Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines
Studies on pharmaceutical and medicine applications of cold adapted polar microalgae are very limited. In this study, it is aimed to evaluate the cytotoxic activity of polar microalgae extracts against different cancer cell lines. In this context, the cultivation of Chlorella variabilis YTU.ANTARCTI...
Published in: | Biochemical Engineering Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2023
|
Subjects: | |
Online Access: | https://avesis.yildiz.edu.tr/publication/details/fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c/oai https://doi.org/10.1016/j.bej.2023.108900 |
id |
ftyildiztuniv:fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c |
---|---|
record_format |
openpolar |
spelling |
ftyildiztuniv:fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c 2023-09-26T15:10:11+02:00 Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines Koç, Rabia Çakır Mutlu, Betül İNAN, Benan ÖZÇİMEN, Didem KARACA, Gülcan Ayşin 2023-06-01T00:00:00Z https://avesis.yildiz.edu.tr/publication/details/fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c/oai https://doi.org/10.1016/j.bej.2023.108900 eng eng fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c doi:10.1016/j.bej.2023.108900 https://avesis.yildiz.edu.tr/publication/details/fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c/oai info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2023 ftyildiztuniv https://doi.org/10.1016/j.bej.2023.108900 2023-08-27T21:20:57Z Studies on pharmaceutical and medicine applications of cold adapted polar microalgae are very limited. In this study, it is aimed to evaluate the cytotoxic activity of polar microalgae extracts against different cancer cell lines. In this context, the cultivation of Chlorella variabilis YTU.ANTARCTIC.001 and Auxenochlorella pyrenoidosa OZCIMEN.001 were carried out and their oil extracts were encapsulated in nanoparticle to preserve its bioactive components against oxidation and to increase the penetration of the extract into the cancer cells. To investigate the anticancer effects of microalgae, cell culture studies were performed and then the extracts and nanoparticles were applied to the AGS and PC-3 cell lines via XTT method. In line with the study, it was observed that C. pyrenoidosa extracts showed higher cytotoxicity than C. variabilis against AGS and PC-3 cells. At 500 µg/ml, AGS cells tested with C. variabilis showed a decrease down to 58 % and at the same concentration of C. pyrenoidosa, it was determined as 51 %. As for nanoparticle form, C. variabilis extracts exhibited higher cytotoxic activity and better anticancer properties. It was found that, these potential anticancer effects appeared to be largely concentration-dependent. Moreover, C. variabilis extract was determined as less effective on scratch closure than C. pyrenoidosa. In conclusion, the obtained data will pave the way to produce biotechnological drugs based on polar microalgae. Article in Journal/Newspaper Antarc* Antarctic Yıldız Technical University Research Information System Antarctic Biochemical Engineering Journal 195 108900 |
institution |
Open Polar |
collection |
Yıldız Technical University Research Information System |
op_collection_id |
ftyildiztuniv |
language |
English |
description |
Studies on pharmaceutical and medicine applications of cold adapted polar microalgae are very limited. In this study, it is aimed to evaluate the cytotoxic activity of polar microalgae extracts against different cancer cell lines. In this context, the cultivation of Chlorella variabilis YTU.ANTARCTIC.001 and Auxenochlorella pyrenoidosa OZCIMEN.001 were carried out and their oil extracts were encapsulated in nanoparticle to preserve its bioactive components against oxidation and to increase the penetration of the extract into the cancer cells. To investigate the anticancer effects of microalgae, cell culture studies were performed and then the extracts and nanoparticles were applied to the AGS and PC-3 cell lines via XTT method. In line with the study, it was observed that C. pyrenoidosa extracts showed higher cytotoxicity than C. variabilis against AGS and PC-3 cells. At 500 µg/ml, AGS cells tested with C. variabilis showed a decrease down to 58 % and at the same concentration of C. pyrenoidosa, it was determined as 51 %. As for nanoparticle form, C. variabilis extracts exhibited higher cytotoxic activity and better anticancer properties. It was found that, these potential anticancer effects appeared to be largely concentration-dependent. Moreover, C. variabilis extract was determined as less effective on scratch closure than C. pyrenoidosa. In conclusion, the obtained data will pave the way to produce biotechnological drugs based on polar microalgae. |
format |
Article in Journal/Newspaper |
author |
Koç, Rabia Çakır Mutlu, Betül İNAN, Benan ÖZÇİMEN, Didem KARACA, Gülcan Ayşin |
spellingShingle |
Koç, Rabia Çakır Mutlu, Betül İNAN, Benan ÖZÇİMEN, Didem KARACA, Gülcan Ayşin Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines |
author_facet |
Koç, Rabia Çakır Mutlu, Betül İNAN, Benan ÖZÇİMEN, Didem KARACA, Gülcan Ayşin |
author_sort |
Koç, Rabia Çakır |
title |
Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines |
title_short |
Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines |
title_full |
Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines |
title_fullStr |
Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines |
title_full_unstemmed |
Bioprospecting Antarctic microalgae as anticancer agent against PC-3 and AGS cell lines |
title_sort |
bioprospecting antarctic microalgae as anticancer agent against pc-3 and ags cell lines |
publishDate |
2023 |
url |
https://avesis.yildiz.edu.tr/publication/details/fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c/oai https://doi.org/10.1016/j.bej.2023.108900 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c doi:10.1016/j.bej.2023.108900 https://avesis.yildiz.edu.tr/publication/details/fa0276a0-44e0-4e30-97e0-d3cf79cb6e3c/oai |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.bej.2023.108900 |
container_title |
Biochemical Engineering Journal |
container_volume |
195 |
container_start_page |
108900 |
_version_ |
1778141655588667392 |