manuel Ferrer Alexei Savchenko Alexander F. Yakunin Michail M. Yakimov Olga V. Golyshina Richard Reinhardt Peter N. Golyshin |
spellingShingle |
Kube, Michael Tatyana N. Chernikova Yamal Al-Ramahi Ana Beloqui Nieves Lopez-Cortez Maria-Eugenia Guazzaroni Hermann J. Heipieper Sven Klages Oleg R. Kotsyurbenko Ines Langer Taras Y. Nechitaylo Heinrich Lünsdorf Marisol Fernandez Silvia Juarez Sergio Ciordia Alexander Singer Olga Kagan Olga Egorova Pierre Alain Peti Peter Stogios Youngchang Kim Anatoli Tchigvintsev Robert Flick Renata Denaro Maria Genovese Juan P. Alba Oleg N. Reva Montserrat Martınez-Gomariz Hai Tran Manuel Ferrer Alexei Savchenko Alexander F. Yakunin Michail M. Yakimov Olga V. Golyshina Richard Reinhardt Peter N. Golyshin Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
author_facet |
Kube, Michael Tatyana N. Chernikova Yamal Al-Ramahi Ana Beloqui Nieves Lopez-Cortez Maria-Eugenia Guazzaroni Hermann J. Heipieper Sven Klages Oleg R. Kotsyurbenko Ines Langer Taras Y. Nechitaylo Heinrich Lünsdorf Marisol Fernandez Silvia Juarez Sergio Ciordia Alexander Singer Olga Kagan Olga Egorova Pierre Alain Peti Peter Stogios Youngchang Kim Anatoli Tchigvintsev Robert Flick Renata Denaro Maria Genovese Juan P. Alba Oleg N. Reva Montserrat Martınez-Gomariz Hai Tran Manuel Ferrer Alexei Savchenko Alexander F. Yakunin Michail M. Yakimov Olga V. Golyshina Richard Reinhardt Peter N. Golyshin |
author_sort |
Kube, Michael |
title |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_short |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_full |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_fullStr |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_full_unstemmed |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_sort |
genome sequence and functional genomic analysis of the oil-degrading bacterium oleispira antarctica |
publisher |
Zenodo |
publishDate |
2013 |
url |
https://doi.org/10.1038/ncomms3156 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Nature Communications, 4(2013), 2156, (2013-07-23) |
op_relation |
https://zenodo.org/communities/eu https://doi.org/10.1038/ncomms3156 oai:zenodo.org:13911 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.1038/ncomms3156 |
container_title |
Nature Communications |
container_volume |
4 |
container_issue |
1 |
_version_ |
1810489651333431296 |