An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds

Abstract Optimised methods, developed in this novel study, were utilised throughout the research. In environmental studies of Newfoundland and Ireland, interspecies and spatial variations in the total protein content and polyphenol levels of seaweeds were demonstrated. A positive correlation between...

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Main Author: Ryan, Siobhan
Format: Thesis
Language:English
Published: 2010
Subjects:
Online Access:https://repository.wit.ie/1654/
https://repository.wit.ie/1654/1/Siobhan_Ryan_Thesis.pdf
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spelling ftwit:oai:repository.wit.ie:1654 2023-11-05T03:43:34+01:00 An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds Ryan, Siobhan 2010-10 application/pdf https://repository.wit.ie/1654/ https://repository.wit.ie/1654/1/Siobhan_Ryan_Thesis.pdf en eng https://repository.wit.ie/1654/1/Siobhan_Ryan_Thesis.pdf Ryan, Siobhan (2010) An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds. PhD thesis, Waterford Institute of Technology. *NONE OF THESE* Thesis NonPeerReviewed 2010 ftwit 2023-10-07T16:47:40Z Abstract Optimised methods, developed in this novel study, were utilised throughout the research. In environmental studies of Newfoundland and Ireland, interspecies and spatial variations in the total protein content and polyphenol levels of seaweeds were demonstrated. A positive correlation between total protein and temperature was established. A downstream increase in polyphenol levels of seaweeds was observed and correlated with a downstream increase in seawater salinity. P. lanosa contained the highest levels of all metals. Levels of Pb2+, Cr3+ and Cu2+ in seaweed were highest for sites in Waterford City. The highest proportions of Pb2+, As3+, Cr3+, Cu2+, Ni2+ and Al3+ were intracellular. Baseline levels of total protein, extracted protein and polyphenols of four different seaweed species varied significantly. P. lanosa contained the highest total protein. F. vesiculosus and A. nodosum yielded the highest levels of polyphenols. Significant interspecies variations in total and intracellular metals were observed. Zn2+, As3+ and Mn2+ were dominant for all species. P. lanosa demonstrated the best overall metal bioaccumulation potential. Regression analysis demonstrated correlations between total protein and Pb2+, Ni2+, Cu2+ and Cd2+. Seasonal variations in levels of Cd2+, total protein, extracted protein and polyphenols of P. lanosa were observed. The highest levels of total protein were yielded in May. February demonstrated the highest extracted protein and November the lowest polyphenols. Increases in protein concentration following Cd2+ exposure were observed for P. lanosa sampled in February. Increases in polyphenol levels following Cd2+ exposure were observed in the November samples. Potentiometric titrations and FTIR analysis demonstrated seasonal variations in the binding potential of P. lanosa. Gel filtration chromatography, HPLC analysis and SDS PAGE demonstrated changes to the molecular weights of protein derived from P. lanosa following heavy metal exposure. Thesis Newfoundland Waterford Institute of Technology: WIT Institutional Repository
institution Open Polar
collection Waterford Institute of Technology: WIT Institutional Repository
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language English
topic *NONE OF THESE*
spellingShingle *NONE OF THESE*
Ryan, Siobhan
An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds
topic_facet *NONE OF THESE*
description Abstract Optimised methods, developed in this novel study, were utilised throughout the research. In environmental studies of Newfoundland and Ireland, interspecies and spatial variations in the total protein content and polyphenol levels of seaweeds were demonstrated. A positive correlation between total protein and temperature was established. A downstream increase in polyphenol levels of seaweeds was observed and correlated with a downstream increase in seawater salinity. P. lanosa contained the highest levels of all metals. Levels of Pb2+, Cr3+ and Cu2+ in seaweed were highest for sites in Waterford City. The highest proportions of Pb2+, As3+, Cr3+, Cu2+, Ni2+ and Al3+ were intracellular. Baseline levels of total protein, extracted protein and polyphenols of four different seaweed species varied significantly. P. lanosa contained the highest total protein. F. vesiculosus and A. nodosum yielded the highest levels of polyphenols. Significant interspecies variations in total and intracellular metals were observed. Zn2+, As3+ and Mn2+ were dominant for all species. P. lanosa demonstrated the best overall metal bioaccumulation potential. Regression analysis demonstrated correlations between total protein and Pb2+, Ni2+, Cu2+ and Cd2+. Seasonal variations in levels of Cd2+, total protein, extracted protein and polyphenols of P. lanosa were observed. The highest levels of total protein were yielded in May. February demonstrated the highest extracted protein and November the lowest polyphenols. Increases in protein concentration following Cd2+ exposure were observed for P. lanosa sampled in February. Increases in polyphenol levels following Cd2+ exposure were observed in the November samples. Potentiometric titrations and FTIR analysis demonstrated seasonal variations in the binding potential of P. lanosa. Gel filtration chromatography, HPLC analysis and SDS PAGE demonstrated changes to the molecular weights of protein derived from P. lanosa following heavy metal exposure.
format Thesis
author Ryan, Siobhan
author_facet Ryan, Siobhan
author_sort Ryan, Siobhan
title An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds
title_short An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds
title_full An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds
title_fullStr An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds
title_full_unstemmed An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds
title_sort investigation into the biochemical effects of heavy metal exposure on seaweeds
publishDate 2010
url https://repository.wit.ie/1654/
https://repository.wit.ie/1654/1/Siobhan_Ryan_Thesis.pdf
genre Newfoundland
genre_facet Newfoundland
op_relation https://repository.wit.ie/1654/1/Siobhan_Ryan_Thesis.pdf
Ryan, Siobhan (2010) An Investigation into the Biochemical Effects of Heavy Metal Exposure on Seaweeds. PhD thesis, Waterford Institute of Technology.
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