Bioimprinted immobilization of Candida antarctica lipase A for concentration of omega-3 polyunsaturated fatty acids

• Main Authors: Kahveci, D., Xu, X.
• Other Authors: Yeditepe Üniversitesi
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2020
• Subjects: Bioimprinting; Candida antarctica lipase A; Ethanolysis; Immobilization; Omega-3 polyunsaturated fatty acids; Response surface methodology; Bergen; Norway; Antarc*; Antarctica
• Online Access: https://hdl.handle.net/20.500.11831/6979

Bioimprinting and immobilization of Candida antarctica lipase A (CALA) was optimized by response surface methodology to improve its selectivity for concentration of omega-3 polyunsaturated fatty acids (PUFA) by lipase-catalyzed ethanolysis of salmon oil obtained from salmon by-products. Enzyme-to-support ratio, oleic acid concentration, and immobilization time were the factors investigated. Total omega-3 PUFA lost to the ethyl esters (EE) fraction was monitored in relation to the varying levels of the factors. Results showed that the second-order polynomial model generated adequately fitted the experimental data. The most critical factor was the enzyme-tosupport ratio, which led to decreased recovery of omega-3 PUFA in the glycerides fraction with increasing levels of enzyme amount. Optimum conditions were selected as follows: enzyme-to-support ratio, 0.2 (w/w); oleic acid concentration, 50 mM; immobilization time, 85 min. The total omega-3 PUFA lost to the EE fraction through ethanolysis of salmon oil decreased from 3.76 to 1.47 (mol%) under the optimum conditions. © AOCS 2012. Acknowledgments The authors thank Aarhus University and Marine Bioproducts A/S (Bergen, Norway) for the financial support.