Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea
National Natural Science Foundation of China [40676002, 40930847, 31070442, 41076130, 41076131]; Emphasis Project Fund of National Scientific and Technical Support Plan [2006BAB18B07]; International Science and Technology Cooperation Program [2008DFA20420]; U.S. National Science Foundation [NSF-OPP-...
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POLAR BIOL
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/90576 2023-05-15T15:15:35+02:00 Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea Zeng, Yinxin Zou, Yang Grebmeier, Jacqueline M. He, Jianfeng Zheng, Tianling 郑天凌 2012-01 http://dspace.xmu.edu.cn/handle/2288/90576 en_US eng POLAR BIOL POLAR BIOLOGY, 2012,35(1):117-129 WOS:000297596700010 http://dspace.xmu.edu.cn/handle/2288/90576 http://dx.doi.org/10.1007/s00300-011-1044-8 16S RIBOSOMAL-RNA COMMUNITY COMPOSITION SP NOV BACTERIOPLANKTON COMMUNITY CYTOPHAGA-FLAVOBACTERIA MICROBIAL ECOLOGY MARINE-BACTERIA ORGANIC-MATTER GENE-SEQUENCES ARCTIC-OCEAN Article 2012 ftxiamenuniv 2020-07-21T11:44:25Z National Natural Science Foundation of China [40676002, 40930847, 31070442, 41076130, 41076131]; Emphasis Project Fund of National Scientific and Technical Support Plan [2006BAB18B07]; International Science and Technology Cooperation Program [2008DFA20420]; U.S. National Science Foundation [NSF-OPP-ARC-0454454]; Public Science and Technology Research Funds Projects of Ocean [201105022] Planktonic bacteria are abundant in the Bering Sea. However, very little is known about their diversity and the roles of various bacteria in the ocean. Bacterioplankton diversity in the northern Bering Sea was investigated using a combination of molecular and cultivation-based methods. Community fingerprint analysis using polymerase chain reaction-denaturing gradient gel electrophoresis revealed an apparent difference in the bacterioplankton community composition between sampling locations in the area. The bacterial communities were characterized by two 16S rRNA gene clone libraries for surface and bottom water at shallow station NEC5 (< 60 m in depth) on the continental shelf. Sequences fell into 21 major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta, Gamma, and Delta), Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, Planctomycetes, Verrucomicrobia, Fusobacteria, Chlamydiae, Chloroflexi, Chlorobi, Spirochaetes, Cyanobacteria (or algal chloroplasts), and candidate divisions OP8, OP11, TM6, TM7, and WS3. Significant differences were found between the two clone libraries. Actinobacteria formed the dominant bacterial lineage in both surface and bottom water, and the Alphaproteobacteria was another dominant fraction in surface water. A total of 232 heterotrophic bacterial strains were isolated and 81% showed extracellular proteolytic activity. Phylogenetic analysis revealed that the isolates fell into three bacterial groups, including the Gammaproteobacteria, Actinobacteria, and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. Divergence of bacterial community composition in the northern Bering Sea was mainly characterized by the dominance of Actinobacteria and reflected a bacterial community different from that currently known for marine bacterioplankton communities in other polar regions. Article in Journal/Newspaper Arctic Arctic Ocean Bering Sea Polar Biology Xiamen University Institutional Repository Arctic Arctic Ocean Bering Sea |
institution |
Open Polar |
collection |
Xiamen University Institutional Repository |
op_collection_id |
ftxiamenuniv |
language |
English |
topic |
16S RIBOSOMAL-RNA COMMUNITY COMPOSITION SP NOV BACTERIOPLANKTON COMMUNITY CYTOPHAGA-FLAVOBACTERIA MICROBIAL ECOLOGY MARINE-BACTERIA ORGANIC-MATTER GENE-SEQUENCES ARCTIC-OCEAN |
spellingShingle |
16S RIBOSOMAL-RNA COMMUNITY COMPOSITION SP NOV BACTERIOPLANKTON COMMUNITY CYTOPHAGA-FLAVOBACTERIA MICROBIAL ECOLOGY MARINE-BACTERIA ORGANIC-MATTER GENE-SEQUENCES ARCTIC-OCEAN Zeng, Yinxin Zou, Yang Grebmeier, Jacqueline M. He, Jianfeng Zheng, Tianling 郑天凌 Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea |
topic_facet |
16S RIBOSOMAL-RNA COMMUNITY COMPOSITION SP NOV BACTERIOPLANKTON COMMUNITY CYTOPHAGA-FLAVOBACTERIA MICROBIAL ECOLOGY MARINE-BACTERIA ORGANIC-MATTER GENE-SEQUENCES ARCTIC-OCEAN |
description |
National Natural Science Foundation of China [40676002, 40930847, 31070442, 41076130, 41076131]; Emphasis Project Fund of National Scientific and Technical Support Plan [2006BAB18B07]; International Science and Technology Cooperation Program [2008DFA20420]; U.S. National Science Foundation [NSF-OPP-ARC-0454454]; Public Science and Technology Research Funds Projects of Ocean [201105022] Planktonic bacteria are abundant in the Bering Sea. However, very little is known about their diversity and the roles of various bacteria in the ocean. Bacterioplankton diversity in the northern Bering Sea was investigated using a combination of molecular and cultivation-based methods. Community fingerprint analysis using polymerase chain reaction-denaturing gradient gel electrophoresis revealed an apparent difference in the bacterioplankton community composition between sampling locations in the area. The bacterial communities were characterized by two 16S rRNA gene clone libraries for surface and bottom water at shallow station NEC5 (< 60 m in depth) on the continental shelf. Sequences fell into 21 major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta, Gamma, and Delta), Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, Planctomycetes, Verrucomicrobia, Fusobacteria, Chlamydiae, Chloroflexi, Chlorobi, Spirochaetes, Cyanobacteria (or algal chloroplasts), and candidate divisions OP8, OP11, TM6, TM7, and WS3. Significant differences were found between the two clone libraries. Actinobacteria formed the dominant bacterial lineage in both surface and bottom water, and the Alphaproteobacteria was another dominant fraction in surface water. A total of 232 heterotrophic bacterial strains were isolated and 81% showed extracellular proteolytic activity. Phylogenetic analysis revealed that the isolates fell into three bacterial groups, including the Gammaproteobacteria, Actinobacteria, and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. Divergence of bacterial community composition in the northern Bering Sea was mainly characterized by the dominance of Actinobacteria and reflected a bacterial community different from that currently known for marine bacterioplankton communities in other polar regions. |
format |
Article in Journal/Newspaper |
author |
Zeng, Yinxin Zou, Yang Grebmeier, Jacqueline M. He, Jianfeng Zheng, Tianling 郑天凌 |
author_facet |
Zeng, Yinxin Zou, Yang Grebmeier, Jacqueline M. He, Jianfeng Zheng, Tianling 郑天凌 |
author_sort |
Zeng, Yinxin |
title |
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea |
title_short |
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea |
title_full |
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea |
title_fullStr |
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea |
title_full_unstemmed |
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea |
title_sort |
culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern bering sea |
publisher |
POLAR BIOL |
publishDate |
2012 |
url |
http://dspace.xmu.edu.cn/handle/2288/90576 |
geographic |
Arctic Arctic Ocean Bering Sea |
geographic_facet |
Arctic Arctic Ocean Bering Sea |
genre |
Arctic Arctic Ocean Bering Sea Polar Biology |
genre_facet |
Arctic Arctic Ocean Bering Sea Polar Biology |
op_source |
http://dx.doi.org/10.1007/s00300-011-1044-8 |
op_relation |
POLAR BIOLOGY, 2012,35(1):117-129 WOS:000297596700010 http://dspace.xmu.edu.cn/handle/2288/90576 |
_version_ |
1766345951851577344 |