Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis
A method for determining iron in seawater had been developed by coupling reverse flow injection analysis (rFIA) and catalytic spectrophotometric detection with N,N-dimethyl-p-phenylenediamine dihydrochloride (DPD). With a seawater sample or a standard solution as the carrier, the mixture of DPD and...
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/15456 2023-05-15T17:52:08+02:00 Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis Huang, Yongming Yuan, Dongxing 袁东星 Dai, Minhan 戴民汉 Liu, Yaoxing 2012-05-15 https://doi.org/10.1016/j.talanta.2012.01.050 http://dspace.xmu.edu.cn/handle/2288/15456 en eng ELSEVIER SCIENCE BV TALANTA,2012,93:86-93 0039-9140 http://dx.doi.org/10.1016/j.talanta.2012.01.050 WOS:000303305700012 http://dspace.xmu.edu.cn/handle/2288/15456 Reverse flow injection analysis Catalytic spectrophotometry Iron Estuarine and coastal waters The Pearl River Estuary Article 2012 ftxiamenuniv https://doi.org/10.1016/j.talanta.2012.01.050 2020-07-21T11:24:54Z A method for determining iron in seawater had been developed by coupling reverse flow injection analysis (rFIA) and catalytic spectrophotometric detection with N,N-dimethyl-p-phenylenediamine dihydrochloride (DPD). With a seawater sample or a standard solution as the carrier, the mixture of DPD and buffer was injected into the carrier stream quantitatively and discretely. After mixing with H2O2, the DPD was oxidized to form two pink semiquinone derivatives that were monitored at 514 nm wavelength with a reference at 700 nm. The method detection limit was 0.40 nmol L-1, lower than half of that of normal flow injection analysis (nFIA) method. The sample throughput was 10 h(-1) with triplicate determination, compared with 4 h(-1) for nFIA-DPD method. The analysis results of the certified seawaters CASS-4 (12.33 +/- 0.18 nmol L-1) and NASS-5 (3.47 +/- 0.23 nmol L-1) well agreed with the certified values (12.77 +/- 1.04 and 3.71 +/- 0.63 nmol L-1, respectively). The typical precision of the method for a 2.97 nmol L-1 iron sample was 4.49% (n = 8). Interferences from copper and salinity were investigated. An instrument was assembled based on the proposed method and applied successfully to analyze total dissolvable iron (TDFe) in surface seawater samples collected from the Pearl River Estuary, the results of which revealed non-conservative behavior of TDFe during the estuarine mixing. Results for these samples with both rFIA-DPD and nFIA-DPD methods showed good agreement with each other. The proposed method was superior to the currently used nFIA-DPD method, particularly when it is adapted for field and in situ deployment, due to its lower reagent consumption, higher sample throughput and keeping the manifold tubing clean. (C) 2012 Elsevier B.V. All rights reserved. China National Basic Research Program ("973"Program) Carbon cycling in China Seas-budget, controls and ocean acidification (CHOICE-C) [2009CB421200]; State Key Laboratory of Marine Environmental Science at Xiamen University through MEL [MELRI0703] Article in Journal/Newspaper Ocean acidification Xiamen University Institutional Repository Talanta 93 86 93 |
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Xiamen University Institutional Repository |
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ftxiamenuniv |
language |
English |
topic |
Reverse flow injection analysis Catalytic spectrophotometry Iron Estuarine and coastal waters The Pearl River Estuary |
spellingShingle |
Reverse flow injection analysis Catalytic spectrophotometry Iron Estuarine and coastal waters The Pearl River Estuary Huang, Yongming Yuan, Dongxing 袁东星 Dai, Minhan 戴民汉 Liu, Yaoxing Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis |
topic_facet |
Reverse flow injection analysis Catalytic spectrophotometry Iron Estuarine and coastal waters The Pearl River Estuary |
description |
A method for determining iron in seawater had been developed by coupling reverse flow injection analysis (rFIA) and catalytic spectrophotometric detection with N,N-dimethyl-p-phenylenediamine dihydrochloride (DPD). With a seawater sample or a standard solution as the carrier, the mixture of DPD and buffer was injected into the carrier stream quantitatively and discretely. After mixing with H2O2, the DPD was oxidized to form two pink semiquinone derivatives that were monitored at 514 nm wavelength with a reference at 700 nm. The method detection limit was 0.40 nmol L-1, lower than half of that of normal flow injection analysis (nFIA) method. The sample throughput was 10 h(-1) with triplicate determination, compared with 4 h(-1) for nFIA-DPD method. The analysis results of the certified seawaters CASS-4 (12.33 +/- 0.18 nmol L-1) and NASS-5 (3.47 +/- 0.23 nmol L-1) well agreed with the certified values (12.77 +/- 1.04 and 3.71 +/- 0.63 nmol L-1, respectively). The typical precision of the method for a 2.97 nmol L-1 iron sample was 4.49% (n = 8). Interferences from copper and salinity were investigated. An instrument was assembled based on the proposed method and applied successfully to analyze total dissolvable iron (TDFe) in surface seawater samples collected from the Pearl River Estuary, the results of which revealed non-conservative behavior of TDFe during the estuarine mixing. Results for these samples with both rFIA-DPD and nFIA-DPD methods showed good agreement with each other. The proposed method was superior to the currently used nFIA-DPD method, particularly when it is adapted for field and in situ deployment, due to its lower reagent consumption, higher sample throughput and keeping the manifold tubing clean. (C) 2012 Elsevier B.V. All rights reserved. China National Basic Research Program ("973"Program) Carbon cycling in China Seas-budget, controls and ocean acidification (CHOICE-C) [2009CB421200]; State Key Laboratory of Marine Environmental Science at Xiamen University through MEL [MELRI0703] |
format |
Article in Journal/Newspaper |
author |
Huang, Yongming Yuan, Dongxing 袁东星 Dai, Minhan 戴民汉 Liu, Yaoxing |
author_facet |
Huang, Yongming Yuan, Dongxing 袁东星 Dai, Minhan 戴民汉 Liu, Yaoxing |
author_sort |
Huang, Yongming |
title |
Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis |
title_short |
Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis |
title_full |
Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis |
title_fullStr |
Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis |
title_full_unstemmed |
Reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: A comparison with normal flow injection analysis |
title_sort |
reverse flow injection analysis method for catalytic spectrophotometric determination of iron in estuarine and coastal waters: a comparison with normal flow injection analysis |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2012 |
url |
https://doi.org/10.1016/j.talanta.2012.01.050 http://dspace.xmu.edu.cn/handle/2288/15456 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
TALANTA,2012,93:86-93 0039-9140 http://dx.doi.org/10.1016/j.talanta.2012.01.050 WOS:000303305700012 http://dspace.xmu.edu.cn/handle/2288/15456 |
op_doi |
https://doi.org/10.1016/j.talanta.2012.01.050 |
container_title |
Talanta |
container_volume |
93 |
container_start_page |
86 |
op_container_end_page |
93 |
_version_ |
1766159495087521792 |