大黄鱼免疫相关基因的筛选和两种石首鱼RPL10/QM-like基因特性研究

• Main Author: 陈心
• Other Authors: 苏永全
• Format: Thesis
• Language: Chinese
• Published: 2011
• Subjects: 大黄鱼(Pseudosciaena crocea); 眼斑拟石首鱼(Sciaenops ocellatus); 基因芯片; RPL10/QM-like; Pseudosciaena crocea; Sciaenops ocellatus; Microarray
• Online Access: http://dspace.xmu.edu.cn/handle/2288/53298

本研究以石首鱼科大黄鱼(Pseudosciaenacrocea)为研究对象，利用基因芯片和Real-time等技术筛选了大黄鱼免疫相关基因，研究了大黄鱼核糖体蛋白L10(RPL10/QM-like)基因及其表达产物，同时研究了大黄鱼亲缘种眼斑拟石首鱼(Sciaenopsocellatus)的RPL10/QM-like基因，研究结果充实了石首鱼类免疫学研究内容，也为鱼类的进化研究提供有价值的参考资料。主要结果如下： 1、 病原菌的鉴定 本研究从患病中华乌塘鳢中分离出两种病原菌，经过回归感染，再次分离，分别从菌落形态、生理生化水平和分子生物学水平，分析鉴定该两种菌。鉴定结果表明此两种菌株分别为. In this study, the immuno-related genes from Pseudosciaena crocea were scanned using microassay and real-time PCR method and studied the ribosomal protein L10 (RPL10/QM-like) gene and its product from P. crocea, which provide theoretical basis for studying the immunity of the sciaenoid. At the same time, RPL10/QM-like gene from Sciaenops ocellatus was sequenced and compared with other fish to prov. 学位：理学博士 院系专业：海洋与环境学院海洋学系_海洋生物学 学号：22420060153338