Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum
The gray wolf (Canis lupus) was found to be a new natural definitive host for Neospora caninum. Neospora-like oocysts were found microscopically in the feces of three of 73 wolves from Minnesota examined at necropsy. N. caninum-specific DNA was amplified from the oocysts of all three wolves. Oocysts...
Published in: | Veterinary Parasitology |
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Online Access: | https://doi.org/10.1016/j.vetpar.2011.05.018 |
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ftzenodo:oai:zenodo.org:1259437 2024-09-09T19:35:20+00:00 Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum Dubey, J. P. Jenkins, M. C. Rajendran, C. Miska, K. Ferreira, L. R. Martins, J. Kwok, O. C. H. Choudhary, S. 2011-05-20 https://doi.org/10.1016/j.vetpar.2011.05.018 unknown Zenodo https://doi.org/10.1016/j.vetpar.2011.05.018 oai:zenodo.org:1259437 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode info:eu-repo/semantics/article 2011 ftzenodo https://doi.org/10.1016/j.vetpar.2011.05.018 2024-07-27T05:46:14Z The gray wolf (Canis lupus) was found to be a new natural definitive host for Neospora caninum. Neospora-like oocysts were found microscopically in the feces of three of 73 wolves from Minnesota examined at necropsy. N. caninum-specific DNA was amplified from the oocysts of all three wolves. Oocysts from one wolf were infective for the gamma interferon gene knock out (KO) mice. Viable N. caninum (designated NcWolfUS1) was isolated in cell cultures seeded with tissue homogenate from the infected mouse. Typical thick walled tissue cysts were found in outbred mice inoculated with the parasite from the KO mouse. Tissue stages in mice stained positively with N. caninum-specific polyclonal antibodies. Our observation suggests that wolves may be an important link in the sylvatic cycle of N. caninum. Article in Journal/Newspaper Canis lupus gray wolf Zenodo Veterinary Parasitology 181 2-4 382 387 |
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Open Polar |
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ftzenodo |
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unknown |
description |
The gray wolf (Canis lupus) was found to be a new natural definitive host for Neospora caninum. Neospora-like oocysts were found microscopically in the feces of three of 73 wolves from Minnesota examined at necropsy. N. caninum-specific DNA was amplified from the oocysts of all three wolves. Oocysts from one wolf were infective for the gamma interferon gene knock out (KO) mice. Viable N. caninum (designated NcWolfUS1) was isolated in cell cultures seeded with tissue homogenate from the infected mouse. Typical thick walled tissue cysts were found in outbred mice inoculated with the parasite from the KO mouse. Tissue stages in mice stained positively with N. caninum-specific polyclonal antibodies. Our observation suggests that wolves may be an important link in the sylvatic cycle of N. caninum. |
format |
Article in Journal/Newspaper |
author |
Dubey, J. P. Jenkins, M. C. Rajendran, C. Miska, K. Ferreira, L. R. Martins, J. Kwok, O. C. H. Choudhary, S. |
spellingShingle |
Dubey, J. P. Jenkins, M. C. Rajendran, C. Miska, K. Ferreira, L. R. Martins, J. Kwok, O. C. H. Choudhary, S. Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum |
author_facet |
Dubey, J. P. Jenkins, M. C. Rajendran, C. Miska, K. Ferreira, L. R. Martins, J. Kwok, O. C. H. Choudhary, S. |
author_sort |
Dubey, J. P. |
title |
Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum |
title_short |
Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum |
title_full |
Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum |
title_fullStr |
Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum |
title_full_unstemmed |
Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum |
title_sort |
gray wolf (canis lupus) is a natural definitive host for neospora caninum |
publisher |
Zenodo |
publishDate |
2011 |
url |
https://doi.org/10.1016/j.vetpar.2011.05.018 |
genre |
Canis lupus gray wolf |
genre_facet |
Canis lupus gray wolf |
op_relation |
https://doi.org/10.1016/j.vetpar.2011.05.018 oai:zenodo.org:1259437 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.1016/j.vetpar.2011.05.018 |
container_title |
Veterinary Parasitology |
container_volume |
181 |
container_issue |
2-4 |
container_start_page |
382 |
op_container_end_page |
387 |
_version_ |
1809904711581564928 |