A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol
The kinetics and thermodynamics of Candida antarctica lipase B-catalyzed remote asymmetric alcoholysis of the citalopram intermediate 3-[(acetyloxy)methyl]-4-[4-(dimethylamino)-1-(4-fluorophenyl)-1-hydroxybutyl]-benzonitrile (diol monoacetate) have been studied. A kinetic model based on the reversib...
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ftxiamenuniv:oai:dspace.xmu.edu.cn:2288/15258 2023-05-15T14:00:22+02:00 A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol Wang, Shi-Zhen 王世珍 Wu, Jian-Ping Xu, Gang Yang, Li-Rong 2012-06-15 https://doi.org/10.1016/j.bej.2012.04.005 http://dspace.xmu.edu.cn/handle/2288/15258 en eng ELSEVIER SCIENCE SA BIOCHEMICAL ENGINEERING JOURNAL,2012,65:57-62 1369-703X http://dx.doi.org/10.1016/j.bej.2012.04.005 WOS:000305300400009 http://dspace.xmu.edu.cn/handle/2288/15258 Dynamic modeling Kinetic parameters Lipase Immobilized enzymes Tertiary alcohol Asymmetric alcoholysis Article 2012 ftxiamenuniv https://doi.org/10.1016/j.bej.2012.04.005 2020-07-21T11:24:46Z The kinetics and thermodynamics of Candida antarctica lipase B-catalyzed remote asymmetric alcoholysis of the citalopram intermediate 3-[(acetyloxy)methyl]-4-[4-(dimethylamino)-1-(4-fluorophenyl)-1-hydroxybutyl]-benzonitrile (diol monoacetate) have been studied. A kinetic model based on the reversible ping-pong bi-bi mechanism with competitive enantiomer substrates was proposed. The product inhibition by each diol enantiomer and the substrate inhibition by isobutyl alcohol were also considered. The diffusion limitation was proven to be negligible. By reducing the degree of freedom in parameter estimation by model discrimination and Haldane equations, 14 free parameters were successfully identified. The model parameters were simulated by the Matlab program using time-concentration curves of different diol monoacetate concentrations; the simulated values fit the experimental values well, with an average relative error of 9.6%. The reaction activity and enantioselectivity of C. antarctica lipase B toward the tertiary alcohol were investigated by kinetic and thermodynamic analysis using simulated kinetic parameters. (c) 2012 Elsevier B.V. All rights reserved. National Natural Science Foundation of China [20936002]; Key Project of Chinese National Programs for Fundamental Research and Development [2011CB710800]; Hi-Tech Research and Development Program of China [2011AA02A209] Article in Journal/Newspaper Antarc* Antarctica Xiamen University Institutional Repository Biochemical Engineering Journal 65 57 62 |
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Open Polar |
collection |
Xiamen University Institutional Repository |
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ftxiamenuniv |
language |
English |
topic |
Dynamic modeling Kinetic parameters Lipase Immobilized enzymes Tertiary alcohol Asymmetric alcoholysis |
spellingShingle |
Dynamic modeling Kinetic parameters Lipase Immobilized enzymes Tertiary alcohol Asymmetric alcoholysis Wang, Shi-Zhen 王世珍 Wu, Jian-Ping Xu, Gang Yang, Li-Rong A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
topic_facet |
Dynamic modeling Kinetic parameters Lipase Immobilized enzymes Tertiary alcohol Asymmetric alcoholysis |
description |
The kinetics and thermodynamics of Candida antarctica lipase B-catalyzed remote asymmetric alcoholysis of the citalopram intermediate 3-[(acetyloxy)methyl]-4-[4-(dimethylamino)-1-(4-fluorophenyl)-1-hydroxybutyl]-benzonitrile (diol monoacetate) have been studied. A kinetic model based on the reversible ping-pong bi-bi mechanism with competitive enantiomer substrates was proposed. The product inhibition by each diol enantiomer and the substrate inhibition by isobutyl alcohol were also considered. The diffusion limitation was proven to be negligible. By reducing the degree of freedom in parameter estimation by model discrimination and Haldane equations, 14 free parameters were successfully identified. The model parameters were simulated by the Matlab program using time-concentration curves of different diol monoacetate concentrations; the simulated values fit the experimental values well, with an average relative error of 9.6%. The reaction activity and enantioselectivity of C. antarctica lipase B toward the tertiary alcohol were investigated by kinetic and thermodynamic analysis using simulated kinetic parameters. (c) 2012 Elsevier B.V. All rights reserved. National Natural Science Foundation of China [20936002]; Key Project of Chinese National Programs for Fundamental Research and Development [2011CB710800]; Hi-Tech Research and Development Program of China [2011AA02A209] |
format |
Article in Journal/Newspaper |
author |
Wang, Shi-Zhen 王世珍 Wu, Jian-Ping Xu, Gang Yang, Li-Rong |
author_facet |
Wang, Shi-Zhen 王世珍 Wu, Jian-Ping Xu, Gang Yang, Li-Rong |
author_sort |
Wang, Shi-Zhen |
title |
A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
title_short |
A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
title_full |
A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
title_fullStr |
A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
title_full_unstemmed |
A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
title_sort |
kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol |
publisher |
ELSEVIER SCIENCE SA |
publishDate |
2012 |
url |
https://doi.org/10.1016/j.bej.2012.04.005 http://dspace.xmu.edu.cn/handle/2288/15258 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
BIOCHEMICAL ENGINEERING JOURNAL,2012,65:57-62 1369-703X http://dx.doi.org/10.1016/j.bej.2012.04.005 WOS:000305300400009 http://dspace.xmu.edu.cn/handle/2288/15258 |
op_doi |
https://doi.org/10.1016/j.bej.2012.04.005 |
container_title |
Biochemical Engineering Journal |
container_volume |
65 |
container_start_page |
57 |
op_container_end_page |
62 |
_version_ |
1766269431725424640 |