Induction of gynogenetic diploids in the small abalone, Haliotis diversicolor supertexta

• Main Authors: Cai, MY, Ke, CH, Wang, ZY, Zhou, SQ, Zhang, ZX, Wang, YL, Zhang, ZP, 张中新
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2004
• Subjects: PACIFIC ABALONE; ARTIFICIAL GYNOGENESIS; CRASSOSTREA-GIGAS; IRRADIATED SPERM; CYTOCHALASIN-B; MANIPULATION; OYSTER; PLOIDY; Pacific; Crassostrea gigas
• Online Access: https://dspace.xmu.edu.cn/handle/2288/66181

The conditions for induction of gynogentic haploids and restoration of gynogenetic diploids, were studied in the small abalone, Haliotis diversicolor supertexta. Gynogentic diploids were produced by first using chromosome inactivated sperm to fertilize normal eggs and then suppressing the second meiotic division of these eggs. Sperm chromosomes were effectively inactivated with UV irradiation for 50-90 sec at an intensity of 1075 mu W cm(-2.)s(-1). The decreased capacity of UV irradiated sperm to fertilize eggs could be improved to an acceptable level by increasing insemination concentration of sperm. Eggs fertilized with genetically inactivated sperm developed into abnormal larvae in the haploid state. The diploids were restored when the gynogenetically activated eggs were treated with 0.5 mg l(-1) cytochalasin B (CB) for 10 min from 7 min to 17 min post insemination.