Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland
Parasitic dinoflagellates in the genus Hematodinium infect a number of decapod crustaceans in waters off the UK, including the Norway lobster Nephrops norvegicus and the edible crab Cancerpagurus. This study investigated sequence variability in the first internal transcribed spacer (ITS1) region of...
Published in: | Diseases of Aquatic Organisms |
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ftwilliammarycol:oai:scholarworks.wm.edu:vimsarticles-2015 2023-06-11T04:10:57+02:00 Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland Small, Hamish J Shields, Jeffrey D. Moss, JA Reece, Kimberly S 2007-01-01T08:00:00Z application/pdf https://scholarworks.wm.edu/vimsarticles/1015 doi: 10.3354/dao075251 https://scholarworks.wm.edu/context/vimsarticles/article/2015/viewcontent/d075p251.pdf unknown W&M ScholarWorks https://scholarworks.wm.edu/vimsarticles/1015 doi: 10.3354/dao075251 https://scholarworks.wm.edu/context/vimsarticles/article/2015/viewcontent/d075p251.pdf VIMS Articles Lobster Nephrops-Norvegicus Bitter Crab Disease 5.8S Ribosomal-Rna Perkinsus-Marinus Parasitic Dinoflagellate West-Coast Dinophyceae Dna Variability Prevalence Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries text 2007 ftwilliammarycol https://doi.org/10.3354/dao075251 2023-05-04T17:43:38Z Parasitic dinoflagellates in the genus Hematodinium infect a number of decapod crustaceans in waters off the UK, including the Norway lobster Nephrops norvegicus and the edible crab Cancerpagurus. This study investigated sequence variability in the first internal transcribed spacer (ITS1) region of the ribosomal RNA complex of Hematodinium spp. infecting N. norvegicus, C. pagurus, and Pagurus bernhardus from 4 locations in the UK and from the Hematodinium sp. infecting Chionoecetes opilio from the province of Newfoundland and Labrador, Canada. Phylogenetic analysis of the Hema todinium ITS 1 sequences from N. norvegicus, C. pagurus, P. bernhardus and C. opilio suggest that these crustaceans are infected with the same species of Hematodinium. Length variability of the ITS1 region was observed (324 to 345 bp) and attributed to 4 variable microsatellite regions (CATG)(n), (GCC)(n)TCCGC(TG)(n), (TA)(n), and (GAA)(n)(GGA)(n) within the sequenced ITS1 fragment. The observed variation may be due to co-infection of the host crustacean with several different strains of Hematodinium or differences among copies of ITS1 region within the genome of a single parasite cell. The Hematodinium ITS1 sequence from N. norvegicus, C. pagurus, P. bernhardus and C. opilio isolates was sufficiently conserved in primer binding regions targeted by previous molecular diagnostic assays; therefore, we suggest that this assay could be used to screen for Hematodinium infections in these crustacean hosts. Text Chionoecetes opilio Newfoundland W&M ScholarWorks Newfoundland Canada Norway Diseases of Aquatic Organisms 75 251 258 |
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Open Polar |
collection |
W&M ScholarWorks |
op_collection_id |
ftwilliammarycol |
language |
unknown |
topic |
Lobster Nephrops-Norvegicus Bitter Crab Disease 5.8S Ribosomal-Rna Perkinsus-Marinus Parasitic Dinoflagellate West-Coast Dinophyceae Dna Variability Prevalence Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
spellingShingle |
Lobster Nephrops-Norvegicus Bitter Crab Disease 5.8S Ribosomal-Rna Perkinsus-Marinus Parasitic Dinoflagellate West-Coast Dinophyceae Dna Variability Prevalence Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries Small, Hamish J Shields, Jeffrey D. Moss, JA Reece, Kimberly S Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland |
topic_facet |
Lobster Nephrops-Norvegicus Bitter Crab Disease 5.8S Ribosomal-Rna Perkinsus-Marinus Parasitic Dinoflagellate West-Coast Dinophyceae Dna Variability Prevalence Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
description |
Parasitic dinoflagellates in the genus Hematodinium infect a number of decapod crustaceans in waters off the UK, including the Norway lobster Nephrops norvegicus and the edible crab Cancerpagurus. This study investigated sequence variability in the first internal transcribed spacer (ITS1) region of the ribosomal RNA complex of Hematodinium spp. infecting N. norvegicus, C. pagurus, and Pagurus bernhardus from 4 locations in the UK and from the Hematodinium sp. infecting Chionoecetes opilio from the province of Newfoundland and Labrador, Canada. Phylogenetic analysis of the Hema todinium ITS 1 sequences from N. norvegicus, C. pagurus, P. bernhardus and C. opilio suggest that these crustaceans are infected with the same species of Hematodinium. Length variability of the ITS1 region was observed (324 to 345 bp) and attributed to 4 variable microsatellite regions (CATG)(n), (GCC)(n)TCCGC(TG)(n), (TA)(n), and (GAA)(n)(GGA)(n) within the sequenced ITS1 fragment. The observed variation may be due to co-infection of the host crustacean with several different strains of Hematodinium or differences among copies of ITS1 region within the genome of a single parasite cell. The Hematodinium ITS1 sequence from N. norvegicus, C. pagurus, P. bernhardus and C. opilio isolates was sufficiently conserved in primer binding regions targeted by previous molecular diagnostic assays; therefore, we suggest that this assay could be used to screen for Hematodinium infections in these crustacean hosts. |
format |
Text |
author |
Small, Hamish J Shields, Jeffrey D. Moss, JA Reece, Kimberly S |
author_facet |
Small, Hamish J Shields, Jeffrey D. Moss, JA Reece, Kimberly S |
author_sort |
Small, Hamish J |
title |
Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland |
title_short |
Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland |
title_full |
Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland |
title_fullStr |
Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland |
title_full_unstemmed |
Conservation in the first internal transcribed spacer region (ITS1) in Hematodinium species infecting crustacean hosts found in the UK and Newfoundland |
title_sort |
conservation in the first internal transcribed spacer region (its1) in hematodinium species infecting crustacean hosts found in the uk and newfoundland |
publisher |
W&M ScholarWorks |
publishDate |
2007 |
url |
https://scholarworks.wm.edu/vimsarticles/1015 https://scholarworks.wm.edu/context/vimsarticles/article/2015/viewcontent/d075p251.pdf |
geographic |
Newfoundland Canada Norway |
geographic_facet |
Newfoundland Canada Norway |
genre |
Chionoecetes opilio Newfoundland |
genre_facet |
Chionoecetes opilio Newfoundland |
op_source |
VIMS Articles |
op_relation |
https://scholarworks.wm.edu/vimsarticles/1015 doi: 10.3354/dao075251 https://scholarworks.wm.edu/context/vimsarticles/article/2015/viewcontent/d075p251.pdf |
op_doi |
https://doi.org/10.3354/dao075251 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
75 |
container_start_page |
251 |
op_container_end_page |
258 |
_version_ |
1768385721244057600 |