Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone
European Community's Seventh Framework Programme [211384]; ANR-AQUAPHAGE from the French Ministry of Science [ANR 07 BDIV 015-06]; ANR-MAORY from the French Ministry of Science [ANR 07 BI.AN 016] The mesopelagic ('twilight') zone of the ocean is the sandwich layer between euphotic sur...
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/87897 2023-05-15T17:36:09+02:00 Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone Weinbauerr, M. G. Liu, J. Motegi, C. Maier, C. Pedrotti, M. L. Dai, M. Gattuso, J. P. 戴民汉 2013 http://dspace.xmu.edu.cn/handle/2288/87897 en_US eng INTER-RESEARCH AQUATIC MICROBIAL ECOLOGY, 2013,71(2):99-115 WOS:000329951800001 http://dspace.xmu.edu.cn/handle/2288/87897 http://dx.doi.org/10.3354/ame01666 NW MEDITERRANEAN SEA GRADIENT GEL-ELECTROPHORESIS NORTH-ATLANTIC BATHYPELAGIC WATERS GROWTH EFFICIENCY MESOPELAGIC ZONE HETEROTROPHIC METABOLISM SAR202 BACTERIOPLANKTON MARINE BACTERIOPLANKTON VIRAL ABUNDANCE Article 2013 ftxiamenuniv 2020-07-21T11:42:30Z European Community's Seventh Framework Programme [211384]; ANR-AQUAPHAGE from the French Ministry of Science [ANR 07 BDIV 015-06]; ANR-MAORY from the French Ministry of Science [ANR 07 BI.AN 016] The mesopelagic ('twilight') zone of the ocean is the sandwich layer between euphotic surface waters and the deep ocean. The physical, chemical, and biological processes in this layer strongly influence the marine carbon cycle, although some surprising knowledge gaps remain, e.g. with respect to microbial communities. We therefore determined biological parameters such as microbial abundances, community respiration, and bacterial and archaeal community composition (as assessed by 16S rRNA gene PCR denaturing gradient gel electrophoresis, DGGE) over a seasonal time scale (at ca. 46 d intervals for 433 d) at a coastal mesopelagic site in the NW Mediterranean Sea (Point C, 300 m depth) and related them to physicochemical parameters. Some chemical and most biological parameters showed strong variability, thus confirming the emerging view that this water layer is more dynamic than previously thought. Community respiration was likely influenced by seasonal changes as indicated by its indirect relationship with water density. The high detected community respiration rates (0.23-1.65 mu mol O-2 l(-1) d(-1)) confirm that the twilight zone plays a critical role as a hub between surface and deep water. Statistical analyses indicate that water stratification, dissolved organic carbon, and transparent exopolymeric particles were controlling factors of bacterial community composition, whereas the archaeal community was likely controlled by other mechanisms. A sequence analysis of DGGE bands and a literature comparison suggests some specificity of the mesopelagic zone regarding bacterial and archaeal community composition, thus further confirming the peculiarity of this water layer. Article in Journal/Newspaper North Atlantic Xiamen University Institutional Repository Sea Point ENVELOPE(-61.481,-61.481,57.517,57.517) |
institution |
Open Polar |
collection |
Xiamen University Institutional Repository |
op_collection_id |
ftxiamenuniv |
language |
English |
topic |
NW MEDITERRANEAN SEA GRADIENT GEL-ELECTROPHORESIS NORTH-ATLANTIC BATHYPELAGIC WATERS GROWTH EFFICIENCY MESOPELAGIC ZONE HETEROTROPHIC METABOLISM SAR202 BACTERIOPLANKTON MARINE BACTERIOPLANKTON VIRAL ABUNDANCE |
spellingShingle |
NW MEDITERRANEAN SEA GRADIENT GEL-ELECTROPHORESIS NORTH-ATLANTIC BATHYPELAGIC WATERS GROWTH EFFICIENCY MESOPELAGIC ZONE HETEROTROPHIC METABOLISM SAR202 BACTERIOPLANKTON MARINE BACTERIOPLANKTON VIRAL ABUNDANCE Weinbauerr, M. G. Liu, J. Motegi, C. Maier, C. Pedrotti, M. L. Dai, M. Gattuso, J. P. 戴民汉 Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
topic_facet |
NW MEDITERRANEAN SEA GRADIENT GEL-ELECTROPHORESIS NORTH-ATLANTIC BATHYPELAGIC WATERS GROWTH EFFICIENCY MESOPELAGIC ZONE HETEROTROPHIC METABOLISM SAR202 BACTERIOPLANKTON MARINE BACTERIOPLANKTON VIRAL ABUNDANCE |
description |
European Community's Seventh Framework Programme [211384]; ANR-AQUAPHAGE from the French Ministry of Science [ANR 07 BDIV 015-06]; ANR-MAORY from the French Ministry of Science [ANR 07 BI.AN 016] The mesopelagic ('twilight') zone of the ocean is the sandwich layer between euphotic surface waters and the deep ocean. The physical, chemical, and biological processes in this layer strongly influence the marine carbon cycle, although some surprising knowledge gaps remain, e.g. with respect to microbial communities. We therefore determined biological parameters such as microbial abundances, community respiration, and bacterial and archaeal community composition (as assessed by 16S rRNA gene PCR denaturing gradient gel electrophoresis, DGGE) over a seasonal time scale (at ca. 46 d intervals for 433 d) at a coastal mesopelagic site in the NW Mediterranean Sea (Point C, 300 m depth) and related them to physicochemical parameters. Some chemical and most biological parameters showed strong variability, thus confirming the emerging view that this water layer is more dynamic than previously thought. Community respiration was likely influenced by seasonal changes as indicated by its indirect relationship with water density. The high detected community respiration rates (0.23-1.65 mu mol O-2 l(-1) d(-1)) confirm that the twilight zone plays a critical role as a hub between surface and deep water. Statistical analyses indicate that water stratification, dissolved organic carbon, and transparent exopolymeric particles were controlling factors of bacterial community composition, whereas the archaeal community was likely controlled by other mechanisms. A sequence analysis of DGGE bands and a literature comparison suggests some specificity of the mesopelagic zone regarding bacterial and archaeal community composition, thus further confirming the peculiarity of this water layer. |
format |
Article in Journal/Newspaper |
author |
Weinbauerr, M. G. Liu, J. Motegi, C. Maier, C. Pedrotti, M. L. Dai, M. Gattuso, J. P. 戴民汉 |
author_facet |
Weinbauerr, M. G. Liu, J. Motegi, C. Maier, C. Pedrotti, M. L. Dai, M. Gattuso, J. P. 戴民汉 |
author_sort |
Weinbauerr, M. G. |
title |
Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
title_short |
Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
title_full |
Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
title_fullStr |
Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
title_full_unstemmed |
Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
title_sort |
seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone |
publisher |
INTER-RESEARCH |
publishDate |
2013 |
url |
http://dspace.xmu.edu.cn/handle/2288/87897 |
long_lat |
ENVELOPE(-61.481,-61.481,57.517,57.517) |
geographic |
Sea Point |
geographic_facet |
Sea Point |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
http://dx.doi.org/10.3354/ame01666 |
op_relation |
AQUATIC MICROBIAL ECOLOGY, 2013,71(2):99-115 WOS:000329951800001 http://dspace.xmu.edu.cn/handle/2288/87897 |
_version_ |
1766135538303107072 |