Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences
We sequenced amplicons targeting a segment of the 12S ribosomal DNA gene in vertebrates from DNA pools isolated from sections of a sediment core from Chisholm Lake in interior Alaska. The primary aim of this sequencing effort was to add to our understanding of the evolution of fish faunas in Beringi...
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ftzenodo:oai:zenodo.org:10983375 2024-09-15T18:41:29+00:00 Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences Lopez, Andres 2024-04-17 https://doi.org/10.5061/dryad.08kprr58x unknown Zenodo https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.08kprr58x oai:zenodo.org:10983375 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode metabarcoding MiFish sedimentary DNA Beringia info:eu-repo/semantics/other 2024 ftzenodo https://doi.org/10.5061/dryad.08kprr58x 2024-07-26T01:30:35Z We sequenced amplicons targeting a segment of the 12S ribosomal DNA gene in vertebrates from DNA pools isolated from sections of a sediment core from Chisholm Lake in interior Alaska. The primary aim of this sequencing effort was to add to our understanding of the evolution of fish faunas in Beringian regions impacted by glacial cycles. This dataset consists of unprocessed output from amplicon paired end short read sequencing on an Illumina sequencing by synthesis platform. Analyses of these sequences are being incorporated into a broader effort to examine post-glacial Beringian biotas and ecologies. Funding provided by: National Science Foundation Crossref Funder Registry ID: https://ror.org/021nxhr62 Award Number: 1801222 DNA was isolated from sections from a lake bottom sediment core. DNA isolates were used as PCR templates using the reagents described in the MiFish metabarcoding protocols of Miya et al. (2015). Amplicons were then used to prepare libraries suitable for sequencing on Illumina platforms. All libraries generated from core sections and section replicates were uniquely indexed then pooled together and sequenced on a MiSeq instrument. Other/Unknown Material Alaska Beringia Zenodo |
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metabarcoding MiFish sedimentary DNA Beringia |
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metabarcoding MiFish sedimentary DNA Beringia Lopez, Andres Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences |
topic_facet |
metabarcoding MiFish sedimentary DNA Beringia |
description |
We sequenced amplicons targeting a segment of the 12S ribosomal DNA gene in vertebrates from DNA pools isolated from sections of a sediment core from Chisholm Lake in interior Alaska. The primary aim of this sequencing effort was to add to our understanding of the evolution of fish faunas in Beringian regions impacted by glacial cycles. This dataset consists of unprocessed output from amplicon paired end short read sequencing on an Illumina sequencing by synthesis platform. Analyses of these sequences are being incorporated into a broader effort to examine post-glacial Beringian biotas and ecologies. Funding provided by: National Science Foundation Crossref Funder Registry ID: https://ror.org/021nxhr62 Award Number: 1801222 DNA was isolated from sections from a lake bottom sediment core. DNA isolates were used as PCR templates using the reagents described in the MiFish metabarcoding protocols of Miya et al. (2015). Amplicons were then used to prepare libraries suitable for sequencing on Illumina platforms. All libraries generated from core sections and section replicates were uniquely indexed then pooled together and sequenced on a MiSeq instrument. |
format |
Other/Unknown Material |
author |
Lopez, Andres |
author_facet |
Lopez, Andres |
author_sort |
Lopez, Andres |
title |
Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences |
title_short |
Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences |
title_full |
Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences |
title_fullStr |
Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences |
title_full_unstemmed |
Chisholm Lake sedimentary unprocessed fish metabarcoding DNA sequences |
title_sort |
chisholm lake sedimentary unprocessed fish metabarcoding dna sequences |
publisher |
Zenodo |
publishDate |
2024 |
url |
https://doi.org/10.5061/dryad.08kprr58x |
genre |
Alaska Beringia |
genre_facet |
Alaska Beringia |
op_relation |
https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.08kprr58x oai:zenodo.org:10983375 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.08kprr58x |
_version_ |
1810485886230462464 |