Lipases are soluble and active in glycerol carbonate as a novel biosolvent

Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity i...

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Bibliographic Details
Published in:Enzyme and Microbial Technology
Main Authors: Ou, Guangnan（Jimei Univ, Sch Bioengn）, He, Biyan（Jimei Univ, Sch Bioengn）, Yuan, Youzhu, 袁友珠
Format: Article in Journal/Newspaper
Language:English
Published: ELSEVIER SCIENCE INC 2011
Subjects:
Biosolvent
Ionization
Dissociation
Lipase
Activity
Rugosa
Antarc*
Antarctica
Online Access:https://doi.org/10.1016/j.enzmictec.2011.04.011
http://dspace.xmu.edu.cn/handle/2288/11583
Description
Summary:Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity in glycerol carbonate was compared with that in water and in conventional organic solvents with different ionizing and dissociating abilities. The pK(a) value of trichloroacetic acid and transesterification activities of Candida antarctica lipase B and Candida rugosa lipase in glycerol carbonate are similar to those in water, indicating that ionizing and dissociating powers are capable of satisfactorily predicting the biocompatibility of organic solvents for soluble enzymatic systems. (C) 2011 Elsevier Inc. All rights reserved. NSFC[20873108, 20923004]; Science Foundation of Jimei University; NSF of Fujian Province[2006J0262, E0720002]; National Basic Research Program of China[2009CB939804]

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