Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding
Background: Biodiversity surveys have long depended on traditional methods of taxonomy to inform sampling protocols and to determine when a representative sample of a given species pool of interest has been obtained. Questions remain as to how to design appropriate sampling efforts to accurately est...
Main Authors: | , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC Ecology
2013
|
Subjects: | |
Online Access: | http://hdl.handle.net/10315/26694 |
id |
ftyorkuniv:oai:yorkspace.library.yorku.ca:10315/26694 |
---|---|
record_format |
openpolar |
spelling |
ftyorkuniv:oai:yorkspace.library.yorku.ca:10315/26694 2023-05-15T15:55:05+02:00 Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding Laforest, Brandon Winegardner, Amanda Zaheer, Omar Jeffery, Nicholas Boyle, Elizabeth Adamowicz, Sarah 2013 application/pdf http://hdl.handle.net/10315/26694 en eng BMC Ecology BMC Ecology 13.13 (2013): 1-17. http://hdl.handle.net/10315/26694 http://www.biomedcentral.com/1472-6785/13/13 http://www.biomedcentral.com/bmcecol/ Ostracoda Crustacea Barcoding biotas Sampling strategy Bioblitz Citizen science Species richness Zooplankton Accumulation curves Subarctic Churchill Manitoba DNA barcoding Article 2013 ftyorkuniv 2022-08-22T13:04:21Z Background: Biodiversity surveys have long depended on traditional methods of taxonomy to inform sampling protocols and to determine when a representative sample of a given species pool of interest has been obtained. Questions remain as to how to design appropriate sampling efforts to accurately estimate total biodiversity. Here we consider the biodiversity of freshwater ostracods (crustacean class Ostracoda) from the region of Churchill, Manitoba, Canada. Through an analysis of observed species richness and complementarity, accumulation curves, and richness estimators, we conduct an a posteriori analysis of five bioblitz-style collection strategies that differed in terms of total duration, number of sites, protocol flexibility to heterogeneous habitats, sorting of specimens for analysis, and primary purpose of collection. We used DNA barcoding to group specimens into molecular operational taxonomic units for comparison. Results: Forty-eight provisional species were identified through genetic divergences, up from the 30 species previously known and documented in literature from the Churchill region. We found differential sampling efficiency among the five strategies, with liberal sorting of specimens for molecular analysis, protocol flexibility (and particularly a focus on covering diverse microhabitats), and a taxon-specific focus to collection having strong influences on garnering more accurate species richness estimates. Conclusions: Our findings have implications for the successful design of future biodiversity surveys and citizen-science collection projects, which are becoming increasingly popular and have been shown to produce reliable results for a variety of taxa despite relying on largely untrained collectors. We propose that efficiency of biodiversity surveys can be increased by non-experts deliberately selecting diverse microhabitats; by conducting two rounds of molecular analysis, with the numbers of samples processed during round two informed by the singleton prevalence during round one; and by ... Article in Journal/Newspaper Churchill Subarctic York University, Toronto: YorkSpace Canada |
institution |
Open Polar |
collection |
York University, Toronto: YorkSpace |
op_collection_id |
ftyorkuniv |
language |
English |
topic |
Ostracoda Crustacea Barcoding biotas Sampling strategy Bioblitz Citizen science Species richness Zooplankton Accumulation curves Subarctic Churchill Manitoba DNA barcoding |
spellingShingle |
Ostracoda Crustacea Barcoding biotas Sampling strategy Bioblitz Citizen science Species richness Zooplankton Accumulation curves Subarctic Churchill Manitoba DNA barcoding Laforest, Brandon Winegardner, Amanda Zaheer, Omar Jeffery, Nicholas Boyle, Elizabeth Adamowicz, Sarah Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding |
topic_facet |
Ostracoda Crustacea Barcoding biotas Sampling strategy Bioblitz Citizen science Species richness Zooplankton Accumulation curves Subarctic Churchill Manitoba DNA barcoding |
description |
Background: Biodiversity surveys have long depended on traditional methods of taxonomy to inform sampling protocols and to determine when a representative sample of a given species pool of interest has been obtained. Questions remain as to how to design appropriate sampling efforts to accurately estimate total biodiversity. Here we consider the biodiversity of freshwater ostracods (crustacean class Ostracoda) from the region of Churchill, Manitoba, Canada. Through an analysis of observed species richness and complementarity, accumulation curves, and richness estimators, we conduct an a posteriori analysis of five bioblitz-style collection strategies that differed in terms of total duration, number of sites, protocol flexibility to heterogeneous habitats, sorting of specimens for analysis, and primary purpose of collection. We used DNA barcoding to group specimens into molecular operational taxonomic units for comparison. Results: Forty-eight provisional species were identified through genetic divergences, up from the 30 species previously known and documented in literature from the Churchill region. We found differential sampling efficiency among the five strategies, with liberal sorting of specimens for molecular analysis, protocol flexibility (and particularly a focus on covering diverse microhabitats), and a taxon-specific focus to collection having strong influences on garnering more accurate species richness estimates. Conclusions: Our findings have implications for the successful design of future biodiversity surveys and citizen-science collection projects, which are becoming increasingly popular and have been shown to produce reliable results for a variety of taxa despite relying on largely untrained collectors. We propose that efficiency of biodiversity surveys can be increased by non-experts deliberately selecting diverse microhabitats; by conducting two rounds of molecular analysis, with the numbers of samples processed during round two informed by the singleton prevalence during round one; and by ... |
format |
Article in Journal/Newspaper |
author |
Laforest, Brandon Winegardner, Amanda Zaheer, Omar Jeffery, Nicholas Boyle, Elizabeth Adamowicz, Sarah |
author_facet |
Laforest, Brandon Winegardner, Amanda Zaheer, Omar Jeffery, Nicholas Boyle, Elizabeth Adamowicz, Sarah |
author_sort |
Laforest, Brandon |
title |
Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding |
title_short |
Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding |
title_full |
Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding |
title_fullStr |
Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding |
title_full_unstemmed |
Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding |
title_sort |
insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and dna barcoding |
publisher |
BMC Ecology |
publishDate |
2013 |
url |
http://hdl.handle.net/10315/26694 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Churchill Subarctic |
genre_facet |
Churchill Subarctic |
op_relation |
BMC Ecology 13.13 (2013): 1-17. http://hdl.handle.net/10315/26694 |
op_rights |
http://www.biomedcentral.com/1472-6785/13/13 http://www.biomedcentral.com/bmcecol/ |
_version_ |
1766390419508166656 |