Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast
Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting hap...
Published in: | Diseases of Aquatic Organisms |
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ftwilliammarycol:oai:scholarworks.wm.edu:vimsarticles-2584 2023-06-11T04:11:04+02:00 Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast Renault, R Stokes, NA Burreson, EM 2000-08-01T07:00:00Z application/pdf https://scholarworks.wm.edu/vimsarticles/1577 doi: 10.3354/dao042207 https://scholarworks.wm.edu/context/vimsarticles/article/2584/viewcontent/renault2000.pdf unknown W&M ScholarWorks https://scholarworks.wm.edu/vimsarticles/1577 doi: 10.3354/dao042207 https://scholarworks.wm.edu/context/vimsarticles/article/2584/viewcontent/renault2000.pdf VIMS Articles Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries text 2000 ftwilliammarycol https://doi.org/10.3354/dao042207 2023-05-04T17:45:09Z Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A series of primers was designed to amplify short regions of small subunit ribosomal DNA (SSU rDNA) from most haplosporidians. The primers encompass a highly variable region of the SSU rDNA and did not amplify oyster DNA. PCR amplification of the infected C, gigas genomic DNA with these primers yielded the expected-sized product from the primer pair targeting the shortest region (94 bp). This PCR product was sequenced and it was identical to the corresponding SSU rDNA region of H. nelsoni. Text Crassostrea gigas Pacific oyster W&M ScholarWorks Pacific Diseases of Aquatic Organisms 42 207 214 |
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Open Polar |
collection |
W&M ScholarWorks |
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ftwilliammarycol |
language |
unknown |
topic |
Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
spellingShingle |
Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries Renault, R Stokes, NA Burreson, EM Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast |
topic_facet |
Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
description |
Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A series of primers was designed to amplify short regions of small subunit ribosomal DNA (SSU rDNA) from most haplosporidians. The primers encompass a highly variable region of the SSU rDNA and did not amplify oyster DNA. PCR amplification of the infected C, gigas genomic DNA with these primers yielded the expected-sized product from the primer pair targeting the shortest region (94 bp). This PCR product was sequenced and it was identical to the corresponding SSU rDNA region of H. nelsoni. |
format |
Text |
author |
Renault, R Stokes, NA Burreson, EM |
author_facet |
Renault, R Stokes, NA Burreson, EM |
author_sort |
Renault, R |
title |
Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast |
title_short |
Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast |
title_full |
Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast |
title_fullStr |
Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast |
title_full_unstemmed |
Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast |
title_sort |
haplosporidiosis in the pacific oyster crassostrea gigas from the french atlantic coast |
publisher |
W&M ScholarWorks |
publishDate |
2000 |
url |
https://scholarworks.wm.edu/vimsarticles/1577 https://scholarworks.wm.edu/context/vimsarticles/article/2584/viewcontent/renault2000.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
VIMS Articles |
op_relation |
https://scholarworks.wm.edu/vimsarticles/1577 doi: 10.3354/dao042207 https://scholarworks.wm.edu/context/vimsarticles/article/2584/viewcontent/renault2000.pdf |
op_doi |
https://doi.org/10.3354/dao042207 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
42 |
container_start_page |
207 |
op_container_end_page |
214 |
_version_ |
1768385920580452352 |