Immune Response of Turbot to Polyriboinosinic-polyribocytidilic Acid: the Transcription of Mx, Hepcidin, and IgM

• Published in: Journal of the World Aquaculture Society
• Main Authors: Mao, Mingguang, 毛明光, Hong, Wanshu, 洪万树, Wang, Kejian, 王克坚, Liu, Zongzhu, Lei, Jilin
• Format: Article in Journal/Newspaper
• Language: English
• Published: WILEY-BLACKWELL 2012
• Subjects: SALMO-SALAR L; TOLL-LIKE RECEPTOR-3; REPORTER GENE ASSAY; DOUBLE-STRANDED-RNA; PROTEIN EXPRESSION; I INTERFERON; PARALICHTHYS-OLIVACEUS; SCOPHTHALMUS-MAXIMUS; DIETARY NUCLEOTIDES; ONCORHYNCHUS-MYKISS; Salmo salar; Scophthalmus maximus; Turbot
• Online Access: https://doi.org/10.1111/j.1749-7345.2012.00559.x; http://dspace.xmu.edu.cn/handle/2288/15620

Polyriboinosinic-polyribocytidilic acid (poly I:C) was administered to turbot, Scophthalmus maximus, via injection, oral route, and immersion. Samples of liver, spleen, and head kidney were individually collected at different time intervals (0, 24, 48, 72, 96, and 120 h) after poly I:C treatments. Transcription levels of Mx, hepcidin, and IgM in the organs of turbot were detected using RT-PCR. It was found that Mx mRNA was significantly induced in the liver, head kidney, and spleen 48-h postinjection with poly I:C, and the effects of immersion and oral route on Mx transcription were analogous to that of injection method. Meanwhile, the hepcidin mRNA was significantly induced in the spleen but not in the liver and head kidney 48-h postinjection. No significant increase of IgM mRNA was found in the liver and spleen after poly I:C challenge. The results indicated that the antivirus state resulted from the Mx expression and could be induced with poly I:C via injection, immersion, and oral methods. The influence of poly I:C on IgM and hepcidin transcription suggested that both innate and adaptive immunity could be modulated through interferon-inducible signaling pathways. Emarked Fund for Modern Agro-industry Technology Research System [nycytx-50]; Qingdao Key Laboratory for Marine Fish Breeding and Biology