Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea
The "Atama complex", which consists of Alexandrium tamarense, A. fundyense, and A. catenella, is one of the most important groups within the dinoflagellate genus Alexandrium. Information of the biogeography of the Atama complex is limited in the Arctic Ocean. In the present study, we estab...
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/60576 2023-05-15T15:10:14+02:00 Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea Gu, Haifeng Zeng, Ni Xie, Zhangxian Wang, Dazhi Wang, Weiguo Yang, Weidong 王大志 2013-03 http://dspace.xmu.edu.cn/handle/2288/60576 en_US eng Polar Biology, 2013,36(3):427-436 0722-4060 WOS:000314852300012 http://dspace.xmu.edu.cn/handle/2288/60576 http://dx.doi.org/10.1007/s00300-012-1273-5 Alexandrium tamarense Atama complex Chukchi Sea Morphology Phylogeny PSP toxin Article 2013 ftxiamenuniv 2020-07-21T11:32:12Z The "Atama complex", which consists of Alexandrium tamarense, A. fundyense, and A. catenella, is one of the most important groups within the dinoflagellate genus Alexandrium. Information of the biogeography of the Atama complex is limited in the Arctic Ocean. In the present study, we established 55 strains of the Atama complex by incubating ellipsoidal cysts collected from the Chukchi Sea. The vegetative cells are characterized by a prominent ventral pore, thereby fitting the description of A. tamarense morphotype. Large subunit (LSU) and/or internal transcribed spacer (ITS) region sequences of these strains were examined. Both sequences showed intragenomic polymorphism. The 708 bp of the LSU sequences from the strains differed from each other at 0-44 sites (0.0-6.2 %), and the ITS region sequences differed from one another at 0-28 sites (0.0-5.4 %). Phylogenetic analysis revealed that the Chukchi Sea strains were nested within Atama complex (Group I). Assessment of paralytic shellfish poisoning toxin production by four Chukchi Sea strains using high-performance liquid chromatography showed that total toxin per cell ranged from 9 to 41 fmol cell(-1). The toxin profile of the four strains from the Chukchi Sea is conserved, with the major toxins being N-sulfocarbamoyl toxin (C2), saxitoxin, and gonyautoxin-4. Our results support that dispersal of the Atama complex (Group I) from the Bering Sea to the Chukchi Sea might have occurred. Article in Journal/Newspaper Arctic Arctic Ocean Bering Sea Chukchi Chukchi Sea Polar Biology Xiamen University Institutional Repository Arctic Arctic Ocean Bering Sea Chukchi Sea |
institution |
Open Polar |
collection |
Xiamen University Institutional Repository |
op_collection_id |
ftxiamenuniv |
language |
English |
topic |
Alexandrium tamarense Atama complex Chukchi Sea Morphology Phylogeny PSP toxin |
spellingShingle |
Alexandrium tamarense Atama complex Chukchi Sea Morphology Phylogeny PSP toxin Gu, Haifeng Zeng, Ni Xie, Zhangxian Wang, Dazhi Wang, Weiguo Yang, Weidong 王大志 Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea |
topic_facet |
Alexandrium tamarense Atama complex Chukchi Sea Morphology Phylogeny PSP toxin |
description |
The "Atama complex", which consists of Alexandrium tamarense, A. fundyense, and A. catenella, is one of the most important groups within the dinoflagellate genus Alexandrium. Information of the biogeography of the Atama complex is limited in the Arctic Ocean. In the present study, we established 55 strains of the Atama complex by incubating ellipsoidal cysts collected from the Chukchi Sea. The vegetative cells are characterized by a prominent ventral pore, thereby fitting the description of A. tamarense morphotype. Large subunit (LSU) and/or internal transcribed spacer (ITS) region sequences of these strains were examined. Both sequences showed intragenomic polymorphism. The 708 bp of the LSU sequences from the strains differed from each other at 0-44 sites (0.0-6.2 %), and the ITS region sequences differed from one another at 0-28 sites (0.0-5.4 %). Phylogenetic analysis revealed that the Chukchi Sea strains were nested within Atama complex (Group I). Assessment of paralytic shellfish poisoning toxin production by four Chukchi Sea strains using high-performance liquid chromatography showed that total toxin per cell ranged from 9 to 41 fmol cell(-1). The toxin profile of the four strains from the Chukchi Sea is conserved, with the major toxins being N-sulfocarbamoyl toxin (C2), saxitoxin, and gonyautoxin-4. Our results support that dispersal of the Atama complex (Group I) from the Bering Sea to the Chukchi Sea might have occurred. |
format |
Article in Journal/Newspaper |
author |
Gu, Haifeng Zeng, Ni Xie, Zhangxian Wang, Dazhi Wang, Weiguo Yang, Weidong 王大志 |
author_facet |
Gu, Haifeng Zeng, Ni Xie, Zhangxian Wang, Dazhi Wang, Weiguo Yang, Weidong 王大志 |
author_sort |
Gu, Haifeng |
title |
Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea |
title_short |
Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea |
title_full |
Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea |
title_fullStr |
Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea |
title_full_unstemmed |
Morphology, phylogeny, and toxicity of Atama complex (Dinophyceae) from the Chukchi Sea |
title_sort |
morphology, phylogeny, and toxicity of atama complex (dinophyceae) from the chukchi sea |
publishDate |
2013 |
url |
http://dspace.xmu.edu.cn/handle/2288/60576 |
geographic |
Arctic Arctic Ocean Bering Sea Chukchi Sea |
geographic_facet |
Arctic Arctic Ocean Bering Sea Chukchi Sea |
genre |
Arctic Arctic Ocean Bering Sea Chukchi Chukchi Sea Polar Biology |
genre_facet |
Arctic Arctic Ocean Bering Sea Chukchi Chukchi Sea Polar Biology |
op_source |
http://dx.doi.org/10.1007/s00300-012-1273-5 |
op_relation |
Polar Biology, 2013,36(3):427-436 0722-4060 WOS:000314852300012 http://dspace.xmu.edu.cn/handle/2288/60576 |
_version_ |
1766341281272823808 |