Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture.
Data from Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. Files in .JBN and .xlsx format Fish cell culture is a common in vitro tool for studies in different fields such as virology, toxicology, pat...
Main Authors: | , , , , |
---|---|
Format: | Other/Unknown Material |
Language: | English |
Published: |
Zenodo
2023
|
Subjects: | |
Online Access: | https://doi.org/10.5281/zenodo.10436722 |
id |
ftzenodo:oai:zenodo.org:10436722 |
---|---|
record_format |
openpolar |
spelling |
ftzenodo:oai:zenodo.org:10436722 2024-09-09T20:05:55+00:00 Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. Nualart, Daniela P. Dann, Francisco Oyarzún-Salazar, Ricardo Morera, Francisco J. Vargas-Chacoff, Luis 2023-12-27 https://doi.org/10.5281/zenodo.10436722 eng eng Zenodo https://doi.org/10.3390/biology12070924 https://doi.org/10.5281/zenodo.10436721 https://doi.org/10.5281/zenodo.10436722 oai:zenodo.org:10436722 info:eu-repo/semantics/restrictedAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode immune response head kidney cells primary culture LPS POLY I:C salmonids info:eu-repo/semantics/other 2023 ftzenodo https://doi.org/10.5281/zenodo.1043672210.3390/biology1207092410.5281/zenodo.10436721 2024-07-27T01:24:48Z Data from Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. Files in .JBN and .xlsx format Fish cell culture is a common in vitro tool for studies in different fields such as virology, toxicology, pathology and immunology of fish. Fish cell cultures are a promising help to study how to diagnose and control relevant viral and intracellular bacterial infections in aquaculture. They can also be used for developing vaccines and immunostimulants, especially with the ethical demand aiming to reduce and replace the number of fish used in research. This study aimed to isolate head kidney primary cell cultures from three Chilean salmonids: Salmo salar , Oncorhynchus kisutch , and Oncorhynchus mykiss , and characterize the response to bacterial and viral stimuli by evaluating various markers of the innate and adaptive immune response. Specifically, the primary cell cultures of the head kidney from the three salmonids studied were cultured and exposed to two substances that mimic molecular patterns of different pathogens, i.e., Lipopolysaccharide (LPS) (bacterial) and Polyinosinic: polycytidylic acid (POLY I:C). Subsequently, we determined the mRNA expression profiles of the TLR-1, TLR-8, IgM, TLR-5, and MHC II genes. Head kidney primary cell cultures from the three species grown in vitro responded differently to POLY I:C and LPS. This is the first study to demonstrate and characterize the expression of immune genes in head kidney primary cell culture isolated from three salmonid species. It also indicates their potential role in developing immune responses as defense response agents and targets of immunoregulatory factors. This work was supported by Fondecyt Regular (1160877) and (1180957), Fondap-Ideal 15150003, ANID-Millennium Science Initiative Program-Center code “ICN2021_002”. D. Nualart awarded the scholarship ANID-Millennium Science Initiative Program-Center code “ICN2021_002”. Other/Unknown Material Salmo salar Zenodo |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
English |
topic |
immune response head kidney cells primary culture LPS POLY I:C salmonids |
spellingShingle |
immune response head kidney cells primary culture LPS POLY I:C salmonids Nualart, Daniela P. Dann, Francisco Oyarzún-Salazar, Ricardo Morera, Francisco J. Vargas-Chacoff, Luis Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. |
topic_facet |
immune response head kidney cells primary culture LPS POLY I:C salmonids |
description |
Data from Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. Files in .JBN and .xlsx format Fish cell culture is a common in vitro tool for studies in different fields such as virology, toxicology, pathology and immunology of fish. Fish cell cultures are a promising help to study how to diagnose and control relevant viral and intracellular bacterial infections in aquaculture. They can also be used for developing vaccines and immunostimulants, especially with the ethical demand aiming to reduce and replace the number of fish used in research. This study aimed to isolate head kidney primary cell cultures from three Chilean salmonids: Salmo salar , Oncorhynchus kisutch , and Oncorhynchus mykiss , and characterize the response to bacterial and viral stimuli by evaluating various markers of the innate and adaptive immune response. Specifically, the primary cell cultures of the head kidney from the three salmonids studied were cultured and exposed to two substances that mimic molecular patterns of different pathogens, i.e., Lipopolysaccharide (LPS) (bacterial) and Polyinosinic: polycytidylic acid (POLY I:C). Subsequently, we determined the mRNA expression profiles of the TLR-1, TLR-8, IgM, TLR-5, and MHC II genes. Head kidney primary cell cultures from the three species grown in vitro responded differently to POLY I:C and LPS. This is the first study to demonstrate and characterize the expression of immune genes in head kidney primary cell culture isolated from three salmonid species. It also indicates their potential role in developing immune responses as defense response agents and targets of immunoregulatory factors. This work was supported by Fondecyt Regular (1160877) and (1180957), Fondap-Ideal 15150003, ANID-Millennium Science Initiative Program-Center code “ICN2021_002”. D. Nualart awarded the scholarship ANID-Millennium Science Initiative Program-Center code “ICN2021_002”. |
format |
Other/Unknown Material |
author |
Nualart, Daniela P. Dann, Francisco Oyarzún-Salazar, Ricardo Morera, Francisco J. Vargas-Chacoff, Luis |
author_facet |
Nualart, Daniela P. Dann, Francisco Oyarzún-Salazar, Ricardo Morera, Francisco J. Vargas-Chacoff, Luis |
author_sort |
Nualart, Daniela P. |
title |
Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. |
title_short |
Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. |
title_full |
Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. |
title_fullStr |
Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. |
title_full_unstemmed |
Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. |
title_sort |
data from: immune transcriptional response in head kidney primary cell cultures isolated from the three most important species in chilean salmonids aquaculture. |
publisher |
Zenodo |
publishDate |
2023 |
url |
https://doi.org/10.5281/zenodo.10436722 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
https://doi.org/10.3390/biology12070924 https://doi.org/10.5281/zenodo.10436721 https://doi.org/10.5281/zenodo.10436722 oai:zenodo.org:10436722 |
op_rights |
info:eu-repo/semantics/restrictedAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.1043672210.3390/biology1207092410.5281/zenodo.10436721 |
_version_ |
1809938333632036864 |