Study on the In Vitro Effects of the Mixtures of Polycyclic Aromatic Hydrocarbons (PAHs) and Heavy Metals on Ethoxyresorufin-O-Deethylase (EROD) Activity in Mossambica tilapia Liver

• Main Authors: Han, Da Xiong, Wang, Hai Yan, Yue, Hong Wei, Wang, Su Min, 韩大雄
• Format: Article in Journal/Newspaper
• Language: English
• Published: SPRINGER 2013
• Subjects: ANGUILLA-ANGUILLA L; DAB LIMANDA-LIMANDA; LEAPING MULLET; INHIBITION; RESPONSES; Anguilla anguilla
• Online Access: http://dspace.xmu.edu.cn/handle/2288/93485

National Public Benefit (Ocean) Research Foundation of China [201105013]; Grand Research Foundation of Fujian Province [2011YZ0001-1, 2013J01386]; Scientific Research Foundation of Third Institute of Oceanography, SOA [201221] This paper reports in vitro effects of individual heavy metals (Cd2+, Cu2+ and Hg2+), and PAHs, including benzo[a]pyrene(BaP), indeno[1,2,3-cd]pyrene (IP) and fluoranthene (FL), and their mixtures on ethoxyresorufin-O-deethylase (EROD) activities using a plate-reader method. The results showed that all three metals inhibited EROD activity, while BaP/IP significantly induced the enzyme. However, FL alone decreased EROD activity. Moreover, co-treatment with BaP/IP and heavy metals inhibited PAH-induced EROD activities, while combined exposure to FL and heavy metals induced FL-inhibited EROD activities.