A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies
Amphipods constitute an abundant part of Icelandic deep-sea zoobenthos yet knowledge of the diversity of this fauna, particularly at the molecular level, is scarce. The present work aims to use molecular methods to investigate genetic variation of the Amphipoda sampled during two IceAGE collecting e...
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2018
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Online Access: | https://doi.org/10.3897/zookeys.731.19931 |
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ftzenodo:oai:zenodo.org:1166295 2024-09-15T18:23:48+00:00 A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies Jażdżewska, Anna M. Corbari, Laure Driskell, Amy Frutos, Inmaculada Havermans, Charlotte Hendrycks, Ed Hughes, Lauren Lörz, Anne-Nina Stransky, Bente Tandberg, Anne Helene S. Vader, Wim Brix, Saskia 2018-01-23 https://doi.org/10.3897/zookeys.731.19931 unknown Pensoft Publishers https://zenodo.org/communities/biosyslit https://doi.org/10.3897/zookeys.731.19931 oai:zenodo.org:1166295 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode ZooKeys, 731, 55-73, (2018-01-23) Animalia Arthropoda Malacostraca Amphipoda COI barcoding deep sea North Atlantic info:eu-repo/semantics/article 2018 ftzenodo https://doi.org/10.3897/zookeys.731.19931 2024-07-26T11:21:09Z Amphipods constitute an abundant part of Icelandic deep-sea zoobenthos yet knowledge of the diversity of this fauna, particularly at the molecular level, is scarce. The present work aims to use molecular methods to investigate genetic variation of the Amphipoda sampled during two IceAGE collecting expeditions. The mitochondrial cytochrome oxidase subunit 1 (COI) of 167 individuals originally assigned to 75 morphospecies was analysed. These targeted morhospecies were readily identifiable by experts using light microscopy and representative of families where there is current ongoing taxonomic research. The study resulted in 81 Barcode Identity Numbers (BINs) (of which >90% were published for the first time), while Automatic Barcode Gap Discovery revealed the existence of 78 to 83 Molecular Operational Taxonomic Units (MOTUs). Six nominal species (Rhachotropis helleri, Arrhis phyllonyx, Deflexilodes tenuirostratus, Paroediceros propinquus, Metopa boeckii, Astyra abyssi) appeared to have a molecular variation higher than the 0.03 threshold of both p-distance and K2P usually used for amphipod species delineation. Conversely, two Oedicerotidae regarded as separate morphospecies clustered together with divergences in the order of intraspecific variation. The incongruence between the BINs associated with presently identified species and the publicly available data of the same taxa was observed in case of Paramphithoe hystrix and Amphilochus manudens. The findings from this research project highlight the necessity of supporting molecular studies with thorough morphology species analyses. Article in Journal/Newspaper North Atlantic Zenodo ZooKeys 731 55 73 |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
Animalia Arthropoda Malacostraca Amphipoda COI barcoding deep sea North Atlantic |
spellingShingle |
Animalia Arthropoda Malacostraca Amphipoda COI barcoding deep sea North Atlantic Jażdżewska, Anna M. Corbari, Laure Driskell, Amy Frutos, Inmaculada Havermans, Charlotte Hendrycks, Ed Hughes, Lauren Lörz, Anne-Nina Stransky, Bente Tandberg, Anne Helene S. Vader, Wim Brix, Saskia A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies |
topic_facet |
Animalia Arthropoda Malacostraca Amphipoda COI barcoding deep sea North Atlantic |
description |
Amphipods constitute an abundant part of Icelandic deep-sea zoobenthos yet knowledge of the diversity of this fauna, particularly at the molecular level, is scarce. The present work aims to use molecular methods to investigate genetic variation of the Amphipoda sampled during two IceAGE collecting expeditions. The mitochondrial cytochrome oxidase subunit 1 (COI) of 167 individuals originally assigned to 75 morphospecies was analysed. These targeted morhospecies were readily identifiable by experts using light microscopy and representative of families where there is current ongoing taxonomic research. The study resulted in 81 Barcode Identity Numbers (BINs) (of which >90% were published for the first time), while Automatic Barcode Gap Discovery revealed the existence of 78 to 83 Molecular Operational Taxonomic Units (MOTUs). Six nominal species (Rhachotropis helleri, Arrhis phyllonyx, Deflexilodes tenuirostratus, Paroediceros propinquus, Metopa boeckii, Astyra abyssi) appeared to have a molecular variation higher than the 0.03 threshold of both p-distance and K2P usually used for amphipod species delineation. Conversely, two Oedicerotidae regarded as separate morphospecies clustered together with divergences in the order of intraspecific variation. The incongruence between the BINs associated with presently identified species and the publicly available data of the same taxa was observed in case of Paramphithoe hystrix and Amphilochus manudens. The findings from this research project highlight the necessity of supporting molecular studies with thorough morphology species analyses. |
format |
Article in Journal/Newspaper |
author |
Jażdżewska, Anna M. Corbari, Laure Driskell, Amy Frutos, Inmaculada Havermans, Charlotte Hendrycks, Ed Hughes, Lauren Lörz, Anne-Nina Stransky, Bente Tandberg, Anne Helene S. Vader, Wim Brix, Saskia |
author_facet |
Jażdżewska, Anna M. Corbari, Laure Driskell, Amy Frutos, Inmaculada Havermans, Charlotte Hendrycks, Ed Hughes, Lauren Lörz, Anne-Nina Stransky, Bente Tandberg, Anne Helene S. Vader, Wim Brix, Saskia |
author_sort |
Jażdżewska, Anna M. |
title |
A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies |
title_short |
A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies |
title_full |
A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies |
title_fullStr |
A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies |
title_full_unstemmed |
A genetic fingerprint of Amphipoda from Icelandic waters – the baseline for further biodiversity and biogeography studies |
title_sort |
genetic fingerprint of amphipoda from icelandic waters – the baseline for further biodiversity and biogeography studies |
publisher |
Pensoft Publishers |
publishDate |
2018 |
url |
https://doi.org/10.3897/zookeys.731.19931 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ZooKeys, 731, 55-73, (2018-01-23) |
op_relation |
https://zenodo.org/communities/biosyslit https://doi.org/10.3897/zookeys.731.19931 oai:zenodo.org:1166295 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.3897/zookeys.731.19931 |
container_title |
ZooKeys |
container_volume |
731 |
container_start_page |
55 |
op_container_end_page |
73 |
_version_ |
1810464065321959424 |