南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析
南极等极端环境中蕴藏着丰富的资源,其中微生物资源是国内外研究的重点。它们经过长期的进化、选择,在各种极端的环境条件下形成了独特的组织结构、酶系统及代谢机制以进行生存和繁衍。 本文首先从南极中山站排污入海口(ZZS)沉积物样品中直接提取环境样品微生物总DNA,构建了ZSFosmid宏基因组文库。然后采用了NIPAB滤纸法从该文库筛选到了一个能够分泌青霉素酰化酶的阳性克隆子。DNA测序后分析表明该克隆子包含了一个由724个氨基酸编码组成的蛋白质的完整的开放阅读框(ORF)。NCBI数据库比对分析该克隆子包含一个完整的青霉素酰化酶基因,大小为2172bp,预测蛋白质的分子量为80.435kD。随后....
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/53493 2023-05-15T13:32:05+02:00 南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 The construction of metagenomic library from the sediment of the Zhongshan Station,antarctica and the clone,expression and analysis the characterization of penicillin amidase ACPGA001 张千 蔡立哲 曾润颖 2011-03-01 16:16:36.0 http://dspace.xmu.edu.cn/handle/2288/53493 zh_CN chi http://210.34.4.28/opac/openlink.php?strText=27116&doctype=ALL&strSearchType=callno http://dspace.xmu.edu.cn/handle/2288/53493 http://210.34.4.13:8080/lunwen/detail.asp?serial=27907 metagenome penicillin amidase agarase 宏基因组 青霉素酰化酶 琼胶酶 thesis 2011 ftxiamenuniv 2020-07-21T12:17:44Z 南极等极端环境中蕴藏着丰富的资源,其中微生物资源是国内外研究的重点。它们经过长期的进化、选择,在各种极端的环境条件下形成了独特的组织结构、酶系统及代谢机制以进行生存和繁衍。 本文首先从南极中山站排污入海口(ZZS)沉积物样品中直接提取环境样品微生物总DNA,构建了ZSFosmid宏基因组文库。然后采用了NIPAB滤纸法从该文库筛选到了一个能够分泌青霉素酰化酶的阳性克隆子。DNA测序后分析表明该克隆子包含了一个由724个氨基酸编码组成的蛋白质的完整的开放阅读框(ORF)。NCBI数据库比对分析该克隆子包含一个完整的青霉素酰化酶基因,大小为2172bp,预测蛋白质的分子量为80.435kD。随后. In this study, we constructed a metagenomic Fosmid library from a sediment sample collected from the coast near the Zhangshan Station (ZSS), Antarctica. A clone producing penicillin amidase was isolated form this library. This clone was found to contain an ORF encoded a protein of 724 amino acids that represented typical features of a penicillin amidase. This penicillin amidase gene was cloned int. 学位:理学硕士 院系专业:海洋与环境学院环境科学与工程系_环境科学 学号:22620071152888 Thesis Antarc* Antarctica Xiamen University Institutional Repository Zhongshan ENVELOPE(76.371,76.371,-69.373,-69.373) Zhongshan Station ENVELOPE(76.371,76.371,-69.373,-69.373) |
institution |
Open Polar |
collection |
Xiamen University Institutional Repository |
op_collection_id |
ftxiamenuniv |
language |
Chinese |
topic |
metagenome penicillin amidase agarase 宏基因组 青霉素酰化酶 琼胶酶 |
spellingShingle |
metagenome penicillin amidase agarase 宏基因组 青霉素酰化酶 琼胶酶 张千 南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 |
topic_facet |
metagenome penicillin amidase agarase 宏基因组 青霉素酰化酶 琼胶酶 |
description |
南极等极端环境中蕴藏着丰富的资源,其中微生物资源是国内外研究的重点。它们经过长期的进化、选择,在各种极端的环境条件下形成了独特的组织结构、酶系统及代谢机制以进行生存和繁衍。 本文首先从南极中山站排污入海口(ZZS)沉积物样品中直接提取环境样品微生物总DNA,构建了ZSFosmid宏基因组文库。然后采用了NIPAB滤纸法从该文库筛选到了一个能够分泌青霉素酰化酶的阳性克隆子。DNA测序后分析表明该克隆子包含了一个由724个氨基酸编码组成的蛋白质的完整的开放阅读框(ORF)。NCBI数据库比对分析该克隆子包含一个完整的青霉素酰化酶基因,大小为2172bp,预测蛋白质的分子量为80.435kD。随后. In this study, we constructed a metagenomic Fosmid library from a sediment sample collected from the coast near the Zhangshan Station (ZSS), Antarctica. A clone producing penicillin amidase was isolated form this library. This clone was found to contain an ORF encoded a protein of 724 amino acids that represented typical features of a penicillin amidase. This penicillin amidase gene was cloned int. 学位:理学硕士 院系专业:海洋与环境学院环境科学与工程系_环境科学 学号:22620071152888 |
author2 |
蔡立哲 曾润颖 |
format |
Thesis |
author |
张千 |
author_facet |
张千 |
author_sort |
张千 |
title |
南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 |
title_short |
南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 |
title_full |
南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 |
title_fullStr |
南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 |
title_full_unstemmed |
南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因ACPGA001的克隆、表达和性质分析 |
title_sort |
南极中山站沉积物宏基因组文库的构建及青霉素酰化酶基因acpga001的克隆、表达和性质分析 |
publishDate |
2011 |
url |
http://dspace.xmu.edu.cn/handle/2288/53493 |
long_lat |
ENVELOPE(76.371,76.371,-69.373,-69.373) ENVELOPE(76.371,76.371,-69.373,-69.373) |
geographic |
Zhongshan Zhongshan Station |
geographic_facet |
Zhongshan Zhongshan Station |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
http://210.34.4.13:8080/lunwen/detail.asp?serial=27907 |
op_relation |
http://210.34.4.28/opac/openlink.php?strText=27116&doctype=ALL&strSearchType=callno http://dspace.xmu.edu.cn/handle/2288/53493 |
_version_ |
1766024081930452992 |