Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland
Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area...
Main Authors: | , , , , , , , , |
---|---|
Format: | Other/Unknown Material |
Language: | unknown |
Published: |
Zenodo
2024
|
Subjects: | |
Online Access: | https://doi.org/10.5061/dryad.t76hdr87v |
id |
ftzenodo:oai:zenodo.org:11158967 |
---|---|
record_format |
openpolar |
spelling |
ftzenodo:oai:zenodo.org:11158967 2024-09-15T18:16:12+00:00 Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund 2024-05-09 https://doi.org/10.5061/dryad.t76hdr87v unknown Zenodo https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.t76hdr87v oai:zenodo.org:11158967 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode single-nucleotide polymorphism SNP brown bear Ursus arctos Finland Wildlife monitoring DNA info:eu-repo/semantics/other 2024 ftzenodo https://doi.org/10.5061/dryad.t76hdr87v 2024-07-26T17:06:22Z Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area before being deployed. Around 4,500 brown bears ( Ursus arctos ) live in Norway, Sweden, and Finland and they are divided into the western (Scandinavian) and eastern (Karelian) population. Both populations have recovered and are connected via asymmetric migration. DNA-based population monitoring in Norway and Sweden uses the same set of genetic markers. With Finland aiming to implement monitoring, we tested the available SNP-panel developed to assess brown bears in Norway and Sweden, on tissue samples from a representative set of 93 legally harvested individuals from Finland. The aim was to test for ascertainment bias and evaluate its suitability for DNA-based transnational-monitoring covering all three countries. We compared results to the performance of microsatellite genotypes of the same individuals in Finland and against SNP-genotypes from individuals sampled in Sweden ( N =95) and Norway ( N =27). In Finland, a higher resolution for individual identification was obtained for SNPs (PI=1.18E-27) compared to microsatellites (PI=4.2E-11). Compared to Norway and Sweden, probability of identity of the SNP-panel was slightly higher and expected heterozygosity lower in Finland indicating ascertainment bias. Yet, our evaluation show that the available SNP-panel outperforms the microsatellite panel currently applied in Norway and Sweden. The SNP-panel represents a powerful tool that could aid improving transnational DNA-based monitoring of brown bears across these three countries. Funding provided by: Norwegian Institute for Nature Research Crossref Funder Registry ID: https://ror.org/04aha0598 Award Number: 93 tissue samples collected 2011-2017 from legally harvested male brown bears in Finland were genotyped with ... Other/Unknown Material karelia* karelian Ursus arctos Zenodo |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
single-nucleotide polymorphism SNP brown bear Ursus arctos Finland Wildlife monitoring DNA |
spellingShingle |
single-nucleotide polymorphism SNP brown bear Ursus arctos Finland Wildlife monitoring DNA Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland |
topic_facet |
single-nucleotide polymorphism SNP brown bear Ursus arctos Finland Wildlife monitoring DNA |
description |
Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area before being deployed. Around 4,500 brown bears ( Ursus arctos ) live in Norway, Sweden, and Finland and they are divided into the western (Scandinavian) and eastern (Karelian) population. Both populations have recovered and are connected via asymmetric migration. DNA-based population monitoring in Norway and Sweden uses the same set of genetic markers. With Finland aiming to implement monitoring, we tested the available SNP-panel developed to assess brown bears in Norway and Sweden, on tissue samples from a representative set of 93 legally harvested individuals from Finland. The aim was to test for ascertainment bias and evaluate its suitability for DNA-based transnational-monitoring covering all three countries. We compared results to the performance of microsatellite genotypes of the same individuals in Finland and against SNP-genotypes from individuals sampled in Sweden ( N =95) and Norway ( N =27). In Finland, a higher resolution for individual identification was obtained for SNPs (PI=1.18E-27) compared to microsatellites (PI=4.2E-11). Compared to Norway and Sweden, probability of identity of the SNP-panel was slightly higher and expected heterozygosity lower in Finland indicating ascertainment bias. Yet, our evaluation show that the available SNP-panel outperforms the microsatellite panel currently applied in Norway and Sweden. The SNP-panel represents a powerful tool that could aid improving transnational DNA-based monitoring of brown bears across these three countries. Funding provided by: Norwegian Institute for Nature Research Crossref Funder Registry ID: https://ror.org/04aha0598 Award Number: 93 tissue samples collected 2011-2017 from legally harvested male brown bears in Finland were genotyped with ... |
format |
Other/Unknown Material |
author |
Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund |
author_facet |
Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund |
author_sort |
Kopatz, Alexander |
title |
Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland |
title_short |
Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland |
title_full |
Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland |
title_fullStr |
Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland |
title_full_unstemmed |
Autosomal SNP-genotype data of brown bears (Ursus arctos) in Finland |
title_sort |
autosomal snp-genotype data of brown bears (ursus arctos) in finland |
publisher |
Zenodo |
publishDate |
2024 |
url |
https://doi.org/10.5061/dryad.t76hdr87v |
genre |
karelia* karelian Ursus arctos |
genre_facet |
karelia* karelian Ursus arctos |
op_relation |
https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.t76hdr87v oai:zenodo.org:11158967 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.t76hdr87v |
_version_ |
1810454210939977728 |