Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea
NSFC [41106096, 41191021]; SOA project [201105021]; Science and Technology Planning Project of Xiamen, China [3502Z20102017]; State Key Laboratory of Marine Environmental Science at Xiamen University [MELRS1026] The ubiquitous and abundant distribution of ammonia-oxidizing Thaumarchaeota in marine e...
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/88048 2023-05-15T13:51:20+02:00 Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea Hu, Anyi Yang, Zao Yu, Chang-Ping Jiao, Nianzhi 焦念志 2013-Apr 2 http://dspace.xmu.edu.cn/handle/2288/88048 en_US eng PUBLIC LIBRARY SCIENCE PLOS ONE, 2013,8(4) WOS:000317717300171 http://dspace.xmu.edu.cn/handle/2288/88048 http://dx.doi.org/10.1371/journal.pone.0061087 AMMONIA-OXIDIZING ARCHAEA COASTAL SURFACE WATERS CHANGJIANG ESTUARY MESOPHILIC CRENARCHAEOTA SUMMER BACTERIOPLANKTON TEMPORAL DISTRIBUTION ANTARCTIC PENINSULA NITRITE REDUCTASE NORTH-SEA DIVERSITY Article 2013 ftxiamenuniv 2020-07-21T11:42:35Z NSFC [41106096, 41191021]; SOA project [201105021]; Science and Technology Planning Project of Xiamen, China [3502Z20102017]; State Key Laboratory of Marine Environmental Science at Xiamen University [MELRS1026] The ubiquitous and abundant distribution of ammonia-oxidizing Thaumarchaeota in marine environments is now well documented, and their crucial role in the global nitrogen cycle has been highlighted. However, the potential contribution of Thaumarchaeota in the carbon cycle remains poorly understood. Here we present for the first time a seasonal investigation on the shelf region (bathymetry <= 200 m) of the East China Sea (ECS) involving analysis of both thaumarchaeal 16S rRNA and autotrophy-related genes (acetyl-CoA carboxylase gene, accA). Quantitative PCR results clearly showed a higher abundance of thaumarchaeal 16S and accA genes in late-autumn (November) than summer (August), whereas the diversity and community structure of autotrophic Thaumarchaeota showed no statistically significant difference between different seasons as revealed by thaumarchaeal accA gene clone libraries. Phylogenetic analysis indicated that shallow ecotypes dominated the autotrophic Thaumarchaeota in the ECS shelf (86.3% of total sequences), while a novel non-marine thaumarchaeal accA lineage was identified in the Changjiang estuary in summer (when freshwater plumes become larger) but not in autumn, implying that Changjiang freshwater discharge played a certain role in transporting terrestrial microorganisms to the ECS. Multivariate statistical analysis indicated that the biogeography of the autotrophic Thaumarchaeota in the shelf water of the ECS was influenced by complex hydrographic conditions. However, an in silico comparative analysis suggested that the diversity and abundance of the autotrophic Thaumarchaeota might be biased by the 'universal' thaumarchaeal accA gene primers Cren529F/Cren981R since this primer set is likely to miss some members within particular phylogenetic groups. Collectively, this study improved our understanding of the biogeographic patterns of the autotrophic Thaumarchaeota in temperate coastal waters, and suggested that new accA primers with improved coverage and sensitivity across phylogenetic groups are needed to gain a more thorough understanding of the role of the autotrophic Thaumarchaeota in the global carbon cycle. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Xiamen University Institutional Repository Antarctic Antarctic Peninsula |
institution |
Open Polar |
collection |
Xiamen University Institutional Repository |
op_collection_id |
ftxiamenuniv |
language |
English |
topic |
AMMONIA-OXIDIZING ARCHAEA COASTAL SURFACE WATERS CHANGJIANG ESTUARY MESOPHILIC CRENARCHAEOTA SUMMER BACTERIOPLANKTON TEMPORAL DISTRIBUTION ANTARCTIC PENINSULA NITRITE REDUCTASE NORTH-SEA DIVERSITY |
spellingShingle |
AMMONIA-OXIDIZING ARCHAEA COASTAL SURFACE WATERS CHANGJIANG ESTUARY MESOPHILIC CRENARCHAEOTA SUMMER BACTERIOPLANKTON TEMPORAL DISTRIBUTION ANTARCTIC PENINSULA NITRITE REDUCTASE NORTH-SEA DIVERSITY Hu, Anyi Yang, Zao Yu, Chang-Ping Jiao, Nianzhi 焦念志 Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea |
topic_facet |
AMMONIA-OXIDIZING ARCHAEA COASTAL SURFACE WATERS CHANGJIANG ESTUARY MESOPHILIC CRENARCHAEOTA SUMMER BACTERIOPLANKTON TEMPORAL DISTRIBUTION ANTARCTIC PENINSULA NITRITE REDUCTASE NORTH-SEA DIVERSITY |
description |
NSFC [41106096, 41191021]; SOA project [201105021]; Science and Technology Planning Project of Xiamen, China [3502Z20102017]; State Key Laboratory of Marine Environmental Science at Xiamen University [MELRS1026] The ubiquitous and abundant distribution of ammonia-oxidizing Thaumarchaeota in marine environments is now well documented, and their crucial role in the global nitrogen cycle has been highlighted. However, the potential contribution of Thaumarchaeota in the carbon cycle remains poorly understood. Here we present for the first time a seasonal investigation on the shelf region (bathymetry <= 200 m) of the East China Sea (ECS) involving analysis of both thaumarchaeal 16S rRNA and autotrophy-related genes (acetyl-CoA carboxylase gene, accA). Quantitative PCR results clearly showed a higher abundance of thaumarchaeal 16S and accA genes in late-autumn (November) than summer (August), whereas the diversity and community structure of autotrophic Thaumarchaeota showed no statistically significant difference between different seasons as revealed by thaumarchaeal accA gene clone libraries. Phylogenetic analysis indicated that shallow ecotypes dominated the autotrophic Thaumarchaeota in the ECS shelf (86.3% of total sequences), while a novel non-marine thaumarchaeal accA lineage was identified in the Changjiang estuary in summer (when freshwater plumes become larger) but not in autumn, implying that Changjiang freshwater discharge played a certain role in transporting terrestrial microorganisms to the ECS. Multivariate statistical analysis indicated that the biogeography of the autotrophic Thaumarchaeota in the shelf water of the ECS was influenced by complex hydrographic conditions. However, an in silico comparative analysis suggested that the diversity and abundance of the autotrophic Thaumarchaeota might be biased by the 'universal' thaumarchaeal accA gene primers Cren529F/Cren981R since this primer set is likely to miss some members within particular phylogenetic groups. Collectively, this study improved our understanding of the biogeographic patterns of the autotrophic Thaumarchaeota in temperate coastal waters, and suggested that new accA primers with improved coverage and sensitivity across phylogenetic groups are needed to gain a more thorough understanding of the role of the autotrophic Thaumarchaeota in the global carbon cycle. |
format |
Article in Journal/Newspaper |
author |
Hu, Anyi Yang, Zao Yu, Chang-Ping Jiao, Nianzhi 焦念志 |
author_facet |
Hu, Anyi Yang, Zao Yu, Chang-Ping Jiao, Nianzhi 焦念志 |
author_sort |
Hu, Anyi |
title |
Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea |
title_short |
Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea |
title_full |
Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea |
title_fullStr |
Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea |
title_full_unstemmed |
Dynamics of Autotrophic Marine Planktonic Thaumarchaeota in the East China Sea |
title_sort |
dynamics of autotrophic marine planktonic thaumarchaeota in the east china sea |
publisher |
PUBLIC LIBRARY SCIENCE |
publishDate |
2013 |
url |
http://dspace.xmu.edu.cn/handle/2288/88048 |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
http://dx.doi.org/10.1371/journal.pone.0061087 |
op_relation |
PLOS ONE, 2013,8(4) WOS:000317717300171 http://dspace.xmu.edu.cn/handle/2288/88048 |
_version_ |
1766255150208385024 |