Community composition of the marine bacterioplankton in Kongsfjorden (Spitsbergen) as revealed by 16S rRNA gene analysis

• Published in: Polar Biology
• Main Authors: Zheng, Tianling, 郑天凌, Zeng, Yinxin, Li, Huirong
• Format: Article in Journal/Newspaper
• Language: English
• Published: SPRINGER 2009
• Subjects: Biodiversity; Bacterioplankton; Denaturing gradient gel electrophoresis; Clone library; Spitsbergen; International Polar Year; IPY; Kongsfjord*; Kongsfjorden; Polar Biology
• Online Access: https://doi.org/10.1007/s00300-009-0641-2; http://dspace.xmu.edu.cn/handle/2288/10412

Phylogenetic diversity of the marine bacterioplankton in Kongsfjorden (Spitsbergen) was investigated by 16S rRNA gene analysis. Community fingerprint analysis by PCR-denaturing gradient gel electrophoresis revealed that there was no apparent difference of bacterioplankton community composition between sampling locations in the fjord. A higher biodiversity was observed in bottom water of station 3 in the central part of the fjord. By 16S rRNA gene clone library analysis, sequences detected both in surface and bottom water of station 3 fell into eight putative divisions, including Proteobacteria (Alpha, Beta, Gamma and Delta), Bacteroidetes, Actinobacteria, Verrucomicrobia and unidentified bacteria, in addition to chloroplasts of algae. Sequences representing Planctomycetes were only observed in bottom water. Compared to the preponderance of clones representing Gammaproteobacteria (36.5%) and Alphaproteobacteria (29.4%) in bottom water, Alphaproteobacteria (43.6%) and algae (27.7%) constituted two dominant fractions in surface water. Cloned sequences showed 82.1-100% similarity to those described sequences. It suggests that, attributing to the influence of ocean currents as well as freshwater input in the summer, the bacterial community in Kongsfjorden may consist of a mixture of cosmopolitan and uniquely endemic phylotypes. National Natural Science Foundation of China [40676002, 40876097]; China's Action Plan for the International Polar Year (IPY)