Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus
To investigate whether sublethal heat shock protects Perkinsus marinus (Dermo)infected oysters Crassostrea virginica from lethal heat stress, and the effects of P. marinus infection on sublethal heat shock response, oysters were first experimentally challenged with P. marinus. Then, when infections...
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ftwilliammarycol:oai:scholarworks.wm.edu:vimsarticles-2007 2023-06-11T04:15:49+02:00 Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus Encomio, VG Chu, FLE 2007-01-01T08:00:00Z application/pdf https://scholarworks.wm.edu/vimsarticles/1007 doi: 10.3354/dao076251 https://scholarworks.wm.edu/context/vimsarticles/article/2007/viewcontent/d076p251.pdf unknown W&M ScholarWorks https://scholarworks.wm.edu/vimsarticles/1007 doi: 10.3354/dao076251 https://scholarworks.wm.edu/context/vimsarticles/article/2007/viewcontent/d076p251.pdf VIMS Articles Pacific Oyster Induced Thermotolerance Mytilus-Edulis In-Vitro Gigas Induction Disease Heat-Shock-Protein-70 Temperature Apicomplexa Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries text 2007 ftwilliammarycol https://doi.org/10.3354/dao076251 2023-05-04T17:43:38Z To investigate whether sublethal heat shock protects Perkinsus marinus (Dermo)infected oysters Crassostrea virginica from lethal heat stress, and the effects of P. marinus infection on sublethal heat shock response, oysters were first experimentally challenged with P. marinus. Then, when infections in oysters progressed to moderate levels (parasite burden = 10(4) to 10(5) cells g(-1) wet tissue weight), oysters were treated with a sublethal heat shock at 40 C for 1 h (heat shock + Dermo challenge). Other treatment groups included heat-shocked, unchallenged (non-P. marinus challenged) oysters and non-heat-shocked, P. marinus-challenged and -unchallenged oysters. Thermal tolerance was compared among these treatments by administering a lethal heat treatment at 44 C for 1 h, 7 d after sublethal heat shock. Sublethal heat shock enhanced survival to lethal heat treatment in both P. marinus-challenged and -unchallenged oysters. Although levels of hsp70 isoforms (hsp69 and hsp72) did not vary significantly by heat shock or infection with P. marinus, responses due to these treatments were apparent when comparing hsp70 levels within infected and uninfected oysters. Infection enhanced expression of hsp69, regardless of whether oysters were heat shocked or not. In uninfected oysters, hsp72 increased due to heat shock 2 and 7 d post heat shock. Overall, this study demonstrates that heat shock can improve survival in oysters, even in oysters infected with P, marinus. Expression of hsp70 varied among isoforms after sublethal and lethal heat shocks and in infected and uninfected oysters. The heat shock response was not negatively affected by P. marinus infection. Text Pacific oyster W&M ScholarWorks Pacific Diseases of Aquatic Organisms 76 251 260 |
institution |
Open Polar |
collection |
W&M ScholarWorks |
op_collection_id |
ftwilliammarycol |
language |
unknown |
topic |
Pacific Oyster Induced Thermotolerance Mytilus-Edulis In-Vitro Gigas Induction Disease Heat-Shock-Protein-70 Temperature Apicomplexa Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
spellingShingle |
Pacific Oyster Induced Thermotolerance Mytilus-Edulis In-Vitro Gigas Induction Disease Heat-Shock-Protein-70 Temperature Apicomplexa Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries Encomio, VG Chu, FLE Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus |
topic_facet |
Pacific Oyster Induced Thermotolerance Mytilus-Edulis In-Vitro Gigas Induction Disease Heat-Shock-Protein-70 Temperature Apicomplexa Aquatic Health Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
description |
To investigate whether sublethal heat shock protects Perkinsus marinus (Dermo)infected oysters Crassostrea virginica from lethal heat stress, and the effects of P. marinus infection on sublethal heat shock response, oysters were first experimentally challenged with P. marinus. Then, when infections in oysters progressed to moderate levels (parasite burden = 10(4) to 10(5) cells g(-1) wet tissue weight), oysters were treated with a sublethal heat shock at 40 C for 1 h (heat shock + Dermo challenge). Other treatment groups included heat-shocked, unchallenged (non-P. marinus challenged) oysters and non-heat-shocked, P. marinus-challenged and -unchallenged oysters. Thermal tolerance was compared among these treatments by administering a lethal heat treatment at 44 C for 1 h, 7 d after sublethal heat shock. Sublethal heat shock enhanced survival to lethal heat treatment in both P. marinus-challenged and -unchallenged oysters. Although levels of hsp70 isoforms (hsp69 and hsp72) did not vary significantly by heat shock or infection with P. marinus, responses due to these treatments were apparent when comparing hsp70 levels within infected and uninfected oysters. Infection enhanced expression of hsp69, regardless of whether oysters were heat shocked or not. In uninfected oysters, hsp72 increased due to heat shock 2 and 7 d post heat shock. Overall, this study demonstrates that heat shock can improve survival in oysters, even in oysters infected with P, marinus. Expression of hsp70 varied among isoforms after sublethal and lethal heat shocks and in infected and uninfected oysters. The heat shock response was not negatively affected by P. marinus infection. |
format |
Text |
author |
Encomio, VG Chu, FLE |
author_facet |
Encomio, VG Chu, FLE |
author_sort |
Encomio, VG |
title |
Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus |
title_short |
Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus |
title_full |
Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus |
title_fullStr |
Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus |
title_full_unstemmed |
Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus |
title_sort |
heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters crassostrea virginica infected with the parasite perkinsus marinus |
publisher |
W&M ScholarWorks |
publishDate |
2007 |
url |
https://scholarworks.wm.edu/vimsarticles/1007 https://scholarworks.wm.edu/context/vimsarticles/article/2007/viewcontent/d076p251.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
VIMS Articles |
op_relation |
https://scholarworks.wm.edu/vimsarticles/1007 doi: 10.3354/dao076251 https://scholarworks.wm.edu/context/vimsarticles/article/2007/viewcontent/d076p251.pdf |
op_doi |
https://doi.org/10.3354/dao076251 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
76 |
container_start_page |
251 |
op_container_end_page |
260 |
_version_ |
1768372945873272832 |