Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA)
Monoclonal antibodies specific to a heat shock protein 70-like protein expressed in the eggs of Macoma balthica (mb-HSP70) were employed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify fecundity in females. The assay was specific to egg tissue, with no demonstrable reactivity wit...
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Online Access: | https://scholarworks.wm.edu/vimsarticles/994 https://scholarworks.wm.edu/context/vimsarticles/article/1994/viewcontent/Long_2008_Quantifying_fecundity_in_macoma_bal.pdf |
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ftwilliammarycol:oai:scholarworks.wm.edu:vimsarticles-1994 2023-06-11T04:14:44+02:00 Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) Long, WC Bromage, E Seitz, RD Kaattari, S 2008-01-01T08:00:00Z application/pdf https://scholarworks.wm.edu/vimsarticles/994 doi: 10.3354/ab00075 https://scholarworks.wm.edu/context/vimsarticles/article/1994/viewcontent/Long_2008_Quantifying_fecundity_in_macoma_bal.pdf unknown W&M ScholarWorks https://scholarworks.wm.edu/vimsarticles/994 doi: 10.3354/ab00075 https://scholarworks.wm.edu/context/vimsarticles/article/1994/viewcontent/Long_2008_Quantifying_fecundity_in_macoma_bal.pdf VIMS Articles Reproductive Output Water Temperature Immersion Time Chesapeake Bay Wadden Sea Bivalvia Hsp70 Clam Communities Oogenesis Aquatic Health Sciences Peer-Reviewed Articles Biological Sciences Peer-Reviewed Articles Aquaculture and Fisheries text 2008 ftwilliammarycol https://doi.org/10.3354/ab00075 2023-05-04T17:43:38Z Monoclonal antibodies specific to a heat shock protein 70-like protein expressed in the eggs of Macoma balthica (mb-HSP70) were employed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify fecundity in females. The assay was specific to egg tissue, with no demonstrable reactivity with juvenile or male tissues. The concentration of mb-HSP70 increased as the female gonads matured, necessitating the determination of a calibration curve for future experiments. The number of eggs in females was positively correlated with body mass index (BMI), and clams with a BMI < 1.4 did not produce eggs. The estimated number of eggs per clam during the fall spawning period was similar to that observed in eastern North Atlantic populations in clams of similar size; clams with shell lengths from 16 to 18 mm had from 7000 to 60 000 eggs apiece. Larger clams had up to 450 000 eggs. This assay effectively quantifies eggs at any stage of gonadal development in which eggs can be distinguished microscopically and is easier and cheaper to perform than other techniques of comparable precision. Text North Atlantic W&M ScholarWorks Aquatic Biology 3 2 187 193 |
institution |
Open Polar |
collection |
W&M ScholarWorks |
op_collection_id |
ftwilliammarycol |
language |
unknown |
topic |
Reproductive Output Water Temperature Immersion Time Chesapeake Bay Wadden Sea Bivalvia Hsp70 Clam Communities Oogenesis Aquatic Health Sciences Peer-Reviewed Articles Biological Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
spellingShingle |
Reproductive Output Water Temperature Immersion Time Chesapeake Bay Wadden Sea Bivalvia Hsp70 Clam Communities Oogenesis Aquatic Health Sciences Peer-Reviewed Articles Biological Sciences Peer-Reviewed Articles Aquaculture and Fisheries Long, WC Bromage, E Seitz, RD Kaattari, S Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) |
topic_facet |
Reproductive Output Water Temperature Immersion Time Chesapeake Bay Wadden Sea Bivalvia Hsp70 Clam Communities Oogenesis Aquatic Health Sciences Peer-Reviewed Articles Biological Sciences Peer-Reviewed Articles Aquaculture and Fisheries |
description |
Monoclonal antibodies specific to a heat shock protein 70-like protein expressed in the eggs of Macoma balthica (mb-HSP70) were employed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify fecundity in females. The assay was specific to egg tissue, with no demonstrable reactivity with juvenile or male tissues. The concentration of mb-HSP70 increased as the female gonads matured, necessitating the determination of a calibration curve for future experiments. The number of eggs in females was positively correlated with body mass index (BMI), and clams with a BMI < 1.4 did not produce eggs. The estimated number of eggs per clam during the fall spawning period was similar to that observed in eastern North Atlantic populations in clams of similar size; clams with shell lengths from 16 to 18 mm had from 7000 to 60 000 eggs apiece. Larger clams had up to 450 000 eggs. This assay effectively quantifies eggs at any stage of gonadal development in which eggs can be distinguished microscopically and is easier and cheaper to perform than other techniques of comparable precision. |
format |
Text |
author |
Long, WC Bromage, E Seitz, RD Kaattari, S |
author_facet |
Long, WC Bromage, E Seitz, RD Kaattari, S |
author_sort |
Long, WC |
title |
Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) |
title_short |
Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) |
title_full |
Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) |
title_fullStr |
Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) |
title_full_unstemmed |
Quantifying fecundity in Macoma balthica using an enzyme-linked immunosorbent assay (ELISA) |
title_sort |
quantifying fecundity in macoma balthica using an enzyme-linked immunosorbent assay (elisa) |
publisher |
W&M ScholarWorks |
publishDate |
2008 |
url |
https://scholarworks.wm.edu/vimsarticles/994 https://scholarworks.wm.edu/context/vimsarticles/article/1994/viewcontent/Long_2008_Quantifying_fecundity_in_macoma_bal.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
VIMS Articles |
op_relation |
https://scholarworks.wm.edu/vimsarticles/994 doi: 10.3354/ab00075 https://scholarworks.wm.edu/context/vimsarticles/article/1994/viewcontent/Long_2008_Quantifying_fecundity_in_macoma_bal.pdf |
op_doi |
https://doi.org/10.3354/ab00075 |
container_title |
Aquatic Biology |
container_volume |
3 |
container_issue |
2 |
container_start_page |
187 |
op_container_end_page |
193 |
_version_ |
1768370984720531456 |